Slow‐release nanoparticle‐encapsulated delivery system for laryngeal injection

Kolachala, Vasantha L.; Henriquez, Oswaldo A.; Shams, Samantha; Golub, Justin S.; Kim, Young Tae; Laroui, Hamed; Torres-González, Edilson; Brigham, Kenneth L.; Rojas, Mauricio; Bellamkonda, Ravi V.; Johns, Michael M.

doi:10.1002/lary.20856

Cited by 17 publications

(12 citation statements)

References 20 publications

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“…Direct effects of nanotechnology on operative surgery are, at this time, principally concerned with materials that can be implanted. These can be particulate so as to be injectable into tissues, for example, to maintain sustained drug levels in a particular tissue bed 27. Nanoparticles have also been proposed to enhance and control the freezing efficiency of cryo‐surgery 28.…”

Section: Nanotechnology In Surgerymentioning

confidence: 99%

Removal of Natural Free Estrogens and their Conjugates in a Municipal Wastewater Treatment Plant

Liu

Kanjo

Mizutani

2011

CLEAN Soil Air Water

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Removal of natural free estrogens and estrogen conjugates in a municipal wastewater treatment plant (WWTP) was investigated and analyzed by GC-MS, in which estrogen conjugates were first transformed to their corresponding free estrogens with an acid solvolysis procedure before their analysis. Natural free estrogens, E1-3-sulfate (E1-3S), and E3-3-sulfate (E3-3S) were detected with high concentrations in both the influent and effluent of the primary settling tank (PS), while no estrogen glucuronides were detected in any of the monitored wastewater samples. Regarding their removal efficiencies, all were almost completely removed, except for E1 with only a minor decrease. The estrogenic/androgenic removal of the same WWTP was also evaluated with estrogen receptor (androgen receptor) (ER (AR))-binding assays, in which the removal efficiencies for E2 equivalents (EEQ) or testosterone equivalents (TEQ) were 68.5 and 72.2%. In addition, the chemically calculated EEQ from natural estrogens were about 20.6-39.3% that of the ER-binding assay, in which E3 contributed the biggest proportion in both the influent and PS, while the calculated value of E1 increased from only 6.7% in the influent to as high as 20.6% in the effluent. IntroductionRegarding possible adverse effects on humans and wildlife, endocrine disrupting compounds (EDCs) have been extensively monitored and detected in different environmental samples [1][2][3][4][5]. In municipal wastewater treatment plants (WWTPs), natural estrogens are regarded as one of the most important sources, which have been widely studied [6][7][8]. Although estrogens in human urine were mainly in their sulfate/glucuronide conjugates [9], they were believed to have been bio-transformed to their free estrogens by fecal bacteria before they reach WWTPs [10]. However, much recent studies have shown that estrogen conjugates existed not only in the influent of WWTPs, but also in the effluent [11][12][13]. As estrogen conjugates in effluents with low estrogenic potencies will be further bio-transformed to their free estrogens after their entry into the receiving bodies. These conjugates can be expected to act with the same function as their corresponding free estrogens in the effluent, which may have adverse effects on the aquatic organisms. Therefore, removal performance for both free estrogens and their conjugates in wastewater by WWTPs should be evaluated.Although LC-MS/MS has been applied to environmental samples for simultaneous analysis of free estrogens and their conjugates [11][12][13], it is not as widely used as GC-MS due to its higher price. Moreover, as estrogen conjugates are highly polar compounds, purification procedures to handle them in wastewater samples were complex or the recovery efficiencies were very poor [13][14][15][16]. Analytical methods for estrogen conjugates by LC-MS/MS without purification procedures were also available with a low detection limit, but on these occasions ion suppression phenomena are likely to occur [17,18]. In a previous work, simultaneous anal...

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Section: Nanotechnology In Surgerymentioning

confidence: 99%

Removal of Natural Free Estrogens and their Conjugates in a Municipal Wastewater Treatment Plant

Liu

Kanjo

Mizutani

2011

CLEAN Soil Air Water

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“…Technologies such as slowreleasing nanoparticle delivery systems and cell-responsive hydrogels could regulate the evolution of gradients in a more natural, temporal manner. [147] The efficacy of FGMs as model systems is dependent upon ease of fabrication, simplicity of use, and reproducibility in resulting data. For these FGMs to be widely available, the methods employed must be simple and should be high throughput in nature to ensure translation to the clinic.…”

Section: Conclusion and Future Outlookmentioning

confidence: 99%

Functionally Graded Biomaterials for Use as Model Systems and Replacement Tissues

Lowen

Leach

2020

Adv Funct Materials

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The heterogeneity of native tissues requires complex materials to provide suitable substitutes for model systems and replacement tissues. Functionally graded materials have the potential to address this challenge by mimicking the gradients in heterogeneous tissues such as porosity, mineralization, and fiber alignment to influence strength, ductility, and cell signaling. Advancements in microfluidics, electrospinning, and 3D printing enable the creation of increasingly complex gradient materials that further the understanding of physiological gradients. The combination of these methods enables rapid prototyping of constructs with high spatial resolution. However, successful translation of these gradients requires both spatial and temporal presentation of cues to model the complexity of native tissues that few materials have demonstrated. This Review highlights recent strategies to engineer functionally graded materials for the modeling and repair of heterogeneous tissues, together with a description of how cells interact with various gradients.

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“…As an alternative approach, exogenous delivery of biomolecules can be used for treating a variety of laryngeal conditions [323]. For example, steroid injections have been used for the reduction of vocal fold scarring post-surgery [324].…”

Section: Progress Towards Engineering/regenerating Tissue-type-specifmentioning

confidence: 99%

“…Hence, in order to improve the bioavailability and prolong the efficacy of the therapeutic agents, while avoiding repeated injections, they can be encapsulated in nanoparticles for sustained release. Kolachala et al have demonstrated the efficacy of laryngeal therapy involving HGF delivered from PLGA nanoparticles over repeated injections [323] in decreasing procollagen mRNA, the production of which is undesirable in vocal fold regeneration. This illustrates the utility of nanoparticles for controlled delivery of therapies for vocal fold regeneration.…”

Section: Progress Towards Engineering/regenerating Tissue-type-specifmentioning

confidence: 99%

Advances in biomimetic regeneration of elastic matrix structures

Balakrishnan

Bashur

Ramamurthi

2012

Drug Deliv. and Transl. Res.

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Elastin is a vital component of the extracellular matrix, providing soft connective tissues with the property of elastic recoil following deformation and regulating the cellular response via biomechanical transduction to maintain tissue homeostasis. The limited ability of most adult cells to synthesize elastin precursors and assemble them into mature crosslinked structures has hindered the development of functional tissue-engineered constructs that exhibit the structure and biomechanics of normal native elastic tissues in the body. In diseased tissues, the chronic overexpression of proteolytic enzymes can cause significant matrix degradation, to further limit the accumulation and quality (e.g., fiber formation) of newly deposited elastic matrix. This review provides an overview of the role and importance of elastin and elastic matrix in soft tissues, the challenges to elastic matrix generation in vitro and to regenerative elastic matrix repair in vivo, current biomolecular strategies to enhance elastin deposition and matrix assembly, and the need to concurrently inhibit proteolytic matrix disruption for improving the quantity and quality of elastogenesis. The review further presents biomaterial-based options using scaffolds and nanocarriers for spatio-temporal control over the presentation and release of these biomolecules, to enable biomimetic assembly of clinically relevant native elastic matrix-like superstructures. Finally, this review provides an overview of recent advances and prospects for the application of these strategies to regenerating tissue-type specific elastic matrix structures and superstructures.

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Slow‐release nanoparticle‐encapsulated delivery system for laryngeal injection

Abstract: The results demonstrate the potential utility of nanoparticle encapsulation as an effective method for long-term delivery of specific drugs and biologically active substances to the larynx.

Cited by 17 publications

References 20 publications

Removal of Natural Free Estrogens and their Conjugates in a Municipal Wastewater Treatment Plant

Removal of Natural Free Estrogens and their Conjugates in a Municipal Wastewater Treatment Plant

Functionally Graded Biomaterials for Use as Model Systems and Replacement Tissues

Advances in biomimetic regeneration of elastic matrix structures

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