Slow Growth in Vitro Culture for Conservation of Hancornia Speciosa Gomes

Abstract

Hancornia speciosa Gomes is a fruit species endemic to the Cerrado and coastal plains of Northeast of Brazil, with great economic, nutritional, ecological, and medicinal potential. This study aimed to evaluate the effect of sorbitol and sucrose as osmotic regulators on the in vitro growth of mangabeira, aiming at conservation by slow growth. The explants were obtained from in vitro germinated seedlings and inoculated in MS medium supplemented with sucrose (15 and 30 g L-1) and sorbitol (0, 5, 10 and 15 g L-1). The experimental design was completely randomized with 20 repetitions in a 4 x 2 factorial arrangement (sorbitol x sucrose concentrations). The evaluations were performed at 30, 60 90 and 120 days of incubation. The analyzed variables were number of nodes/budding, number of leaves, leaf abscission, leaf color and survival of explants. The data were statistically analyzed by generalized linear model analysis. The results indicated a significant difference between the osmotic regulators and the culture time for all variables. Sorbitol showed a more pronounced growth-reducing effect than sucrose. The use of 30 g L-1 sucrose combined with 10 or 20 g L-1 sorbitol reduced the growth in a critical way, making it clear that the water stress caused was not tolerated by the plants, negatively interfering in its development. Treatment with 15 g L-1 sucrose combined with 5 g L-1 sorbitol promoted the best result, allowing the conservation of plants for 120 days.

Advances and perspectives in the propagation of mangabeira (Hancornia speciosa Gomes) - review on a tropical fruit tree with socioeconomic importance

“…• The best results for plant conservation are obtained by adding 30 g L -1 of sucrose to the cultivation medium and carrying out in vitro conservation for up to 90 days. (Santos et al, 2011;Santos et al, 2016;Pires et al, 2020;Oliveira & Aloufa, 2022;Pires et al, 2022) Cryopreservation…”

Cinchona officinalis L. in vitro conservation by means of slow-growth storage and cryopreservation techniques

“…In other species, in vitro assessment of slow growth showed that explants had storage cycles ranging from 45 days (Hunter, 1986) to 28 months without subcultures (Gianní and Sottile, 2015;Oliveira and Ibrahim, 2022;Panis et al, 2020).…”

“…The conservation of tissues through in vitro cultivation techniques has solidified itself as a valuable alternative, deploying two fundamental approaches: slow-growth storage (achieved by adjusting conditions that directly influence the development of explants) and reducing the frequency of required subcultures (Cruz et al 2013;. This method allows extending the duration of a subculture cycle from a few 3 weeks to 12 months or more (Gianní and Sottile 2015;Oliveira and Ibrahim 2022;Panis et al 2020;Radhamani and Sivaraj 2013;Li et al 2014). On the other hand, tissue cryopreservation involves the suspension of growth for indefinite periods (Paunescu 2009;Ai et al 2012;Reed et al 2011;Sen-Rong and Ming-Hua 2012;Cruz et al 2013;Panis et al 2020;Popova et al 2023).…”

In Vitro Conservation through Slow Growth Storage Technique of Fruit Species: An Overview of the Last 10 Years

“…Recently, de Oliviera and Aloufa [ 66 ] tested the effect of osmotic compounds added to the culture medium to slow the growth of Hancornia speciosa shoots maintained at 25 °C (30 μmol m −2 s −1 , 16 h photoperiod). Different concentrations of sucrose and sorbitol were tested for various storage times.…”

“…The combination of 15 g L −1 sucrose with 5 g L −1 sorbitol gave the best result (95% survival), allowing the conservation of the shoots for 4 months. In contrast, higher concentrations of sucrose (30 g L −1 ) and sorbitol (10 or 20 g L −1 ) showed toxic and stressful effects on shoot survival with thin stems, reduced or absent leaves, high oxidation incidence and greater callus formation at the base of the explants [ 66 ].…”