SNAC4 and SNAC9 transcription factors show contrasting effects on tomato carotenoids biosynthesis and softening

Kou, Xiaohong; Zhao, Yanan; Wu, Caie; Jiang, Bianling; Zhang, Zhi; Rathbun, Jacob Robert; He, Yulong; Xue, Zhaohui

doi:10.1016/j.postharvbio.2018.05.008

Cited by 45 publications

(38 citation statements)

References 46 publications

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“…The expression level of SmNAC69 first showed a decreasing trend and then gradually recovered to the normal expression level, which is similar to the reported expression trend of SlNAC9 under PEG drought stress (Kou et al, 2017). Subsequent studies in the tomato NAC family have confirmed that the SlNAC9 gene is involved in carotene synthesis and fruit softening of tomato (Kou et al, 2018). Whether SmNAC69 has the same biological function is still unknown and hence it is necessary to verify the specific function and mechanism of this gene in future research using either transgenic or gene silencing technology.…”

Section: Regulation Of Nac Transcription Factors Under Abiotic Stresssupporting

confidence: 81%

Transcriptome-wide and expression analysis of the NAC gene family in pepino (Solanum muricatum) during drought stress

Yang

Zhu

Huang

et al. 2021

PeerJ

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Solanum muricatum (Pepino) is an increasingly popular solanaceous crop and is tolerant of drought conditions. In this study, 71 NAC transcription factor family genes of S. muricatum were selected to provide a theoretical basis for subsequent in-depth study of their regulatory roles in the response to biological and abiotic stresses, and were subjected to whole-genome analysis. The NAC sequences obtained by transcriptome sequencing were subjected to bioinformatics prediction and analysis. Three concentration gradient drought stresses were applied to the plants, and the target gene sequences were analyzed by qPCR to determine their expression under drought stress. The results showed that the S. muricatum NAC family contains 71 genes, 47 of which have conserved domains. The protein sequence length, molecular weight, hydrophilicity, aliphatic index and isoelectric point of these transcription factors were predicted and analyzed. Phylogenetic analysis showed that the S. muricatum NAC gene family is divided into seven subfamilies. Some NAC genes of S. muricatum are closely related to the NAC genes of Solanaceae crops such as tomato, pepper and potato. The seedlings of S. muricatum were grown under different gradients of drought stress conditions and qPCR was used to analyze the NAC expression in roots, stems, leaves and flowers. The results showed that 13 genes did not respond to drought stress while 58 NAC genes of S. muricatum that responded to drought stress had obvious tissue expression specificity. The overall expression levels in the root were found to be high. The number of genes at extremely significant expression levels was very large, with significant polarization. Seven NAC genes with significant responses were selected to analyze their expression trend in the different drought stress gradients. It was found that genes with the same expression trend also had the same or part of the same conserved domain. Seven SmNACs that may play an important role in drought stress were selected for NAC amino acid sequence alignment of Solanaceae crops. Four had strong similarity to other Solanaceae NAC amino acid sequences, and SmNAC has high homology with the Solanum pennellii. The NAC transcription factor family genes of S. muricatum showed strong structural conservation. Under drought stress, the expression of NAC transcription factor family genes of S. muricatum changed significantly, which actively responded to and participated in the regulation process of drought stress, thereby laying foundations for subsequent in-depth research of the specific functions of NAC transcription factor family genes of S. muricatum.

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Section: Regulation Of Nac Transcription Factors Under Abiotic Stresssupporting

confidence: 81%

Transcriptome-wide and expression analysis of the NAC gene family in pepino (Solanum muricatum) during drought stress

Yang

Zhu

Huang

et al. 2021

PeerJ

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“…On the other hand, delayed ripening was also observed in SlCMB1 -downregulated tomato fruit, SlACS2 , SlACS4 , SlACO1 , and SlACO3 were repressed, and ethylene production and carotenoid content were all reduced 40 . Downregulation of SlNAC48 and SlNAC19 inhibits fruit ripening, and SlACS2 , SlACS4 , and SlACO1 are seriously repressed 41 . These studies indicated that the effect of ripening regulators from different transcription factor families on fruit ripening may occur through the regulation of ethylene biosynthesis genes.…”

Section: Discussionmentioning

confidence: 99%

SlGRAS4 accelerates fruit ripening by regulating ethylene biosynthesis genes and SlMADS1 in tomato

Liu

Shi

et al. 2021

Hortic Res

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GRAS proteins are plant-specific transcription factors that play crucial roles in plant development and stress responses. However, their involvement in the ripening of economically important fruits and their transcriptional regulatory mechanisms remain largely unclear. Here, we demonstrated that SlGRAS4, encoding a transcription factor of the GRAS family, was induced by the tomato ripening process and regulated by ethylene. Overexpression of SlGRAS4 accelerated fruit ripening, increased the total carotenoid content and increased PSY1 expression in SlGRAS4-OE fruit compared to wild-type fruit. The expression levels of key ethylene biosynthesis genes (SlACS2, SlACS4, SlACO1, and SlACO3) and crucial ripening regulators (RIN and NOR) were increased in SlGRAS4-OE fruit. The negative regulator of tomato fruit ripening, SlMADS1, was repressed in OE fruit. Exogenous ethylene and 1-MCP treatment revealed that more endogenous ethylene was derived in SlGRAS4-OE fruit. More obvious phenotypes were observed in OE seedlings after ACC treatment. Yeast one-hybrid and dual-luciferase assays confirmed that SlGRAS4 can directly bind SlACO1 and SlACO3 promoters to activate their transcription, and SlGRAS4 can also directly repress SlMADS1 expression. Our study identified that SlGRAS4 acts as a new regulator of fruit ripening by regulating ethylene biosynthesis genes in a direct manner. This provides new knowledge of GRAS transcription factors involved in regulating fruit ripening.

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“…In tomato, over-expression of SlNAC1 leads to a decline in the contents of carotenoid and ABA, decreasing the synthesis of ethylene [62]. In addition, the SNAC4 (the protein structure of SNAC4 is very similar to that of ANAC072) and SNAC9 positively and negatively regulate fruit ripening and carotenoid accumulation by affecting ethylene and ABA levels [64]. Our results indicated that pummelo homologs of NAC072 (c13516.graph_c0) and NAC74 (c9680.graph_c0) are negatively and positively correlated with genes involved in carotenoid biosynthesis, respectively (Table S6 (Supplementary Materials); Figure 8E,K).…”

Section: Discussionmentioning

confidence: 99%

Illumina® Sequencing Reveals Candidate Genes of Carotenoid Metabolism in Three Pummelo Cultivars (Citrus Maxima) with Different Pulp Color

Jiang

Zhang

Lin

et al. 2019

IJMS

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Pummelo (Citrus maxima) is one of important fruit trees, which belongs to Citrus species. The fruits of different pummelo cultivars have different colors and differ in the contents of carotenoid. Our results clearly showed that ‘Huangjinmiyou’ (HJMY) has the highest content of β-carotene, followed by ‘Hongroumiyou’ (HRMY) and ‘Guanximiyou’ (GXMY). Lycopene is dominantly accumulated in HRMY. However, the molecular mechanism underlying the carotenoid accumulation in pummelo flesh is not fully understood. In this study, we used the RNA-Seq technique to investigate the candidate genes of carotenoid metabolism in the flesh of pummelo cv. GXMY and its mutants HRMY and HJMY in three development periods of fruit. After data assembly and bioinformatic analysis, a total of 357 genes involved in biosynthesis of secondary metabolites were isolated, of which 12 differentially expressed genes (DEGs) are involved in carotenoid biosynthesis. Among these 12 DEGs, phytoene synthase (PSY2), lycopene β-cyclase (LYCB2), lycopene Ɛ-cyclase (LYCE), carotenoid cleavage dioxygenases (CCD4), 9-cis-epoxycarotenoid dioxygenase (NCED2), aldehyde oxidase 3 (AAO3), and ABA 8′-hydroxylases (CYP707A1) are the most distinct DEGs in three pummelo cultivars. The co-expression analysis revealed that the expression patterns of several transcription factors such as bHLH, MYB, ERF, NAC and WRKY are highly correlated with DEGs, which are involved in carotenoid biosynthesis. In addition, the expression patterns of 22 DEGs were validated by real-time quantitative PCR (RT-qPCR) and the results are highly concordant with the RNA-Seq results. Our results provide a global vision of transcriptomic profile among three pummelo cultivars with different pulp colors. These results would be beneficial to further study the molecular mechanism of carotenoid accumulation in pummelo flesh and help the breeding of citrus with high carotenoid content.

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SNAC4 and SNAC9 transcription factors show contrasting effects on tomato carotenoids biosynthesis and softening

Cited by 45 publications

References 46 publications

Transcriptome-wide and expression analysis of the NAC gene family in pepino (Solanum muricatum) during drought stress

Transcriptome-wide and expression analysis of the NAC gene family in pepino (Solanum muricatum) during drought stress

SlGRAS4 accelerates fruit ripening by regulating ethylene biosynthesis genes and SlMADS1 in tomato

Illumina® Sequencing Reveals Candidate Genes of Carotenoid Metabolism in Three Pummelo Cultivars (Citrus Maxima) with Different Pulp Color

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