Slower Rates of Clearance of Viral Load and Virus‐Containing Immune Complexes in Patients with Dengue Hemorrhagic Fever

Wang, Wei‐Kung; Chen, Huiling; Yang, Chia‐Ling; Hsieh, Szu-Chia; Juan, Chung-Chou; Chang, Shyang-Jye; Yu, Cheng‐Ching; Lin, Lihui; Huang, Jiansheng; King, Chwan-Chuen

doi:10.1086/507635

Cited by 157 publications

(166 citation statements)

References 41 publications

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“…Studies that use PCR as an indicator for dengue virus detection show that viremia is higher in DHF versus DF patients. [30][31][32][33] Although antibody-virus complexes and neutralized virus would impact viral biological assays such as viral isolation, they would not likely impact PCR readouts.…”

Section: Discussionmentioning

confidence: 99%

Factors Influencing Dengue Virus Isolation by C6/36 Cell Culture and Mosquito Inoculation of Nested PCR-Positive Clinical Samples

Jarman

Nisalak

Anderson

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Abstract. Dengue viral isolation is necessary for definitive diagnosis, pathogenesis and evolutionary research, vaccine candidates, and diagnostic materials. Using standardized techniques, we analyzed isolation rates of 1,544 randomly selected polymerase chain reaction (PCR)-positive samples, representing all four dengue serotypes, from patients with serologically confirmed dengue infections and evaluated whether clinical and laboratory results could be predictive of isolation using standard and mosquito isolation techniques. Viruses were isolated from 62.5% of the samples by direct application to C6/36 cells and increased to 79.4% when amplifying C6/36 negative samples by intrathorasic inoculation in Toxyrhynchites splendens mosquitoes. High viremia, measured by reverse transcriptase (RT)-PCR, was a strong predictor for viral isolation by either method. Isolation was most successful in samples collected early in the disease, had low antibody levels, temperatures greater than 38°C, and had a final clinical diagnosis of dengue fever. Dengue serotypes also played a role in the success of viral isolation.

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Section: Discussionmentioning

confidence: 99%

Factors Influencing Dengue Virus Isolation by C6/36 Cell Culture and Mosquito Inoculation of Nested PCR-Positive Clinical Samples

Jarman

Nisalak

Anderson

et al. 2011

The American Society of Tropical Medicine and Hygiene

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“…12,13 Flaviviral infections, such as yellow fever, West Nile fever, and especially dengue fever, are among the most common arboviral infections worldwide, but the resulting symptoms are non-specific, leading to misdiagnosis. [14][15][16] For example, chikungunya fever, caused by the alphavirus chikungunya virus, is an emerging infectious disease in endemic and non-endemic regions and can be misdiagnosed as dengue fever, which is largely sympatric in the Old World. [16][17][18][19][20][21] Because the clinical presentation of arboviral disease is often non-specific, reliance on diagnostic testing becomes necessary for accurate differentiation.…”

mentioning

confidence: 99%

Serologic Evidence of Arboviral Infections among Humans in Kenya

Sutherland

Cash

Huang

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Abstract. Outbreaks of arthropod-borne viral infections occur periodically across Kenya. However, limited surveillance takes place during interepidemic periods. Using serum samples obtained from asymptomatic persons across Kenya in 2000-2004, we assessed (by indirect immunofluorescent assay) prevalence of IgG against yellow fever virus (YFV), West Nile virus (WNV), tick-borne encephalitis virus (TBEV), dengue virus serotypes 1-4 (DENV1-4), and chikungunya virus (CHIKV). Older persons on the Indian Ocean coast were more likely to be seropositive than children inland: YFV = 42% versus 6%, WNV = 29% versus 6%, TBEV = 16% versus 6%, DENV-1 = 63% versus 9%, DENV-2 = 67% versus 7%, DENV-3 = 55% versus 6%, DENV-4 = 44% versus 8%, and CHIKV = 37% versus 20%. Among inland samples, children in lowlands were more likely to be seropositive for CHIKV (42% versus 0%) than children in highlands. In Kenya, transmission of arboviral infection continues between known epidemics and remains common across the country.

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“…In addition, significant cross reactivity between DENV serotypes was shown in CD8(+) T-cells after a naturally acquired primary infection with differential T-cell receptor signalling after stimulation with homologous and heterologous peptides (Friberg et al 2011). DENV entry is facilitated by the phagocytosis of immune complexes via Fc receptors on monocytes (Daughaday et al 1981) and studies of immune responses have reported circulating immune complexes and complement activation during the fever-to-defervescence period in which haemorrhage, plasma leakage and/or circulatory failure occur in patients with DHF and/or OSS (Ruangjirachuporn et al 1979, Wang et al 2006.…”

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confidence: 99%

Environmental influences on antibody-enhanced dengue disease outcomes

Diniz

Fôro

Turiel³

et al. 2012

Mem. Inst. Oswaldo Cruz

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Because an enriched environment (EE) enhances T-cell activity and T-lymphocytes contribute to immunopathogenesis during heterologous dengue virus (DENV) infections, we hypothesised that an EE increases dengue severity. To compare single serotype (SS) and antibody-enhanced disease (AED) infections regimens, serial intraperitoneal were performed with DENV3 (genotype III) infected brain homogenate or anti-DENV2 hyperimmune serum followed 24 h later by DENV3 (genotype III) infected brain homogenate. Compared AED for which significant differences were detected between the EE and impoverished environmental (IE) groups (Kaplan-Meyer log-rank test, p = 0.0025), no significant differences were detected between the SS experimental groups (Kaplan-Meyer log-rank test, p = 0.089). Survival curves from EE and IE animals infected with the AED regimen were extended after corticoid injection and this effect was greater in the EE than in the IE group (Kaplan-Meyer log-rank test, p = 0.0162). Under the AED regimen the EE group showed more intense clinical signs than the IE group. Dyspnoea, tremor, hunched posture, ruffled fur, immobility, pre-terminal paralysis, shock and death were associated with dominant T-lymphocytic hyperplasia and presence of viral antigens in the liver and lungs. We propose that the increased expansion of these memory T-cells and serotype cross-reactive antibodies facilitates the infection of these cells by DENV and that these events correlate with disease severity in an EE

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Slower Rates of Clearance of Viral Load and Virus‐Containing Immune Complexes in Patients with Dengue Hemorrhagic Fever

Cited by 157 publications

References 41 publications

Factors Influencing Dengue Virus Isolation by C6/36 Cell Culture and Mosquito Inoculation of Nested PCR-Positive Clinical Samples

Factors Influencing Dengue Virus Isolation by C6/36 Cell Culture and Mosquito Inoculation of Nested PCR-Positive Clinical Samples

Serologic Evidence of Arboviral Infections among Humans in Kenya

Environmental influences on antibody-enhanced dengue disease outcomes

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