Smad3–ATF3 signaling mediates TGF-β suppression of genes encoding Phase II detoxifying proteins

Activating Transcription Factor 3 Is a Novel Repressor of the Nuclear Factor Erythroid-Derived 2–Related Factor 2 (Nrf2)–Regulated Stress Pathway

“…Forty-eight hours after transfection, cells were exposed to either vehicle control or 100 Amol/L DEM for 3 h at 37jC. ROS-mediated C-400 fluorescence was then measured (10). A, DNA precipitation (ppt ) experiments illustrating the association of ATF3 with representative AREs.…”

“…Protein extracts from NMuMG cells were analyzed as described in ref. 10. Detection was performed using Enhanced Chemiluminescence kit (Amersham Biosciences).…”

Safety and efficacy of coenzyme Q10 supplementation in early chronic Peyronie's disease: a double-blind, placebo-controlled randomized study

“…34,35 TGF-b1 inhibits antioxidant enzyme expression through Smad3/ATFdependent Nrf2 inactivation. 36 CoQ 10 decreases asmooth muscle actin and TGF-b1 expressions in Nrf2 wild-type mouse embryonic fibroblast cells. 16 Also, it has been reported that Nrf2 could initiate signal transduction pathways that regulate cell proliferation during development and in response to injury.…”

La voie Nrf2 en pathologie respiratoire

“…De manière intéressante, Nrf2 est une cible d'action du KGF (keratinocyte growth factor), facteur de croissance pour les cellules épithéliales alvéolaires aux propriétés antifibrosantes [23]. À l'inverse, le TGF-β (transforming growth factor), facteur profibrosant, supprime l'expression de Nrf2 [24]. L'augmentation de l'expression de Nrf2 et Keap1 dans l'épithélium alvéolaire hyperplasique de sujets pré-sentant une fibrose pulmonaire idiopathique a été rapportée récem- …”