SlyA, a MarR Family Transcriptional Regulator, Is Essential for Virulence in
            <i>Dickeya dadantii</i>
            3937

Haque, MM; Kabir, Md. Shahinur; Aini, Luqman Qurata; Hirata, Haruyuki; Tsuyumu, Shinji

doi:10.1128/jb.01205-09

Cited by 17 publications

(34 citation statements)

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“…Susceptibility to both oxidative stress (H 2 O 2 ) and antimicrobial peptides (polymixin B) was determined as described by Haque et al (2009). H 2 O 2 at 6 mM or 1 mg polymixin B ml 21 was added to cells grown to stationary phase in M63 glycerol medium.…”

Section: Methodsmentioning

confidence: 99%

Inactivation of pecS restores the virulence of mutants devoid of osmoregulated periplasmic glucans in the phytopathogenic bacterium Dickeya dadantii

2014

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Dickeya dadantii is a phytopathogenic enterobacterium that causes soft rot disease in a wide range of plant species. Maceration, an apparent symptom of the disease, is the result of the synthesis and secretion of a set of plant cell wall-degrading enzymes (PCWDEs), but many additional factors are required for full virulence. Among these, osmoregulated periplasmic glucans (OPGs) and the PecS transcriptional regulator are essential virulence factors. Several cellular functions are controlled by both OPGs and PecS. Strains devoid of OPGs display a pleiotropic phenotype including total loss of virulence, loss of motility and severe reduction in the synthesis of PCWDEs. PecS is one of the major regulators of virulence in D. dadantii, acting mainly as a repressor of various cellular functions including virulence, motility and synthesis of PCWDEs. The present study shows that inactivation of the pecS gene restored virulence in a D. dadantii strain devoid of OPGs, indicating that PecS cannot be de-repressed in strains devoid of OPGs.

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Section: Methodsmentioning

confidence: 99%

Inactivation of pecS restores the virulence of mutants devoid of osmoregulated periplasmic glucans in the phytopathogenic bacterium Dickeya dadantii

2014

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“…Smu.124, which may function as a transcription regulator/repressor, belongs to the MarR family. Members of this family have been shown to be involved in regulation of virulence factors, catabolism of aromatic compounds, and response to antibiotics and oxidative stress and act to prevent gene expression by sterically hindering transcription (3,14,22). Smu.124 is located upstream of four genes (Smu.127 to Smu.130) involved in the metabolism of pyruvate, which is a product of glycolysis and amino acid metabolism.…”

Section: Figmentioning

confidence: 99%

Characterization of a Glutamate Transporter Operon, glnQHMP , in Streptococcus mutans and Its Role in Acid Tolerance

et al. 2010

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Glutamate contributes to the acid tolerance response (ATR) of many Gram-negative and Gram-positive bacteria, but its role in the ATR of the oral bacterium Streptococcus mutans is unknown. This study describes the discovery and characterization of a glutamate transporter operon designated glnQHMP (Smu.1519 to Smu.1522) and investigates its potential role in acid tolerance. Deletion of glnQHMP resulted in a 95% reduction in transport of radiolabeled glutamate compared to the wild-type UA159 strain. The addition of glutamate to metabolizing UA159 cells resulted in an increased production of acidic end products, whereas the glnQHMP mutant produced less lactic acid than UA159, suggesting a link between glutamate metabolism and acid production and possible acid tolerance. To investigate this possibility, we conducted a microarray analysis with glutamate and under pH 5.5 and pH 7.5 conditions which showed that expression of the glnQHMP operon was downregulated by both glutamate and mild acid. We also measured the growth kinetics of UA159 and its glnQHMP-negative derivative at pH 5.5 and found that the mutant doubled at a much slower rate than the parent strain but survived at pH 3.5 significantly better than the wild type. Taken together, these findings support the involvement of the glutamate transporter operon glnQHMP in the acid tolerance response in S. mutans.

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“…However, despite the importance of ions in virulence, relatively little is known about regulation in response to most of these signals. As with related genera, SRE use the PhoPQ two-component system and SlyA, which responds to magnesium levels, to monitor their environment and control expression of virulence genes (42,52,53,90,92) . In addition to regulation, ions also affect the efficacy of virulence proteins, and this has been most clearly demonstrated with SRE pectate lyases.…”

Section: Ahls Produced By Pectobacterium or By Other Bacteria Can Bmentioning

confidence: 99%

The Role of Secretion Systems and Small Molecules in Soft-Rot Enterobacteriaceae Pathogenicity

Charkowski¹,

Blanco

Condemine

et al. 2012

Annu. Rev. Phytopathol.

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215

251

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Soft-rot Enterobacteriaceae (SRE), which belong to the genera Pectobacterium and Dickeya, consist mainly of broad host-range pathogens that cause wilt, rot, and blackleg diseases on a wide range of plants. They are found in plants, insects, soil, and water in agricultural regions worldwide. SRE encode all six known protein secretion systems present in gram-negative bacteria, and these systems are involved in attacking host plants and competing bacteria. They also produce and detect multiple types of small molecules to coordinate pathogenesis, modify the plant environment, attack competing microbes, and perhaps to attract insect vectors. This review integrates new information about the role protein secretion and detection and production of ions and small molecules play in soft-rot pathogenicity.

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SlyA, a MarR Family Transcriptional Regulator, Is Essential for Virulence in Dickeya dadantii 3937

Cited by 17 publications

References 0 publications

Inactivation of pecS restores the virulence of mutants devoid of osmoregulated periplasmic glucans in the phytopathogenic bacterium Dickeya dadantii

Inactivation of pecS restores the virulence of mutants devoid of osmoregulated periplasmic glucans in the phytopathogenic bacterium Dickeya dadantii

Characterization of a Glutamate Transporter Operon, glnQHMP , in Streptococcus mutans and Its Role in Acid Tolerance

The Role of Secretion Systems and Small Molecules in Soft-Rot Enterobacteriaceae Pathogenicity

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