Snake venom metalloproteinases:Their role in the pathogenesis of local tissue damage

Gutiérrez, José Marı́a; Rucavado, Alexandra

doi:10.1016/s0300-9084(00)01163-9

Cited by 513 publications

(348 citation statements)

References 84 publications

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“…The pathogenesis of local and systemic effects induced by B. asper venom has been partially investigated. Various locallyacting toxins, mostly metalloproteinases and phospholipases A 2 , are responsible for the acute local pathological effects [5][6][7]. In addition, a variety of venom components, such as metalloproteinases, serine proteinases and proteins of the Ctype lectin family, induce defi brination, systemic bleeding and thrombocytopenia, therefore promoting profuse bleeding and coagulopathy [8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Role of nitric oxide in the local and systemic pathophysiological effects induced by Bothrops asper snake venom in mice

2006

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Abstract.Objective: To assess the role of nitric oxide in the most relevant local and systemic manifestations in mice injected with the venom of the snake Bothrops asper. Mice were pretreated with nitric oxide synthase inhibitors, and the modifi cations of the pathological effects induced by the venom were tested. Results: Inhibition of NO synthesis did not affect acute local myonecrosis and hemorrhage in muscle tissue upon intramuscular injection of venom. Local footpad edema was reduced in mice pretreated with the NO synthase inhibitor L-NAME, and a reduction in the extent of infl ammatory infi ltrate in muscle tissue was observed after envenomation in mice pretreated with L-NAME and aminoguanidine. The most pronounced effect of NOS inhibition by L-NAME was an increment in the lethal activity of the venom, when injected by the intraperitoneal route. Conclusion: Nitric oxide does not seem to play a signifi cant role in the local acute pathological alterations (hemorrhage and myonecrosis) induced by B. asper venom in mice, although it contributes to edema and infl ammatory infi ltrate. Nitric oxide exerts a protective role in the systemic pathophysiological manifestations leading to lethality.

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Section: Introductionmentioning

confidence: 99%

Role of nitric oxide in the local and systemic pathophysiological effects induced by Bothrops asper snake venom in mice

2006

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“…The myonecrosis by direct action is caused by myotoxic phopholipases A 2 , which affect the integrity of skeletal muscle cell plasma membranes, causing rapid hypercontraction and clumping of myofilaments (16). Indirect myonecrosis is generally associated with hemorrhagic SVMPs, inducing muscle damage via ischemia that develops secondary to their disruptive action on the vasculature (17).…”

Section: Discussionmentioning

confidence: 99%

Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study

Baldo

Ferreira²,

Lopes

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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Abstract:The damaging effects of neuwiedase, a non-hemorrhagic snake venom metalloproteinase from P-I class, on gastrocnemius muscle are studied herein. Following neuwiedase injection, ultrastructural alterations were detected early showing disarrangement of skeletal muscle fibers (characterized by discontinuity of Z lines), mitochondrial swelling, and disruption of plasma membrane and basal lamina. Degradation of skeletal muscle and the appearance of an amorphous substance, primarily composed of cellular debris, were noted after 24 hours. The presence of neuwiedase at the injection site (detected by immunocytochemistry) revealed highly specific labeling of myofibril components of damaged myocytes. In addition, proteolysis of muscle proteins assayed through myofibrils extracted from gastrocnemius muscle indicated that neuwiedase provoked degradation of myofibrils, especially myosin. These results suggest that skeletal muscle damage, induced by neuwiedase, is probably due to its proteolytic action on myofibrils, which are responsible for the maintenance of the cellular architecture.

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“…It has been demonstrated that SVMPs are involved in the pathogenesis of local inflammation by inducing edema and releasing matrix metalloproteases and inflammatory cytokines (14,24). Our data demonstrate increased levels of IL-8 in the supernatant of rACLF-treated cells.…”

Section: Discussionmentioning

confidence: 99%

“…SVMPs are responsible for most of the local and systemic effects observed during envenomation such as hemorrhage, skin lesions, necrosis, inflammation and cytokine-dependent inflammatory cells influx (4,11,13,14). SVMPs are divided into four groups (PI, II, III and IV) and subgroups that differ for the presence of additional domains on the carboxyl side of the metalloproteinase domain (4,12,17).…”

Section: Introductionmentioning

confidence: 99%

“…SVMPs are divided into four groups (PI, II, III and IV) and subgroups that differ for the presence of additional domains on the carboxyl side of the metalloproteinase domain (4,12,17). SVMPs display several activities such as degradation of blood coagulating factors (7,30), extracellular matrix (ECM) components (4,14,23,26) and cell receptors such as the cell-surface β1-integrin receptor (18). Besides these activities, SVMPs were reported to induce apoptosis in endothelial cells (2,32).…”

Section: Introductionmentioning

confidence: 99%

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rACLF, a recombinant snake venom metalloprotease, activates endothelial cells in vitro

Moraes¹,

Fritzen²,

Chudzinski-Tavassi³

et al. 2008

J. Venom. Anim. Toxins incl. Trop. Dis

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Snake venom metalloproteinases:Their role in the pathogenesis of local tissue damage

Cited by 513 publications

References 84 publications

Role of nitric oxide in the local and systemic pathophysiological effects induced by Bothrops asper snake venom in mice

Role of nitric oxide in the local and systemic pathophysiological effects induced by Bothrops asper snake venom in mice

Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study

rACLF, a recombinant snake venom metalloprotease, activates endothelial cells in vitro

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