Slow-Flow Ultrasound Localization Microscopy Using Recondensation of Perfluoropentane Nanodroplets

Burgess, Mark; Aliabouzar, Mitra; Aguilar, Christian; Fabiilli, Mario L.; Ketterling, Jeffrey A.

doi:10.1016/j.ultrasmedbio.2021.12.007

Cited by 12 publications

(8 citation statements)

References 66 publications

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“…The breaks between imaging sequences may affect correction of large motion e.g., for cardiac SR imaging. Some changes to the imaging sequence might be required to minimize motion artefacts such as higher frame rates, wider transmit beams, fewer focused transmissions, or a longer period of continuous flow imaging [55]. Droplet vaporization and imaging may be asynchronous.…”

Section: Discussionmentioning

confidence: 99%

Fast and Selective Super-Resolution Ultrasound In Vivo With Acoustically Activated Nanodroplets

Riemer

Toulemonde

Yan

et al. 2023

IEEE Trans. Med. Imaging

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Perfusion by the microcirculation is key to the development, maintenance and pathology of tissue. Its measurement with high spatiotemporal resolution is consequently valuable but remains a challenge in deep tissue. Ultrasound Localization Microscopy (ULM) provides very high spatiotemporal resolution but the use of microbubbles requires low contrast agent concentrations, a long acquisition time, and gives little control over the spatial and temporal distribution of the microbubbles. The present study is the first to demonstrate Acoustic Wave Sparsely-Activated Localization Microscopy (AWSALM) and fast-AWSALM for in vivo super-resolution ultrasound imaging, offering contrast on demand and vascular selectivity. Three different formulations of acoustically activatable contrast agents were used. We demonstrate their use with ultrasound mechanical indices well within recommended safety limits to enable fast on-demand sparse activation and destruction at very high agent concentrations. We produce super-localization maps of the rabbit renal vasculature with acquisition times between 5.5 s and 0.25 s, and a 4-fold improvement in spatial resolution. We present the unique selectivity of AWSALM in visualizing specific vascular branches and downstream microvasculature, and we show super-localized kidney structures in systole (0.25 s) and diastole (0.25 s) with fast-AWSALM outdoing microbubble based ULM. In conclusion, we demonstrate the feasibility of fast and selective measurement of microvascular dynamics in vivo with subwavelength resolution using ultrasound and acoustically activatable nanodroplet contrast agents.

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Section: Discussionmentioning

confidence: 99%

Fast and Selective Super-Resolution Ultrasound In Vivo With Acoustically Activated Nanodroplets

Riemer

Toulemonde

Yan

et al. 2023

IEEE Trans. Med. Imaging

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“…The findings here can be useful in applications beyond drug delivery. For example, repeated vaporization and recondensation of phase-shift emulsions can be leveraged for an on-and-off blinking contrast signal between consecutive ultrasound pulses, offering better localization of bubble signal from tissue [39] . The ADV-induced stable or transient bubble formation can also be utilized to tune micromechanical properties of strain stiffening biomaterials in an on-demand, non-invasive manner [22] , [24] .…”

Section: Discussionmentioning

confidence: 99%

Multi-time scale characterization of acoustic droplet vaporization and payload release of phase-shift emulsions using high-speed microscopy

Aliabouzar

Kripfgans

Estrada

et al. 2022

Ultrasonics Sonochemistry

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“…Once these filtered contrast signals were obtained, these spatially isolated signals were localized. As the point spread function of the imaging system can define the acoustic response to a single isolated point scatterer, the coordinates of each signal can be identified, and its accuracy is significantly higher than that of diffraction-limited resolution . After localization processing, these localized signals between the consecutive frames can be tracked to define the paths and velocity of microbubbles within the microvasculature.…”

Section: Principles Of Ultrasound Localization Microscopymentioning

confidence: 99%

“…Such a requirement for microbubble relocation or replenishment significantly increases ULM acquisition time, especially for microvasculature with relatively slow flow rates, such as capillary networks, while nanodroplets are independent of flow rate. 14,15 To overcome this challenge, previous studies have shown that a relatively high concentration of low-boiling-point nanodroplets can be randomly and sparsely activated within the clinical safety limits and then deactivated as required by controlling the applied ultrasound amplitude, thus demonstrating the ultrasonic counterpart of PALM at depth. 16,17 Nanodroplets are converted from liquid to microbubbles under acoustic or laser activation, and the resulting spatially stationary, temporally transient microbubbles will realize the formation of SR-ULM before recondensation into a liquid nanodroplet state.…”

Section: ■ Highlightsmentioning

confidence: 99%

“…Flow is crucial here because sparse activation of microbubbles is not possible, unlike the case for OLM. Such a requirement for microbubble relocation or replenishment significantly increases ULM acquisition time, especially for microvasculature with relatively slow flow rates, such as capillary networks, while nanodroplets are independent of flow rate. , …”

Section: Highlightsmentioning

confidence: 99%

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Nanodroplet-Based Super-Resolution Ultrasound Localization Microscopy

Zhang,

Liao,

et al. 2023

ACS Sens.

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Over the past decade, super-resolution ultrasound localization microscopy (SR-ULM) has revolutionized ultrasound imaging with its capability to resolve the microvascular structures below the ultrasound diffraction limit. The introduction of this imaging technique enables the visualization, quantification, and characterization of tissue microvasculature. The early implementations of SR-ULM utilize microbubbles (MBs) that require a long image acquisition time due to the requirement of capturing sparsely isolated microbubble signals. The next-generation SR-ULM employs nanodroplets that have the potential to significantly reduce the image acquisition time without sacrificing the resolution. This review discusses various nanodroplet-based ultrasound localization microscopy techniques and their corresponding imaging mechanisms. A summary is given on the preclinical applications of SR-ULM with nanodroplets, and the challenges in the clinical translation of nanodroplet-based SR-ULM are presented while discussing the future perspectives. In conclusion, ultrasound localization microscopy is a promising microvasculature imaging technology that can provide new diagnostic and prognostic information for a wide range of pathologies, such as cancer, heart conditions, and autoimmune diseases, and enable personalized treatment monitoring at a microlevel.

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Slow-Flow Ultrasound Localization Microscopy Using Recondensation of Perfluoropentane Nanodroplets

Cited by 12 publications

References 66 publications

Fast and Selective Super-Resolution Ultrasound In Vivo With Acoustically Activated Nanodroplets

Fast and Selective Super-Resolution Ultrasound In Vivo With Acoustically Activated Nanodroplets

Multi-time scale characterization of acoustic droplet vaporization and payload release of phase-shift emulsions using high-speed microscopy

Nanodroplet-Based Super-Resolution Ultrasound Localization Microscopy

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