Decoding social behaviors in a glycerol dependent bacterial consortium during Reactive Blue 28 degradation

Nanjani, Sandhya; Rawal, Khushboo; Keharia, Haresh

doi:10.1007/s42770-020-00303-3

Cited by 5 publications

(9 citation statements)

References 52 publications

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“…APG4 contained the highest number of ORFs for NADH: DCIP oxidoreductase (i.e., 3), thus indicating its greater contribution in the degradation of dye under the reductive condition as compared to the others. These results corroborated with previously reported NADH-DCIP reductase activities of APG isolates [ 24 ]. Amongst all isolates, APG4 possessed more than 18-fold higher reductase activity in comparison to APG1 and APG2.…”

Section: Resultssupporting

confidence: 93%

“…Laccases are copper-containing oxidases catalyzing the oxidation of aromatics coupled to the synthesis of water as a by-product [ 51 ]. Notably, none of the genomes possessed genes encoding this enzyme which is in agreement with our experimental results [ 24 ]. However, Cytochrome P450 (CYP450) has been reported to metabolize a range of xenobiotic compounds, including dye, by hydroxylation reactions [ 52 ].…”

Section: Resultssupporting

confidence: 91%

“…6 ). Previously, it has been reported that APG4 possessed the NADH-dependent MR reductase activity [ 24 ]. Two types of NADH dehydrogenases involved in the dye-reduction were detected in the members of the consortium.…”

Section: Resultsmentioning

confidence: 99%

“…The RB28 degradation efficiency (i.e., in terms of percent degradation and rate) was found to be maximum when all three strains were present in the system. Interestingly, the same isolates could not degrade the dye individually, indicating the prevalence of strong cooperation within the members during the process [ 24 ]. The comparison of subsystems obtained from RAST revealed that APG4 might be central in dye reduction as it had maximum features for “electron-donating reactions”.…”

Section: Discussionmentioning

confidence: 99%

“…APG4) was developed from a mixed-culture enriched on medium containing 100 mg Reactive Blue 28/L and 0.1% (v/v) glycerol as a co-substrate under the anoxic condition with repeated transfers for several months. The three isolates from the mixed culture were selected based on the functional and social-behavioral traits [ 24 ]. In order to gain a deeper understanding about the role of each member of the consortium in dye degradation, the genomes of all the three members of consortium SCP were sequenced and analyzed.…”

Section: Introductionmentioning

confidence: 99%

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Genome analysis to decipher syntrophy in the bacterial consortium ‘SCP’ for azo dye degradation

2021

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Background A bacterial consortium SCP comprising three bacterial members, viz. Stenotrophomonas acidaminiphila APG1, Pseudomonas stutzeri APG2 and Cellulomonas sp. APG4 was developed for degradation of the mono-azo dye, Reactive Blue 28. The genomic analysis of each member of the SCP consortium was done to elucidate the catabolic potential and role of the individual organism in dye degradation. Results The genes for glycerol utilization were detected in the genomes of APG2 and APG4, which corroborated with their ability to grow on a minimal medium containing glycerol as the sole co-substrate. The genes for azoreductase were identified in the genomes of APG2 and APG4, while no such trait could be determined in APG1. In addition to co-substrate oxidation and dye reduction, several other cellular functions like chemotaxis, signal transduction, stress-tolerance, repair mechanisms, aromatic degradation, and copper tolerance associated with dye degradation were also annotated. A model for azo dye degradation is postulated, representing the predominant role of APG4 and APG2 in dye metabolism while suggesting an accessory role of APG1. Conclusions This exploratory study is the first-ever attempt to divulge the genetic basis of azo-dye co-metabolism by cross-genome comparisons and can be harnessed as an example for demonstrating microbial syntrophy.

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Section: Resultssupporting

confidence: 93%