Molecular variation of Sporisorium scitamineum in Mainland China revealed by internal transcribed spacers

Abstract

ABSTRACT. Sugarcane smut caused by the fungus Sporisorium scitamineum is a worldwide disease and also one of the most prevalent diseases in sugarcane production in mainland China. To study molecular variation in S. scitamineum, 23 S. scitamineum isolates from the 6 primary sugarcane production areas in mainland, China (Guangxi, Yunnan, Guangdong, Hainan, Fujian, and Jiangxi Provinces), were assessed using internal transcribed spacer (ITS) methods. The results of ITS sequence analysis showed that the organisms can be defined at the genus level, including Ustilago and Sporisorium, and can also differentiate between closely related species. This method was not suitable for phylogenetic relationship analysis of different S. scitamineum isolates and could not provide support regarding their race ascription at the molecular level. The results of the present study will be useful for studies examining the molecular diversity of S. scita-7895 Molecular variation of Sporisorium scitamineum ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 7894-7909 (2015) mineum and for establishing a genetic foundation for their pathogenicity differentiation and new race detection. In addition, our results can provide useful information for the pathogen selection principle in sugarcane smut resistance breeding and variety distribution.

Molecular variability among Brazilian strains of the sugarcane smut pathogen and the genetic basis of host specialization in smut fungi

“…The ITS sequencing data was even more inefficient, producing identical sequences for all strains, including those from Argentina. The ITS data produced by other authors revealed some variability in isolates from different countries (Singh et al, 2005;Zhang et al, 2015). After align our data with other 30 ITS sequences available at genbank, the Brazilian and Argentine haplotypes were equal to the most common haplotype worldwide (Fig.…”

“…Many genetic studies have been conducted on global and local levels, using different strategies to assess S. scitamineum molecular variability and virulence degree (Bhuiyan et al, 2015;Fattah et al, 2010;Luzaran et al, 2012;Que et al, 2012;Raboin et al, 2007;Rago et al, 2009;Singh et al, 2005;Xu et al, 2004Xu et al, , 2014Zhang et al, 2015). In an overall view, low levels of genetic variation were found in American and African isolates while high levels were found in Southeast Asian populations.…”

Identification of Genomic Regions Controlling Leaf Scald Resistance in Sugarcane Using a Bi-parental Mapping Population and Selective Genotyping by Sequencing

“…Similarly, the number of LGs in the susceptible parent L 99-226 (137) was expectedly more than the ideally expected number 120. Such genetic map fragmentation in L99-226 could be due to a lower number of polymorphic markers due to inbreeding-caused reduced heterozygosity in modern sugarcane ( Deren, 1995 ), and low marker density and uneven distribution of linked SD markers. In addition, the high number of recombinant linkage groups in the present study could be attributed to the small mapping population size.…”

The Morphological And Molecular Variability Of Sporisorium Scitamineum Isolates In Eswatini

“…The quality and concentration of DNA was analysed by 1% agarose gel electrophoresis and a nanodrop spectrophotometer. To verify the identity of the fungus, the DNA that was extracted from the samples was ampli ed on conventional PCR using the bE4 (5'-CGCTCTGGTTCATCAACG − 3') and bE8 (5'-TGCTGTCGATGGAAGGTGT − 3') primers that are speci c for S. scitamineum (Zhang et al, 2015).…”

“…The DNA was sent for sequencing at Inqaba Biotech, South Africa. Sequencing was based on the ITS region of the fungal genomic DNA using the ITS1 (5'-TCCGTAGGTGAACCTGCGG − 3') and ITS4 (5' -TCCTCCGCTTATTGATATGC − 3') primer pair (White et al, 1990;Zhang et al, 2015). The ITS target region was ampli ed using OneTaq® Quick-Load® 2X Master Mix (NEB, Catalogue No.…”