Snake venomics and antivenomics of Bothrops colombiensis, a medically important pitviper of the Bothrops atrox-asper complex endemic to Venezuela: Contributing to its taxonomy and snakebite management

Calvete, Juan J.; Borges, Arnaldo Chaer; Segura, Álvaro; Flores-Dı́az, Marietta; Alape-Girón, Alberto; Gutiérrez, José Marı́a; Dı́ez, Nardy; Sousa, Leonardo De; Kiriakos, Demetrio; Sanchez, Eládio Flores; Faks, J.; Escolano, José; Sanz, Libia

doi:10.1016/j.jprot.2009.01.005

Cited by 75 publications

(70 citation statements)

References 43 publications

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“…21 , 31, 43 According to their antivenomic immunoreactivity profile, venom proteins were classified as C-toxins ( c ompletely immunodepleted toxins), P-toxins ( p artly immunodepleted toxins), and N-toxins ( n on-immunodepleted toxins). 39 Our results show that the vast majority of venom components, in all venoms tested, are C-toxins ( Figures 1 and 2 ). Based on data accrued from previous proteomic analyses of these venoms, these C-toxins comprise mainly P-I and P-III snake venom metalloproteinases (SVMPs), serine Figure 1.…”

Section: Resultsmentioning

confidence: 61%

“…com ) against a private database containing 1,083 viperid protein sequences deposited in the SwissProt/TrEMBL database (UniProtKB/Swiss-Prot Release 56.7 of 20-Jan-2009; http:// us.expasy.org/sprot/ ) plus the previously assigned peptide ion sequences from snake venomics projects carried out in our laboratory. 21,[28][29][30]31,[36][37][38][39][40][41][42][43] MS/MS mass tolerance was set to ±0.6 Da. Carbamidomethyl cysteine and oxidation of methionine were fixed and variable modifications, respectively.…”

Section: Venomsmentioning

confidence: 99%

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Antivenomic Assessment of the Immunological Reactivity of EchiTAb-Plus-ICP, an Antivenom for the Treatment of Snakebite Envenoming in Sub-Saharan Africa

Calvete¹,

Cid²,

Sanz³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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The immunoreactivity of EchiTAb-Plus-ICP, an antivenom developed for the treatment of snakebite envenoming in sub-Saharan Africa, to venoms of seven Echis and Bitis species, was assessed by “antivenomics.” This proteomic approach is based on the ability of an antivenom to immunodeplete homologous or heterologous venom proteins. Our results show an extensive cross-reactivity of this antivenom against all Echis and Bitis venoms studied, as revealed by the complete immunodepletion of the majority of venom components, including metalloproteinases, serine proteinases, C-type lectin-like proteins, some phospholipases A2 and L-amino acid oxidase. However, some phospholipases A2, disintegrins and proteinase inhibitors were immunodepleted to only a partial extent. These results support the hypothesis that immunizing horses with a mixture of the venoms of Echis ocellatus, Bitis arietans, and Naja nigricollis generates antibodies capable of recognizing the majority of components of medically-relevant homologous and heterologous viperid venoms of the genera Bitis and Echis from sub-Saharan Africa.

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Section: Resultsmentioning

confidence: 61%

Section: Venomsmentioning

confidence: 99%

Antivenomic Assessment of the Immunological Reactivity of EchiTAb-Plus-ICP, an Antivenom for the Treatment of Snakebite Envenoming in Sub-Saharan Africa

Calvete¹,

Cid²,

Sanz³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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“…Calvete and co-workers from Venezuela and Costa Rica have compared the venom proteomes of B. colombiensis, B. asper, and B. atrox using venomics and antivenomics approaches [61]. This study did not support the suggested synonymy between B. colombiensis and B. atrox.…”

Section: Antivenomics: Proteomic Tools For Studying the Immunologicalmentioning

confidence: 99%

Venoms, venomics, antivenomics

et al. 2009

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a b s t r a c tVenoms comprise mixtures of peptides and proteins tailored by Natural Selection to act on vital systems of the prey or victim. Here we review our proteomic protocols for uncoiling the composition, immunological profile, and evolution of snake venoms. Our long-term goal is to gain a deep insight of all viperid venom proteomes. Knowledge of the inter-and intraspecies ontogenetic, individual, and geographic venom variability has applied importance for the design of immunization protocols aimed at producing more effective polyspecific antivenoms. A practical consequence of assessing the cross-reactivity of heterologous antivenoms is the possibility of circumventing the restricted availability of species-specific antivenoms in some regions. Further, the high degree of target specificity makes toxins valuable scaffolds for drug development.

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“…A robust knowledge of venom composition may aid in establishing ecological and taxonomic correlates [31][32][33][34][35][36][37][38][39] useful for rationalizing the pathological effects of envenomation [40]. Research on venoms has been continuously improved by advances in technology.…”

Section: Introductionmentioning

confidence: 99%

Snake venomics of two poorly known Hydrophiinae: Comparative proteomics of the venoms of terrestrial Toxicocalamus longissimus and marine Hydrophis cyanocinctus

Calvete

Ghezellou

Paiva

et al. 2012

Journal of Proteomics

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Snake venomics and antivenomics of Bothrops colombiensis, a medically important pitviper of the Bothrops atrox-asper complex endemic to Venezuela: Contributing to its taxonomy and snakebite management

Cited by 75 publications

References 43 publications

Antivenomic Assessment of the Immunological Reactivity of EchiTAb-Plus-ICP, an Antivenom for the Treatment of Snakebite Envenoming in Sub-Saharan Africa

Antivenomic Assessment of the Immunological Reactivity of EchiTAb-Plus-ICP, an Antivenom for the Treatment of Snakebite Envenoming in Sub-Saharan Africa

Venoms, venomics, antivenomics

Snake venomics of two poorly known Hydrophiinae: Comparative proteomics of the venoms of terrestrial Toxicocalamus longissimus and marine Hydrophis cyanocinctus

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