Smartphone-based colorimetric detection of cardiac troponin T via label-free aptasensing

Optical Nanobiosensor-Based Point-of-Care Testing for Cardiovascular Disease Biomarkers

“…Recently, different types of nanomaterial-based PoCT devices have been developed and utilized various optical read-out techniques: Ps-AuNPs-Aptamer-BSA microparticles label-free microfluidic paper-based colorimetric sensing device (μ-PAD) for cTnT via smartphone-based image analysis; red fluorescent-based PoC LFIA systems for the detection of cTnI; QBs@SiO 2 -COOH/ quantum-dot nanobeads LFIA colorimetric assay system for cTnI; functionalized gold chip-based PoC-SPR biosensor for the detection of NT-proBNP; Nile blue tagged Ag/AuNPs core/shell-based SERS LFA strip for multiplex detection of cardiac biomarkers of Myo, cTnI, and CK-MB in real samples; malachite green isothiocyanate tagged with AuNPs@anti-cTnI and Nile blue tagged with AuNPs@anti-MB immunoassays-based finger-pump microfluidic chip for simultaneous SERS detection of cTnI and CK-MB …”

“…Stanciu et al fabricated a label-free microfluidic paper-based colorimetric sensing device (μ-PAD) for cTnT via smartphonebased image analysis. 232 A microfluid wax-printed paper device was designed with six testing areas: two target-testing areas, two as negative control areas, and another two as reference areas, as shown in Figure 11b(i). The proposed cTnT detection was the interaction between Ps-AuNPs-Aptamer-BSA microparticles with aptamer-cTnT, which induced aggregation and produced a color change from pink to purple (Figure 11b(ii)).…”

“…(b) Design of microfluidic paper-based colorimetric sensing device (i) and IL-6 detection principles (ii). Reprinted with permission from ref . Copyright 2023 Elsevier.…”

“…Stanciu et al fabricated a label-free microfluidic paper-based colorimetric sensing device (μ-PAD) for cTnT via smartphone-based image analysis . A microfluid wax-printed paper device was designed with six testing areas: two target-testing areas, two as negative control areas, and another two as reference areas, as shown in Figure b­(i).…”

Multiplexed aptasensor for detection of acute myocardial infraction (AMI) biomarkers

“…[20][21][22] Alternatively, running multiple tests in series with a handheld analyzer, or by running multiple tests in parallel on a benchtop system can improve the accuracy and efficiency, optimizing the cost, inventory, and/or patient comfort. [23][24][25][26] Aptamers, consisting of peptide molecules or short synthetic oligonucleotides, haven't been explored for AMI diagnosis intensively. Aptamers are designed to be the specific recognition units for cardiac biomarkers while staying stable over a range of temperatures, pH values, and storage conditions.…”