Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato

Nahirñak, Vanesa; Rivarola, Máximo Lisandro; Almasia, Natalia Inés; Barón, María Pilar Barrios; Hopp, H. Esteban; Vile, Denis; Paniego, Norma; Rovere, Cecilia Vázquez

doi:10.1371/journal.pone.0214165

Cited by 32 publications

(30 citation statements)

References 64 publications

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“…The Snakin/GASA family members show divergent expression patterns in regard to spatial and temporal regulation. In potato, the transcripts of StSN1 are found to be particularly abundant in axillary, stem, floral buds, and in fully developed petals, nevertheless no expression has been detected in roots, stolons or leaves [ 40 , 65 ]. Moreover, the StSN1 promoter expressed mainly in young tissues and zones (e.g., shoot apex, apical bud, vascular stem and root tissues, etc.)…”

Section: Spatiotemporal Expression Of the Snakin/gasamentioning

confidence: 99%

“…associated with vigorous growth and cell division, and it is active in young stages gradually decreasing as the plant ages. Accordingly, at the protein level StSN1 is present mainly in young tissues associated with active growth and cell division zones [ 65 ]. The steady-state mRNA levels of StSN2 are high in tubers, flowers, roots and leaves [ 35 , 55 ].…”

Section: Spatiotemporal Expression Of the Snakin/gasamentioning

confidence: 99%

“…Aside from responsiveness to GA and ABA, it has been found that OsGSR1 directly regulates the brassinosteroid (BR) biosynthesis and signaling pathway [ 59 , 73 ]. The Snakin-1 silenced transgenic potato plants causes accumulation of reactive oxygen species (ROS), significant reduction of ABA, increase of salicylic acid (SA) and GA and downregulation of sterol biosynthesis [ 65 ](. The transcript of HbGASA7-1 , 14 and 16 is significantly upregulated after the treatment with ethylene (ETH), SA, or jasmonic acid (JA) [ 56 ].…”

Section: Hormonal Regulation Of the Snakin/gasa Genesmentioning

confidence: 99%

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Molecular and Biological Properties of Snakins: The Foremost Cysteine-Rich Plant Host Defense Peptides

Han

Cao

et al. 2020

JoF

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Plant host defense peptides (HDPs), also known as antimicrobial peptides (AMPs), are regarded as one of the most prevalent barriers elaborated by plants to combat various infective agents. Among the multiple classes of HDPs, the Snakin class attracts special concern, as they carry 12 cysteine residues, being the foremost cysteine-rich peptides of the plant HDPs. Also, their cysteines are present at very highly conserved positions and arranged in an extremely similar way among different members. Like other plant HDPs, Snakins have been shown to exhibit strong antifungal and antibacterial activity against a wide range of plant pathogens. Moreover, they display diversified biological activities in many aspects of plant growth and the development process. This review is devoted to present the general characters of the Snakin class of plant HDPs, as well as the individual features of different Snakin family members. Specifically, the sequence properties, spatial structures, distributions, expression patterns and biological activities of Snakins are described. In addition, further detailed classification of the Snakin family members, along with their possible mode of action and potential applications in the field of agronomy and pathology are discussed.

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Section: Spatiotemporal Expression Of the Snakin/gasamentioning

confidence: 99%

Section: Spatiotemporal Expression Of the Snakin/gasamentioning

confidence: 99%

Section: Hormonal Regulation Of the Snakin/gasa Genesmentioning

confidence: 99%

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Molecular and Biological Properties of Snakins: The Foremost Cysteine-Rich Plant Host Defense Peptides

Han

Cao

et al. 2020

JoF

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“…As a result of the signal transduction, a number of transcription factors are activated (Asai et al, 2002;Molina et al, 2008). Some of them are specific to a certain stress factor -for example, the transcription factors DREB2A and DREB2B are induced during drought and salinity, and OsCDPK13 is only activated by cold (Abbasi et al, 2004;Nakashima et al, 2000) -while others are nonspecifically activated in response to various abiotic factors. The activated TFs then start/suppress transcription of genes whose products regulate the stress response, thus repairing the damage caused by stress and increasing further resistance to a particular stress factor.…”

Section: Lipid Transfer Proteinsmentioning

confidence: 99%

The role of plant antimicrobial peptides (AMPs) in response to biotic and abiotic environmental factors

Kulaeva

Kliukova

Afonin

et al. 2020

BioComm

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Plants are continuously exposed to various biotic and abiotic factors that may trigger cascade reactions aimed at maintaining homeostasis. One of the most important components of plant protection from biotic factors is the synthesis of antimicrobial peptides (AMPs). AMPs are a large group of peptides present in insects, animals and plants. Plant innate immunity is provided by AMPs from different families that are categorized according to sequence similarity, the number and order of amino acid residues, and the tertiary structure of the mature peptide. AMPs may also participate in plant response to abiotic stresses such as high salinity, drought, high or low temperature, and heavy metals. In nitrogen-fixing nodules of some members of the Fabaceae family, AMP-like molecules named NCR peptides promote the differentiation of the symbiotic bacteria into bacteroids. Thus, AMPs are used by plants for fine tuning their responses to biotic and abiotic factors alike.

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“…Young leaves of 3-4 week-old N. benthamiana plants were coinfiltrated with an equal mix of both cultures. For this assay, as previously described by Nahirñak et al (2019), StDIM/DWF1 fused at its carboxy-terminus to the two fragments (N-or C-terminal) of the Venus protein in combination with Snakin-1 fused to the C-terminal or the N-terminus fragment of Venus as C-terminal protein fusion were used as positive controls.…”

Section: Yeast Two-hybrid Assays and Bimolecular Fluorescence Complmentioning

confidence: 99%

Argentinian potato leafroll virus P0 protein: Novel activities for a previously known suppressor

et al. 2020

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The potato leafroll virus (PLRV) P0 protein (P0PL) is a suppressor of RNA silencing. In this study, we showed that P0 protein from an Argentinian isolate of PLRV (P0PL‐Ar) has an additional activity not described for other PLRV or P0 proteins from poleroviruses. Besides reporting that P0PL‐Ar displays both local and systemic silencing suppressor activity, we demonstrated, for the first time, that P0PL‐Ar impedes accumulation of dsRNA‐derived siRNAs. We also showed that P0PL‐Ar interacts with Solanum tuberosum SKP1 orthologue (StSKP1) and triggers destabilization of ARGONAUTE 1 (AGO1) and that these actions are mediated by the F‐box‐like domain. A mutant in the GW/WG motif within the P0PL‐Ar F‐box‐like motif lost the suppression activity, the interaction with StSKP1 and abolished AGO1 decay. Interestingly, a mutant in the L76/P77 residues within the P0PL‐Ar F‐box‐like motif, which lost the suppression activity and the interaction with StSKP1, retained the capacity to enable AGO1 decay. Thus, unlike other P0 proteins of previously characterized poleroviruses, P0PL‐Ar seems to have a dual activity, according to the findings of this study. This protein would act at both an upstream and a downstream step of the RNA silencing pathway: upstream of Dicer‐like enzyme (DCL)‐mediated primary siRNA production and downstream at the RNA‐induced silencing complex (RISC) complex level. Our results contribute to the understanding of the different ways PLRV P0 proteins function as silencing suppressors.

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Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato

Cited by 32 publications

References 64 publications

Molecular and Biological Properties of Snakins: The Foremost Cysteine-Rich Plant Host Defense Peptides

Molecular and Biological Properties of Snakins: The Foremost Cysteine-Rich Plant Host Defense Peptides

The role of plant antimicrobial peptides (AMPs) in response to biotic and abiotic environmental factors

Argentinian potato leafroll virus P0 protein: Novel activities for a previously known suppressor

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