Small, smaller, smallest: Miniaturization of chromatographic process development

Silva, Tiago Castanheira; Eppink, M.H.M.; Ottens, Marcel

doi:10.1016/j.chroma.2022.463451

Cited by 7 publications

(5 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several techniques have been suggested for the fractionation of biopolymers, such as precipitation, [7][8][9] membrane processes, 10,11 and liquid chromatography, 12,13 among others. However, specifically for fractionation of biopolymers with limited value, these approaches have some disadvantages.…”

Section: Introductionmentioning

confidence: 99%

“…Even though chromatography presents high purification factors, it is typically expensive due to low throughput and complex scale-up. [13][14][15] In addition, membrane separations for the highly viscous streams will result in a very low throughput. An effective downstream process for biopolymers requires low cost and complexity, along with high yields and purity.…”

Section: Introductionmentioning

confidence: 99%

“…For the precipitation, only a single fraction is recovered, and the process requires large amounts of organic solvent. Even though chromatography presents high purification factors, it is typically expensive due to low throughput and complex scale‐up 13–15 . In addition, membrane separations for the highly viscous streams will result in a very low throughput.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Polymer and alcohol‐based three‐phase partitioning systems for separation of polysaccharide and protein

Antunes,

Temmink,

Schuur

2023

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BackgroundNatural polymers are macromolecules produced by living organisms, with a wide range of applications and relevance for the development of a circular economy. However, large‐scale production is still hindered by several factors, such as downstream processing. In this work, three‐phase partitioning (TPP) systems were investigated for separation of model polysaccharide (dextran, alginate and gum arabic) from protein (Bovine serum albumin (BSA) and lysozyme). The recyclability of phase‐forming compounds used to form the extractive platform was assessed by ultrafiltration (UF). This study contributes to development of production processes for biopolymers from fermented waste by proposing an effective separation technique for fractionation of biopolymers. Previously such biopolymers were only collected as mixtures, and with the studied approaches, also fractionation may be applied of polysaccharides from proteins. With the chosen systems, the scope of TPP systems is expanded by using another class of phase‐forming compound (polymers), and in addition, UF was studied as a versatile regeneration approach.ResultsWithin the TPP approach, the best separation of dextran from BSA was achieved using TPP systems composed of 25 wt% PEG + 25 wt% K3C6H5O7 and 36 wt% EtOH + 10 wt% K3PO4, in which more than 95% of dextran and BSA were found as precipitate and partitioned to top phase (PEG or EtOH‐rich), respectively. By using other model compounds, it was found that the molecular weight and charge of biopolymer play a key role in the yield and selectivity of TPP systems. Finally, by using ultrafiltration/diafiltration, about 99% of ethanol and phosphate salt used to form the extractive platform could be retrieved in the permeate stream.ConclusionThe high extraction yields, selectivity and recyclability of phase‐forming compounds confirm the potential of polymer‐based and alcohol‐based TPP systems to fractionate biopolymer mixtures.This article is protected by copyright. All rights reserved.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Polymer and alcohol‐based three‐phase partitioning systems for separation of polysaccharide and protein

Antunes,

Temmink,

Schuur

2023

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…Increasing throughput and speed of analytics is a vital part of improving efficiency. Analytics that can be paired with highthroughput screening (HTS) methods can enable faster development and identification of robust operating conditions (Bensch et al, 2005;Effio & Hubbuch, 2015;Keulen et al, 2022;Silva et al, 2022aSilva et al, , 2022b.…”

Section: Patient Safety and Process Robustnessmentioning

confidence: 99%

Status and future developments for downstream processing of biological products

Jungbauer,

Ferreira,

Butler

et al. 2024

Biotech & Bioengineering

View full text Add to dashboard Cite

Governments and biopharmaceutical organizations aggressively leveraged expeditious communication capabilities, decision models, and global strategies to make a COVID‐19 vaccine happen within a period of 12 months. This was an unusual effort and cannot be transferred to normal times. However, this focus on a single vaccine has also led to other treatments and drug developments being sidelined. Society expects the pharmaceutical industry to provide an uninterrupted supply of medicines. However, it is often overlooked how complex the manufacture of these compounds is and what logistics are required, not to mention the time needed to develop new drugs. The overarching theme, therefore, is patient access and how we can help ensure access and extend it to low‐ and middle‐income countries. Despite unceasing efforts to make medications available to all patient populations, this must never be done at the expense of patient safety. A major fraction of the costs in biopharmaceutical manufacturing are for drug discovery, process development, and clinical studies. Infrastructure costs are very difficult to quantify because they often depend on whether a greenfield facility or an existing, depreciated facility is used or adapted for a new product. To accelerate process development concepts of platform process and prior knowledge are increasingly leveraged. While more traditional protein therapeutics continue to dominate the field, we are also experiencing the exciting emergence and evolution of other therapeutic formats (bispecifics, tetravalent mAbs, antibody‐drug conjugates, enzymes, peptides, etc.) that offer unique treatment options for patients. Protein modalities are still dominant, but new modalities are being developed that can be learned from including advanced therapeutics‐like cell and gene therapies. The industry must develop a model‐based strategy for process development and technologies such as continuous integrated biomanufacturing must be adopted. The overall conclusion is that the pandemic pace was unsustainable, focused on vaccine delivery at the expense of other modalities/disease targets, and had implications for professional and personal life (work‐life balance). Routinely reducing development time from 10 years to 1 year is nearly impossible to achieve. Environmental aspects of sustainable downstream processing are also described.

show abstract

“…However, these methods require a significant amount of sample and experimental effort. Given that the samples usually investigated in chromatography separation are expensive (e.g., therapeutic proteins, peptides), novel approaches to determine sorption isotherm were developed, such as direct inverse method [9], microfluidic chip approach [10] and modern biosensors methods such as SPR and quartz crystal microbalance [11].…”

Section: Introductionmentioning

confidence: 99%

Metal-Chelating Peptides Separation Using Immobilized Metal Ion Affinity Chromatography: Experimental Methodology and Simulation

et al. 2022

View full text Add to dashboard Cite

Metal-Chelating Peptides (MCPs), obtained from protein hydrolysates, present various applications in the field of nutrition, pharmacy, cosmetic etc. The separation of MCPs from hydrolysates mixture is challenging, yet, techniques based on peptide-metal ion interactions such as Immobilized Metal Ion Affinity Chromatography (IMAC) seem to be efficient. However, separation processes are time consuming and expensive, therefore separation prediction using chromatography modelling and simulation should be necessary. Meanwhile, the obtention of sorption isotherm for chromatography modelling is a crucial step. Thus, Surface Plasmon Resonance (SPR), a biosensor method efficient to screen MCPs in hydrolysates and with similarities to IMAC might be a good option to acquire sorption isotherm. This review highlights IMAC experimental methodology to separate MCPs and how, IMAC chromatography can be modelled using transport dispersive model and input data obtained from SPR for peptides separation simulation.

show abstract

Small, smaller, smallest: Miniaturization of chromatographic process development

Cited by 7 publications

References 28 publications

Polymer and alcohol‐based three‐phase partitioning systems for separation of polysaccharide and protein

Polymer and alcohol‐based three‐phase partitioning systems for separation of polysaccharide and protein

Status and future developments for downstream processing of biological products

Metal-Chelating Peptides Separation Using Immobilized Metal Ion Affinity Chromatography: Experimental Methodology and Simulation

Contact Info

Product

Resources

About