Smad-3 and Smad-7 expression following anti-transforming growth factor beta 1 (TGFβ1)-treatment in irradiated rat tissue

Schultze‐Mosgau, Stefan; Blaese, Marcel A.; Grabenbauer, Gerhard G.; Wehrhan, Falk; Kopp, Jürgen; Amann, Kerstin; Rodemann, H. Peter; Rödel, Franz

doi:10.1016/j.radonc.2004.01.010

Cited by 40 publications

(24 citation statements)

References 37 publications

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“…Also, collagen deposition might prevent the contraction of muscle fibers. All these pathological elements seriously undermine the functional recovery of skeletal muscle, which is consistent with previous findings [1,[26][27][28][29] .…”

Section: Disscussionsupporting

confidence: 92%

Role of transforming growth factor-β1 in the process of fibrosis of denervated skeletal muscle

Fanbin

Chen

Liu

et al. 2011

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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In order to investigate the biological function of transforming growth factor-β1 (TGF-β1) during fibrosis in denervated skeletal muscle, we recruited sciatic nerve injury model of SD rats in which denervated gastrocnemius was isolated for analysis. At different time points after operation, denervated muscle was examined by several methods. Masson trichrome staining showed morphological changes of denervated skeletal muscle. Quantitative RT-PCR detected the rapid increase of TGF-β1 expression at mRNA level after nerve injury. It was found that a peak of TGF-β1 mRNA expression appeared one week post-operation. The expression of collagen I (COL I) mRNA was up-regulated in the nerve injury model as well, and reached highest level two weeks post-injury. Immunoblot revealed similar expression pattern of TGF-β1 and COL I in denervated muscles at protein level. In addition, we found that the area of the gastrocnemius muscle fiber was decreased gradually along with increased interstitital fibrosis. Interestingly, this pathological change could be prevented, at least partly, by local injection of TGF-β1 antibodies, which could be contributed to the reduced production of COL I by inhibiting function of TGF-β1. Taken together, in this study, we demonstrated that the expression of TGF-β1 was increased significantly in denervated skeletal muscle, which might play a crucial role during muscle fibrosis after nerve transection.

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Section: Disscussionsupporting

confidence: 92%

Role of transforming growth factor-β1 in the process of fibrosis of denervated skeletal muscle

Fanbin

Chen

Liu

et al. 2011

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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“…Following phosphorylation, binding sites for SARA (smad anchor for receptor activation), an adaptor protein, are exposed 13 . SARA mediates phosphorylation of smad2 and 3 36 , which form complexes with smad4 upon phosphorylation and translocate into the nucleus where they promote or repress particular target genes, among them a-SMA 17 . This TGFb1 signaling is necessary but not sufficient to induce the transition of the silent fibroblast to the active myofibroblast 14 .…”

Section: Discussionmentioning

confidence: 99%

Characterization of interfacial reactions between connective tissue and allogenous implants used for subdermal soft tissue augmentation

Mueller

Lee

Schultze‐Mosgau

2009

International Journal of Oral and Maxillofacial Surgery

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“…Irradiation may damage cellular elements participating in wound healing and may cause alterations in inflammatory and proliferative healing processes and changes in the expression profiles of mediators and cell matrix interactions. Finally, the changes induced by radiotherapy lead to endothelial dysfunction, pronounced fibrosis, and a decreased vascularization pattern [20].…”

Section: Discussionmentioning

confidence: 99%

Computed tomography angiography versus digital subtraction angiography in vascular mapping for planning of microsurgical reconstruction of the mandible

et al. 2005

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The aim of this work was to compare the potential of computed tomography angiography (CTA) with that of digital subtraction angiography (DSA) in vascular mapping of the external carotid artery (ECA) branches for planning of microvascular reconstructions of the mandible with osteomyocutaneous flaps. In 15 patients CTA and DSA were performed prior to surgery. Selective common carotid angiograms were acquired in two projection for both sides of the neck. Sixteen-slice spiral computed tomography was performed with a dual-phase protocol, using the arterial phase images for 3D CTA reconstruction. Thin-slab maximum intensity projections and volume rendering were employed for postprocessing of CTA data. The detectability of the different ECA branches in CTA and DSA was evaluated by two examiners. No statistically significant differences between CTA and DSA (p=0.097) were found for identifying branches relevant for microsurgery. DSA was superior to CTA if more peripheral ECA branches were included (P=0.030). CTA proved to be a promising alternative to DSA in vascular mapping for planning of microvascular reconstruction of the mandible.

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Smad-3 and Smad-7 expression following anti-transforming growth factor beta 1 (TGFβ1)-treatment in irradiated rat tissue

Cited by 40 publications

References 37 publications

Role of transforming growth factor-β1 in the process of fibrosis of denervated skeletal muscle

Role of transforming growth factor-β1 in the process of fibrosis of denervated skeletal muscle

Characterization of interfacial reactions between connective tissue and allogenous implants used for subdermal soft tissue augmentation

Computed tomography angiography versus digital subtraction angiography in vascular mapping for planning of microsurgical reconstruction of the mandible

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