Small-scale field tests with transgenic potato, cv. Bintje, to test resistance to primary and secondary infections with potato virus y

Malnoë, Pia; Farinelli, Laurent; Collet, Gérald F.; Reust, W.

doi:10.1007/bf00014670

Cited by 48 publications

(20 citation statements)

References 33 publications

(37 reference statements)

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“…Later on, the virus particles migrate down to the progeny tubers; this migration is called the translocation of the virus (Debokx 1964). The following year, seed potatoes harvested from the primary infected plant will give rise to infected plants, usually called secondary infected plants (Malnoe et al 1994).…”

Section: Introductionmentioning

confidence: 99%

The movement of potato virus Y (PVY) in the vascular system of potato plants

Dupuis

2016

Eur J Plant Pathol

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Potato virus Y (PVY) is responsible for major viral diseases in most potato seed areas. It is transmitted by aphids in a non-persistent manner, and it is spread in potato fields by the winged aphids flying from an infected source plant to a healthy one. Six different PVY strains groups affect potato crops: PVY C , PVY N , PVY O , PVY N:O , PVY NTN , and PVY N-Wi . Nowadays, PVY NTN and PVY N-Wi are the predominant strains in Europe and the USA. After the infection of the leaf and accumulation of the virus, the virus is translocated to the progeny tubers. It is known that PVY N is better translocated than PVY O , but little is known about the translocation of the other PVY strains. The translocation of PVY occurs faster in young plants than in old plants; this mature plant resistance is generally explained by a restriction of the cell-to-cell movement of the virus in the leaves. The mother tuber may play an important role in explaining mature plant resistance. PVY is able to pass from one stem to the other stems of the same plant through the vascular system of the mother tuber, but it is unknown whether this vascular link between stems is permanent during the whole life of the plant. Two greenhouse trials were set up to study the spread of PVY in the vascular system of the potato plant. The PVY-susceptible cultivar Charlotte was used for both trials. It was demonstrated that all stems growing from a PVY-infected tuber will become infected sooner or later, and that PVY N-Wi translocates more efficiently to progeny tubers than PVY NTN . It was also demonstrated that the progressive decay of the mother tuber in the soil reduces the possibility for virus particles to infect healthy stems through the vascular system of the mother tuber. This new element contributes to a better understanding of the mechanism of mature plant resistance.

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Section: Introductionmentioning

confidence: 99%

The movement of potato virus Y (PVY) in the vascular system of potato plants

Dupuis

2016

Eur J Plant Pathol

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“…The use of the viral coat protein (CP) as a transgene gave the most important results, with delay and attenuation of symptoms or complete immunity. Powell et al (1986) first introduced, in this way, resistance against Tobacco mosaic virus (TMV) in tobacco plants then many other researchers obtained several engineered crops for commercial cultivation such as tomato resistant to TMV, Tomato mosaic virus (ToMV) (Sanders et al, 1992), Cucumber mosaic virus (CMV) (Fuchs et al, 1996), potato resistant to Potato virus X (PVX) (Jongedijk et al 1992), Potato virus Y (PVY) (Malnoe et al, 1994) and Potato leafroll virus (PLRV) (Presting et al, 1995). Moreover tobacco containing the CP genes for three viruses, Tomato spotted wilt virus (TSWV), Tomato chlorotic spot virus (TCSV), and Groundnut ringspot virus (GRSV) developed resistance to all of them (Prins et al, 1995).…”

Section: Molecular Crop Protection Using Recombinant Dna Techniquesmentioning

confidence: 99%

DNA Sequencing and Crop Protection

Tedeschi¹

2012

DNA Sequencing - Methods and Applications

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“…This approach relies on differences in the percentage, mean, and/or variance of infected plants between two genetically distinct cultivars (Clough and Hamm 1995;Fuchs and Gonsalves 1995;Fuchs et al 1998aFuchs et al , b 1999Gonsalves et al 1992;Kanieswki et al 1990;Malnoe et al 1994;Steinlage et al 2002). It is powerful to evaluate the level of resistance, however, it does not provide information on the spatial relationship among test plants nor does it illustrate disease progress in space.…”

Section: Introductionmentioning

confidence: 98%

Comparative spatial spread overtime of Zucchini Yellow Mosaic Virus (ZYMV) and Watermelon Mosaic Virus (WMV) in fields of transgenic squash expressing the coat protein genes of ZYMV and WMV, and in fields of nontransgenic squash

Klas

Fuchs

2006

Transgenic Res

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The spatial and temporal patterns of aphid-vectored spread of Zucchini Yellow Mosaic Virus (ZYMV) and Watermelon Mosaic Virus (WMV) were monitored over two consecutive years in plantings of nontransgenic and transgenic squash ZW-20H (commercial cv. Freedom II) and ZW-20B, both expressing the coat protein genes of ZYMV and WMV. All test plants were surrounded by nontransgenic plants that were mechanically inoculated with ZYMV or WMV, and served as primary virus source. Across all trials, none of the transgenic plants exhibited systemic symptoms upon infection by ZYMV and WMV but a few of them developed localized chlorotic dots and/or blotches, and had low mixed infection rates [4% (6 of 139) of ZW-20H and 9% (13 of 139) of ZW-20B], as shown by ELISA. Geostatistical analysis of ELISA positive transgenic plants indicated, (i) a lack of spatial relationship on spread of ZYMV and WMV for ZW-20H with flat omnidirectional experimental semivariograms that fitted poorly theoretical models, and (ii) some extent of spatial dependence on ZYMV spread for ZW-20B with a well structured experimental semivariogram that fitted poorly theoretical models during the first but not the second growing season. In contrast, a strong spatial dependence on spread of ZYMV and WMV was found for nontransgenic plants, which developed severe systemic symptoms, had prevalent mixed infection rates (62%, 86 of 139), and well-defined omnidirectional experimental semivariograms that fitted a spherical model. Geostatistical data were sustained by virus transmission experiments with Myzus persicae in screenhouses, showing that commercial transgenic squash ZW-20H alter the dynamics of ZYMV and WMV epidemics by preventing secondary plant-to-plant spread.

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Small-scale field tests with transgenic potato, cv. Bintje, to test resistance to primary and secondary infections with potato virus y

Cited by 48 publications

References 33 publications

The movement of potato virus Y (PVY) in the vascular system of potato plants

The movement of potato virus Y (PVY) in the vascular system of potato plants

DNA Sequencing and Crop Protection

Comparative spatial spread overtime of Zucchini Yellow Mosaic Virus (ZYMV) and Watermelon Mosaic Virus (WMV) in fields of transgenic squash expressing the coat protein genes of ZYMV and WMV, and in fields of nontransgenic squash

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