Smart Hydrogel Microfluidics for Single‐Cell Multiplexed Secretomic Analysis with High Sensitivity

Hsu, Myat Noe; Wei, Shih-Chung; Guo, Song; Phan, Dinh‐Tuan; Zhang, Yong; Chen, Chia-Hung

doi:10.1002/smll.201802918

Cited by 56 publications

(36 citation statements)

References 43 publications

(51 reference statements)

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“…This observation suggests that Q c had no significant impact on the diameter of droplets that were produced in the step. However, the value of Q c could influence the diameter of droplets, which were generated in the flow-focusing nozzle, and by increasing Q c , the diameter of generated droplets decreased as it could be expected 27 , 29 , 30 .…”

Section: Resultsmentioning

confidence: 98%

Microfluidic on-chip production of microgels using combined geometries

Shieh

Saadatmand

Eskandari

et al. 2021

Sci Rep

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Microfluidic on-chip production of microgels using external gelation can serve numerous applications that involve encapsulation of sensitive cargos. Nevertheless, on-chip production of microgels in microfluidic devices can be challenging due to problems induced by the rapid increase in precursor solution viscosity like clogging. Here, a novel design incorporating a step, which includes a sudden increase in cross-sectional area, before a flow-focusing nozzle was proposed for microfluidic droplet generators. Besides, a shielding oil phase was utilized to avoid the occurrence of emulsification and gelation stages simultaneously. The step which was located before the flow-focusing nozzle facilitated the full shielding of the dispersed phase due to 3-dimensional fluid flow in this geometry. The results showed that the microfluidic device was capable of generating highly monodispersed spherical droplets (CV < 2% for step and CV < 5% for flow-focusing nozzle) with an average diameter in the range of 90–190 μm, both in step and flow-focusing nozzle. Moreover, it was proved that the device could adequately create a shelter for the dispersed phase regardless of the droplet formation locus. The ability of this microfluidic device in the production of microgels was validated by creating alginate microgels (with an average diameter of ~ 100 μm) through an external gelation process with on-chip calcium chloride emulsion in mineral oil.

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Section: Resultsmentioning

confidence: 98%

Microfluidic on-chip production of microgels using combined geometries

Shieh

Saadatmand

Eskandari

et al. 2021

Sci Rep

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show abstract

“…It has a potential to quantify and characterize the enzymes that secreted from single cell but it also required creating small-size droplets for single cell-based absolute quantification of enzyme [79]. The use of hydrogel immunosensors is another approach for single-cell multiplexed secretion analysis that has been proven recently [80]. The main approach to study enzymes is probably directed evolution assays.…”

Section: Droplet-based Microfluidics For Proteomicmentioning

confidence: 99%

Recent Advances in Droplet-based Microfluidic Technologies for Biochemistry and Molecular Biology

2019

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Recently, droplet-based microfluidic systems have been widely used in various biochemical and molecular biological assays. Since this platform technique allows manipulation of large amounts of data and also provides absolute accuracy in comparison to conventional bioanalytical approaches, over the last decade a range of basic biochemical and molecular biological operations have been transferred to drop-based microfluidic formats. In this review, we introduce recent advances and examples of droplet-based microfluidic techniques that have been applied in biochemistry and molecular biology research including genomics, proteomics and cellomics. Their advantages and weaknesses in various applications are also comprehensively discussed here. The purpose of this review is to provide a new point of view and current status in droplet-based microfluidics to biochemists and molecular biologists. We hope that this review will accelerate communications between researchers who are working in droplet-based microfluidics, biochemistry and molecular biology.

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Section: Immunodiagnosis Studies On Microfluidic Chipsmentioning

confidence: 99%

“…For example, Revzin and co‐workers detected T‐cell secreted cytokines in a glass slide which were coated by patterned a polyethylene glycol (PEG) layer. Moreover, hydrogel can also be used for co‐encapsulation with single cells by emulsification (Figure D) . It works as particle immunosensors in the culture medium droplets to capture secreted cytokines for high‐resolution secretion detection.…”

Section: Immunodiagnosis Studies On Microfluidic Chipsmentioning

confidence: 99%

Analysis of Leukocyte Behaviors on Microfluidic Chips

Yang

Cao

2019

Adv Healthcare Materials

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The orchestration of massive leukocytes in the immune system protects humans from invading pathogens and abnormal cells in the body. So far, researches focusing on leukocyte behaviors are performed based on both in vivo and in vitro models. The in vivo animal models are usually less controllable due to their extreme complexity and nonignorable species issue. Therefore, many researchers turn to in vitro models. With the advances in micro/nanofabrication, the microfluidic chip has emerged as a novel platform for model construction in multiple biomedical research fields. Specifically, the microfluidic chip is used to study leukocyte behaviors, due to its incomparable advantages in high throughput, precise control, and flexible integration. Moreover, the small size of the microstructures on the microfluidic chip can better mimic the native microenvironment of leukocytes, which contributes to a more reliable recapitulation. Herein are reviewed the recent advances in microfluidic chip–based leukocyte behavior analysis to provide an overview of this field. Detailed discussions are specifically focused on host defense against pathogens, immunodiagnosis, and immunotherapy studies on microfluidic chips. Finally, the current technical challenges are discussed, as well as possible innovations in this field to improve the related applications.

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Smart Hydrogel Microfluidics for Single‐Cell Multiplexed Secretomic Analysis with High Sensitivity

Cited by 56 publications

References 43 publications

Microfluidic on-chip production of microgels using combined geometries

Microfluidic on-chip production of microgels using combined geometries

Recent Advances in Droplet-based Microfluidic Technologies for Biochemistry and Molecular Biology

Analysis of Leukocyte Behaviors on Microfluidic Chips

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