Smut disease assessment by PCR and microscopy in inoculated tissue cultured sugarcane cultivars

Singh, Nisha; Somai, Benesh Munilal; Pillay, Daisy

doi:10.1016/j.plantsci.2004.05.006

Cited by 51 publications

(46 citation statements)

References 15 publications

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“…All DNA samples isolated from the mycelia of putative S. scitamineum were subjected to PCR detection to verify S. scitamineum using primers bE4 and bE8, as described by Singh et al (2004). Ten microliters from each PCR were subjected to electrophoresis, stained, and photographed under ultraviolet light.…”

Section: Pcr Identification Of Pathogen Isolatesmentioning

confidence: 99%

“…With the rapid development of molecular biology, several methods have been found to be useful for analyzing phylogenetic relationships and genetic variations in fungi (Henson, 1992;Pagel, 1999;Thomas and Richard, 2004). Molecular detection of the smut pathogen in sugarcane has become possible by using polymerase chain reaction (PCR) to amplify the bE mating-type gene of S. scitamineum (Byther and Steiner, 1974;Albert and Schenck, 1996;Schenck, 1998;Singh et al, 2004;Su et al, 2013). Random-amplified polymorphic DNA, amplified fragment length polymorphism, sequence-related amplified polymorphism, inter-simple sequence repeat, and internal transcribed spacer (ITS) sequence analysis have been used to evaluate intraspecies diversity within S. scitamineum isolates (Victoria et al, 1997;Bakkeren et al, 2000;Braithwaite et al, 2004;Stoll et al, 2003Stoll et al, , 2005Xu et al, 2004Xu et al, , 2014Singh et al, 2005;Baraket et al, 2009;Que et al, 2012Que et al, , 2014a.…”

Section: Introductionmentioning

confidence: 99%

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Molecular variation of Sporisorium scitamineum in Mainland China revealed by internal transcribed spacers

et al. 2015

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ABSTRACT. Sugarcane smut caused by the fungus Sporisorium scitamineum is a worldwide disease and also one of the most prevalent diseases in sugarcane production in mainland China. To study molecular variation in S. scitamineum, 23 S. scitamineum isolates from the 6 primary sugarcane production areas in mainland, China (Guangxi, Yunnan, Guangdong, Hainan, Fujian, and Jiangxi Provinces), were assessed using internal transcribed spacer (ITS) methods. The results of ITS sequence analysis showed that the organisms can be defined at the genus level, including Ustilago and Sporisorium, and can also differentiate between closely related species. This method was not suitable for phylogenetic relationship analysis of different S. scitamineum isolates and could not provide support regarding their race ascription at the molecular level. The results of the present study will be useful for studies examining the molecular diversity of S. scita-7895 Molecular variation of Sporisorium scitamineum ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 7894-7909 (2015) mineum and for establishing a genetic foundation for their pathogenicity differentiation and new race detection. In addition, our results can provide useful information for the pathogen selection principle in sugarcane smut resistance breeding and variety distribution.

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Section: Pcr Identification Of Pathogen Isolatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular variation of Sporisorium scitamineum in Mainland China revealed by internal transcribed spacers

et al. 2015

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“…Sugarcane smut was first reported from Natal in South Africa (McMartin 1945). The disease has been associated with specific problems in sugarcaneproducing areas of the world (Singh et al 2004), affecting both plant growth and juice quality (Martı´nez et al 2000). The fungus is spread from plant to plant by airborne teliospores.…”

Section: Introductionmentioning

confidence: 99%

“…At the teliospore germination time of S. scitamineum, spores extend in size, split its exosporidium (external wall of teliospores) and produce a probasidium from which, following meiosis, four sporidia emerge. Haploid sporidia represent the second cell type (Singh et al 2004). Germination of sporidia leads to the production of a germ tube or same sporidial cells (Chona 1943).…”

Section: Introductionmentioning

confidence: 99%

“…Although CA produced inhibition of all the cell types, it seems that these cells were able to use CA as a carbon source since higher times of incubation produced less inhibition (Figure 3). With the extension of hyphal growth around the vascular tissues, there is the possibility that S. scitaminea spread quickly and systemically into the plant through the vascular system (Singh et al 2004). However, if sugarcane is able to inhibit dycaryotic mycelium formation, infectivity would be reduced.…”

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confidence: 99%

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In vitroeffects of caffeic acid upon growth of the fungiSporisorium scitamineum

Santiago

Armas

Bałanda

et al. 2010

Journal of Plant Interactions

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Caffeic acid (CA) sometimes behaves as a potent phytotoxin affecting plant and fungi growth and physiology. The aim of the present study was to investigate whether CA at a concentration range similar to that found in sugarcane leaves, had any effect against different phases of Sporisorium scitamineum growth cycle. Leaf CA concentration from two different sugarcane cultivars, Mayari (My) 55-14, resistant, and Barbados (B) 42231, susceptible to smut, was chromatographically quantified by HPLC. A smut elicitor promoted an increase of CA concentration in the resistant cv. while no effect was produced in the susceptible one. The effect of CA upon S. scitamineum growth cycle showed to be dependent of both concentration and time. At 5.0 mg ml (1 , CA produced and inhibition of teliospore germination, haploid sporidia production and dikaryotic mycelium appearance. At 30 mg ml (1 , CA produced similar effects to these just described. Inhibition was more evident after 24 h or 28 h incubation of teliospores in CA solution than after 48 h. CA at 20 mg ml (1 reduced both germination of teliospores and production of haploid sporidia but it had no significant effect on dikaryotic mycelium appearance after 24-h incubation.

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Changes in soluble and cell wall-bound hydroxycinnamic and hydroxybenzoic acids in sugarcane cultivars inoculated with Sporisorium scitamineum sporidia

et al. 2009

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The accumulation of soluble and cell wallbound phenolics in the sugarcane stems of young plants from highly resistant cv. My 5514 and susceptible cv. B 42231, inoculated or not inoculated with smut sporidia, was studied. The ratio of inoculated to uninoculated plants of some cell wallbound phenolics, such as ferulic, caffeic, and syringic acids increased for the resistant cv. My 5514, whereas it was maintained more or less constantly for the susceptible cv. B 42231. The highest increase of this ratio in the resistant cv. My 5514 corresponded to both caffeic and syringic acids. This could result in a better capacity to cv. My 5514 for an increase in the frequency of bridges between lignin fragments through ester-ether linkages for reinforcing the cell wall and major resistance to the disease. This reinforcement of the cell wall could provide an effective barrier to pathogen entry and spread. Soluble sub-fractions of all phenolics detected showed non-stable patterns. Caffeic acid, that regulates phenylalanine ammonia-lyase activity in sugarcane, showed a significant decrease in its titre at 24 h in the resistant cultivar, principally in the free soluble fraction, whilst the susceptible cultivar enhanced it. We hypothesise that the pathway of hydroxybenzoic acids is only activated once the level of p-coumaric acid justifies the accumulation of hydroxycinnamic acids required for reinforcing the cell wall after inoculation.

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Smut disease assessment by PCR and microscopy in inoculated tissue cultured sugarcane cultivars

Cited by 51 publications

References 15 publications

Molecular variation of Sporisorium scitamineum in Mainland China revealed by internal transcribed spacers

Molecular variation of Sporisorium scitamineum in Mainland China revealed by internal transcribed spacers

In vitroeffects of caffeic acid upon growth of the fungiSporisorium scitamineum

Changes in soluble and cell wall-bound hydroxycinnamic and hydroxybenzoic acids in sugarcane cultivars inoculated with Sporisorium scitamineum sporidia

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