Small clone dissemination of tmexCD1-toprJ1–carrying Klebsiella pneumoniae isolates in a chicken farm

Wang, Xiaoming; Sun, Naiyan; Liu, Xiao; Li, Fan; Sun, Jianzhong; Huang, Jinhu; Li, Ruichao; Wang, Liping

doi:10.1016/j.jgar.2022.02.012

Cited by 6 publications

(3 citation statements)

References 33 publications

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“…Of these studies, 37 (58.7%) assessed mcr genes in isolates recovered from 2016 and earlier (i.e., before the ban), while 26 (41.3%) probed genes in isolates recovered from 2017 onwards (i.e., during the enforcement of the ban) (Table S1), suggesting that there are more publications on the incidence of mcr in Chinese poultry before than during the ban enforcement. However, some studies assayed mcr in isolates obtained during a period that spanned before and during the ban [63,85,133,135,157,162].…”

Section: Eastern Asia Chinamentioning

confidence: 99%

“…The E. fergusonii isolates coexpressed mcr with 43 additional resistance genes (including ESBL genes-bla OXA-10 and fosA) conferring resistance to 10 antimicrobial classes [137,138,163]. Klebsiella isolates contained >40 additional resistance genes (including armA, encoding 16S rRNA-methylase, conferring high-grade aminoglycoside resistance; ESBL; carbapenemasebla NDM-5 ; fosA; and PMQR genes) conferring resistance to eight antimicrobial classes [49,133,135,167]; Cronobacter isolates harboured mcr and 6 additional resistance genes (including PMQR; carbapenemase-bla NDM-9 ; ESBL-bla CTX-M-55/65 ; and fosA genes) conferring resistance to four antimicrobial classes [158]; and Aeromonas isolates contained mcr and 21 extra resistance genes (including PMQR, aac(6 )-Ib-cr, and ESBL genes) conferring resistance to eight antimicrobial classes [168,235], while 33 other resistance genes (including ESBL and PMQR genes) also conferring resistance to eight antimicrobial classes were coexpressed with mcr by Rauoltella isolates [86] (Table S1). Thus, the Chinese poultry sector constitutes a reservoir of multi-to pandrug-resistant organisms.…”

Section: Eastern Asia Chinamentioning

confidence: 99%

“…Most worrisome is that some Enterobacterales coexpressed mcr with plasmid-mediated transmissible genes tet(X) and tmexCD-toprJ, conferring high-level tigecycline resistance. E. coli isolates from pigeons coexpressed mcr-1 with tet(X4) and bla NDM-1 /bla NDM5 [92], while K. pneumoniae isolates from chickens contained mcr-8, mcr-10, tmexCD1-toprJ1, and bla CTX-M-27 [133,151,166,167]. This suggests that the Chinese poultry sector is a potential reservoir for the emergence and spread of pandrug-resistant organisms, posing emergent challenges to antimicrobial therapy.…”

Section: Eastern Asia Chinamentioning

confidence: 99%

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Mobile Colistin Resistance (mcr) Gene-Containing Organisms in Poultry Sector in Low- and Middle-Income Countries: Epidemiology, Characteristics, and One Health Control Strategies

et al. 2023

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Mobile colistin resistance (mcr) genes (mcr-1 to mcr-10) are plasmid-encoded genes that threaten the clinical utility of colistin (COL), one of the highest-priority critically important antibiotics (HP-CIAs) used to treat infections caused by multidrug-resistant and extensively drug-resistant bacteria in humans and animals. For more than six decades, COL has been used largely unregulated in the poultry sector in low- and middle-income countries (LMICs), and this has led to the development/spread of mcr gene-containing bacteria (MGCB). The prevalence rates of mcr-positive organisms from the poultry sector in LMICs between January 1970 and May 2023 range between 0.51% and 58.8%. Through horizontal gene transfer, conjugative plasmids possessing insertion sequences (ISs) (especially ISApl1), transposons (predominantly Tn6330), and integrons have enhanced the spread of mcr-1, mcr-2, mcr-3, mcr-4, mcr-5, mcr-7, mcr-8, mcr-9, and mcr-10 in the poultry sector in LMICs. These genes are harboured by Escherichia, Klebsiella, Proteus, Salmonella, Cronobacter, Citrobacter, Enterobacter, Shigella, Providencia, Aeromonas, Raoultella, Pseudomonas, and Acinetobacter species, belonging to diverse clones. The mcr-1, mcr-3, and mcr-10 genes have also been integrated into the chromosomes of these bacteria and are mobilizable by ISs and integrative conjugative elements. These bacteria often coexpress mcr with virulence genes and other genes conferring resistance to HP-CIAs, such as extended-spectrum cephalosporins, carbapenems, fosfomycin, fluoroquinolone, and tigecycline. The transmission routes and dynamics of MGCB from the poultry sector in LMICs within the One Health triad include contact with poultry birds, feed/drinking water, manure, poultry farmers and their farm workwear, farming equipment, the consumption and sale of contaminated poultry meat/egg and associated products, etc. The use of pre/probiotics and other non-antimicrobial alternatives in the raising of birds, the judicious use of non-critically important antibiotics for therapy, the banning of nontherapeutic COL use, improved vaccination, biosecurity, hand hygiene and sanitization, the development of rapid diagnostic test kits, and the intensified surveillance of mcr genes, among others, could effectively control the spread of MGCB from the poultry sector in LMICs.

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Section: Eastern Asia Chinamentioning

confidence: 99%

Section: Eastern Asia Chinamentioning

confidence: 99%

Section: Eastern Asia Chinamentioning

confidence: 99%

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Mobile Colistin Resistance (mcr) Gene-Containing Organisms in Poultry Sector in Low- and Middle-Income Countries: Epidemiology, Characteristics, and One Health Control Strategies

et al. 2023

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A Potential Nontraditional Approach To Combat tmexCD1-toprJ1- Mediated Tigecycline Resistance: Melatonin as a Synergistic Adjuvant of Tigecycline

Wang,

Liu,

et al. 2023

Antimicrob Agents Chemother

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The emergence of TMexCD1-TOprJ1, a novel transferable resistance-nodulation-division (RND)-type efflux pump conferring resistance to tigecycline, is now a serious public health issue in the world. Here, we found that melatonin synergistically enhanced the antibacterial efficacy of tigecycline against tmexCD1-toprJ1 -positive Klebsiella pneumoniae by disrupting the proton driving force and efflux function to promote the accumulation of tigecycline into cells, damaging cell membrane integrity and causing the leakage of cell contents.

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Coexistence of plasmid-mediated tmexCD2-toprJ2 , bla _IMP-4 , and bla _NDM-1 in Klebsiella quasipneumoniae

Ma,

Qian,

Yao

et al. 2024

Microbiol Spectr

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Klebsiella quasipneumoniae is a potential pathogen that has not been studied comprehensively. The emergence of multidrug-resistant (MDR) K. quasipneumoniae , specifically strains resistant to tigecycline and carbapenem, presents a significant challenge to clinical treatment. This investigation aimed to characterize MDR K. quasipneumoniae strain FK8966, co-carrying tmexCD2-toprJ2 , bla IMP-4 , and bla NDM-1 by plasmids. It was observed that FK8966’s MDR was primarily because of the IncHI1B-like plasmid co-carrying tmexCD2-toprJ2 and bla IMP-4 , and an IncFIB(K)/IncFII(K) plasmid harboring bla NDM-1 . Furthermore, the phylogenetic analysis revealed that IncHI1B-like plasmids carrying tmexCD2-toprJ2 were disseminated among different bacteria, specifically in China. Additionally, according to the comparative genomic analysis, the MDR regions indicated that the tmexCD2-toprJ2 gene cluster was inserted into the umuC gene, while bla IMP-4 was present in transposon Tn As3 linked to the class 1 integron ( IntI1 ). It was also observed that an ΔTn 3000 insertion with bla NDM-1 made a novel bla NDM-1 harboring IncFIB(K)/IncFII(K) plasmid. The antimicrobial resistance prevalence and phylogenetic analyses of K. quasipneumoniae strains indicated that FK8966 is a distinct MDR branch of K. quasipneumoniae . Furthermore, CRISPR-Cas system analysis showed that many K. quasipneumoniae CRISPR-Cas systems lacked spacers matching the two aforementioned novel resistance plasmids, suggesting that these resistance plasmids have the potential to disseminate within K. quasipneumoniae . Therefore, the spread of MDR K. quasipneumoniae and plasmids warrants further attention. IMPORTANCE The emergence of multidrug-resistant K. quasipneumoniae poses a great threat to clinical care, and the situation is exacerbated by the dissemination of tigecycline- and carbapenem-resistant genes. Therefore, monitoring these pathogens and their resistance plasmids is urgent and crucial. This study identified tigecycline- and carbapenem-resistant K. quasipneumoniae strain, FK8966. Furthermore, it is the first study to report the coexistence of tmexCD2-toprJ2 , bla IMP-4 , and bla NDM-1 in K. quasipneumoniae . Moreover, the CRISPR-Cas system of many K. quasipneumoniae lacks spacers that match the plasmids carried by FK8966, which are crucial for mediating resistance against tigecycline and carbapenems, indicating their potential to disseminate within K. quasipneumoniae .

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Small clone dissemination of tmexCD1-toprJ1–carrying Klebsiella pneumoniae isolates in a chicken farm

Cited by 6 publications

References 33 publications

Mobile Colistin Resistance (mcr) Gene-Containing Organisms in Poultry Sector in Low- and Middle-Income Countries: Epidemiology, Characteristics, and One Health Control Strategies

Mobile Colistin Resistance (mcr) Gene-Containing Organisms in Poultry Sector in Low- and Middle-Income Countries: Epidemiology, Characteristics, and One Health Control Strategies

A Potential Nontraditional Approach To Combat tmexCD1-toprJ1- Mediated Tigecycline Resistance: Melatonin as a Synergistic Adjuvant of Tigecycline

Coexistence of plasmid-mediated tmexCD2-toprJ2 , bla _IMP-4 , and bla _NDM-1 in Klebsiella quasipneumoniae

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Small clone dissemination of tmexCD1-toprJ1–carrying Klebsiella pneumoniae isolates in a chicken farm

Cited by 6 publications

References 33 publications

Mobile Colistin Resistance (mcr) Gene-Containing Organisms in Poultry Sector in Low- and Middle-Income Countries: Epidemiology, Characteristics, and One Health Control Strategies

Mobile Colistin Resistance (mcr) Gene-Containing Organisms in Poultry Sector in Low- and Middle-Income Countries: Epidemiology, Characteristics, and One Health Control Strategies

A Potential Nontraditional Approach To Combat tmexCD1-toprJ1- Mediated Tigecycline Resistance: Melatonin as a Synergistic Adjuvant of Tigecycline

Coexistence of plasmid-mediated tmexCD2-toprJ2 , bla IMP-4 , and bla NDM-1 in Klebsiella quasipneumoniae

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Coexistence of plasmid-mediated tmexCD2-toprJ2 , bla _IMP-4 , and bla _NDM-1 in Klebsiella quasipneumoniae