Metal-Chelating Peptides Separation Using Immobilized Metal Ion Affinity Chromatography: Experimental Methodology and Simulation

Abstract

Metal-Chelating Peptides (MCPs), obtained from protein hydrolysates, present various applications in the field of nutrition, pharmacy, cosmetic etc. The separation of MCPs from hydrolysates mixture is challenging, yet, techniques based on peptide-metal ion interactions such as Immobilized Metal Ion Affinity Chromatography (IMAC) seem to be efficient. However, separation processes are time consuming and expensive, therefore separation prediction using chromatography modelling and simulation should be necessary. Meanwhile, the obtention of sorption isotherm for chromatography modelling is a crucial step. Thus, Surface Plasmon Resonance (SPR), a biosensor method efficient to screen MCPs in hydrolysates and with similarities to IMAC might be a good option to acquire sorption isotherm. This review highlights IMAC experimental methodology to separate MCPs and how, IMAC chromatography can be modelled using transport dispersive model and input data obtained from SPR for peptides separation simulation.

Screening, separation and identification of metal-chelating peptides for nutritional, cosmetics and pharmaceutical applications

“…7 In addition, there is analogy between IMAC and SPR since both technologies are based on peptide-metal ion interactions and present some similarities: the same metal can be chosen for immobilization (e.g., Ni 2+ ) and the same complexing agent like NTA can be used, supported by the matrix in both technologies. 20 This analogy between both techniques may lead to efficient prediction of MCP separation in IMAC using sorption isotherm parameters obtained by SPR. 209 To perform MCP separation simulation, pdepe solver (Matlab) could be used.…”

Preparation of Polyacrylonitrile-Based Immobilized Copper-Ion Affinity Membranes for Protein Adsorption

“…The metal ions immobilized on a solid carrier are usually first-row transition metals (Cu 2+ , Ni 2+ , Zn 2+ , Co 2+ , etc.) [ 5 , 9 , 13 , 14 , 15 , 16 ]. The vacant valence orbitals of a first-row transition metal provide excellent stability for the coordination compound formed.…”

“…Biomolecules containing specific amino acids are absorbed into the metal ion-immobilized solid carrier with specific affinity, and thus separation and purification can be carried out [ 1 , 2 , 3 ]. This method offers the advantages of high capacity, high recovery, complete regeneration and low cost [ 12 , 16 ].…”

“…Via replacing the chromatographic solid particles with film-type membranes, better features and performance could be achieved for so-called membrane chromatography or the adsorptive membrane process: simple design, low pressure drop, fast mass transfer, small process time and easy scale-up [ 3 , 4 , 5 , 6 , 12 , 14 , 15 , 16 , 17 , 18 , 19 ]. The membrane is proposed to take the position of solid particles, with the advantages of allowing solutes to enter the macropores of a thin film through convection; such an adsorption process could thus be conducted under lower pressure and with quicker mass transport, leading to lower energy consumption and less cost.…”

“…Prior to metal ion immobilization, the chelating agent needs to be bound to the membrane matrix. The common choices of chelating agents are multidentates, such as iminodiacetic acid, tridentate (IDA), nitrilotriacetic acid, tetradentate (NTA), and N,N,N-tris(carboxymethyl)ethylenediamine, pentadentate (TED) [ 1 , 5 , 13 , 14 , 16 , 32 , 33 ], as well as triazine dyes [ 3 , 5 , 34 , 35 , 36 ]. Nevertheless, the direct coupling of a chelating agent onto a polymeric membrane matrix is not usually easy.…”