SLL1, which encodes a member of the stearoyl-acyl carrier protein fatty acid desaturase family, is involved in cell elongation in lateral roots via regulation of fatty acid content in rice

Shelley, Israt J.; Nishiuchi, Shunsaku; Shibata, K; Inukai, Yoshiaki

doi:10.1016/j.plantsci.2013.01.003

Cited by 17 publications

(12 citation statements)

References 30 publications

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“…In the badc1 badc2 double mutant (which showed a root growth phenotype), the fatty acid content per seed weight was reduced by ;25% from the wild type ( Fig. 9; Supplemental Data S4), but as noted earlier, this effect maybe a secondary consequence of the reduced root growth (Shelley et al, 2013;Ding et al, 2015;Liu et al, 2019). The effect of the other viable double mutant (i.e.…”

Section: Biochemical Characterization Of Badc Mutantsmentioning

confidence: 52%

“…8; Supplmental Data S2 and S3) these latter multiple effects may be a secondary consequence of reduced root growth, which was the first detectable phenotypic effect that developed in plants expressing only BADC3. Indeed, such complex phenotypes occur in a number of other fatty acid metabolism mutants that primarily first affect root growth (Shelley et al, 2013;Ding et al, 2015). Collectively these characterizations led to the conclusion that BADC2 and BADC3 share the highest functional redundancy, but that redundancy is not symmetrical, with BADC2 being able to replace almost all BADC3 functions, but not vice versa.…”

Section: Subcomplexmentioning

confidence: 99%

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Non-Catalytic Subunits Facilitate Quaternary Organization of Plastidic Acetyl-CoA Carboxylase

et al. 2019

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Arabidopsis (Arabidopsis thaliana), like most dicotyledonous plants, expresses a multicomponent, heteromeric acetyl-CoA carboxylase (htACCase), which catalyzes the generation of the malonyl-CoA precursor of de novo fatty acid biosynthesis. This enzyme consists of four catalytic subunits: biotin carboxylase (BC), carboxyltransferase (CT)-a, CT-b, and biotin carboxyl carrier protein (BCCP1 or BCCP2). By coexpressing combinations of components in a bacterial expression system, we demonstrate noncatalytic BADCs facilitate the assembly and activation of BCCP-BADC-BC subcomplexes catalyzing the bicarbonate-dependent hydrolysis of ATP, which is the first half-reaction catalyzed by the htACCase enzyme. Although BADC proteins do not directly impact formation of the CT-ab subcomplex, the BADC-facilitated BCCP-BADC-BC subcomplex can more readily interact with the CT-ab subcomplex to facilitate the generation of malonyl-CoA. The Arabidopsis genome encodes three BADC isoforms (BADC1, BADC2, and BADC3), and BADC2 and BADC3 (rather than BADC1), in combination with BCCP1, best support this quaternary-structural organization and catalytic activation of the htACCase enzyme. Physiological genetic studies validate these attributes as Arabidopsis double mutants singularly expressing BADC2, BADC3, or BADC1 present increasingly greater deleterious impacts on morphological and biochemical phenotypes. Specifically, plants expressing only BADC2 develop normally, plants only expressing BADC3 suffer a stunted root-growth phenotype, and plants expressing only BADC1 are embryo-lethal. The latter phenotype may also be associated with the distinct suborganelle localization of BADC1 in plastids as compared to the localization of the other two BADC homologs. These finding can inspire novel strategies to improve the biological sources of fats and oils for dietary and industrial applications.

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Section: Biochemical Characterization Of Badc Mutantsmentioning

confidence: 52%

Section: Subcomplexmentioning

confidence: 99%

Non-Catalytic Subunits Facilitate Quaternary Organization of Plastidic Acetyl-CoA Carboxylase

et al. 2019

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“…RNAi of the SQS plants grown in plates with Yoshida's nutrient solution with 1.2% agar showed increased root length and an enhanced number of lateral roots (Manavalan et al 2012). SLL1 encodes stearoyl-acyl carrier protein and over-expression SLL1 plants produced signi cantly longer lateral roots compared to wild-type plants (Shelley et al 2013). Meanwhile, one region containing the lead SNP seq8_15949787 belongs to a previously reported QTL (Lilley et al 1996) of root dehydration tolerance.…”

Section: Identifying the Candidate Genes Associated With The Rooting mentioning

confidence: 94%

Genome-wide association study of rice rooting ability at the seedling stage

Yang

et al. 2020

Preprint

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BackgroundRice rooting ability is a complex agronomical trait that displays heterosis and plays an important role in rice growth and production. Only a few quantitative trait loci (QTLs) have been identified by bi-parental population. More genes or QTLs are required to dissect the genetic architecture of rice rooting ability.ResultsTo characterize the genetic basis for rice rooting ability, we used a natural rice population, genotyped by a 90K single nucleotide polymorphism (SNP) array, to identify the loci associated with rooting-related traits through the genome-wide association study (GWAS). Population structure analysis divided the natural population into two subgroups: indica and japonica. We measured four traits for evaluating rice rooting ability, namely root growth ability (RGA), maximum root length (MRL), root length (RL), and root number (RN). Using the association study in three panels consisting of one for the full population, one for indica, and one for japonica, 24 SNPs associated with rooting ability-related traits were identified. Through comparison of the relative expression levels and DNA sequences between germplasm with extreme phenotypes, results showed that LOC_Os05g11810 had non-synonymous variations at the coding region, which may cause differences in root number, and that the expression levels of LOC_Os04g09900 and LOC_Os04g10060 are closely associated with root length variation.ConclusionsThrough evaluation of the rice rooting ability-related traits and the association mapping, we provided useful information for understanding the genetic basis of rice rooting ability and also identified some candidate genes and molecular markers for rice root breeding.

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“…RNAi of the SQS (farnesyltransferase/squalene synthase) plants grown in plates with Yoshida's nutrient solution with 1.2% agar show increased root length and an enhanced number of lateral roots (Manavalan et al 2012). Over-expression SLL1 plants produced signi cantly longer lateral roots compared to wild-type plants (Shelley et al 2013). Meanwhile, one region containing the lead SNP seq8_15949787 belonged to a previously reported QTL (Lilley et al 1996) of root dehydration tolerance.…”

Section: Identifying the Candidate Genes Associated With The Rooting mentioning

confidence: 99%

Genome-Wide Association Study of Rice Rooting Ability at the Seedling Stage

Yang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Rice rooting ability is a complex agronomical trait that displays heterosis and plays an important role in rice growth and production. Only a few quantitative trait loci (QTLs) have been identified by bi-parental population. More genes or QTLs are required to dissect the genetic architecture of rice rooting ability.Results To characterize the genetic basis for rice rooting ability, we used a natural rice population, genotyped by a 90K single nucleotide polymorphism (SNP) array, to identify the loci associated with rooting-related traits through the genome-wide association study (GWAS). Population structure analysis divided the natural population into two subgroups: indica and japonica. We measured four traits for evaluating rice rooting ability, namely root growth ability (RGA), maximum root length (MRL), root length (RL), and root number (RN). Combined with the association study in three panels consisting of one for the full population, one for indica, and one for japonica, 32 SNPs associated with rooting ability-related traits were identified. Through comparison of the relative expression levels and DNA sequences between germplasms with extreme phenotypes, results showed that LOC_Os05g11810 had non-synonymous variations at the coding region, which may cause differences in root number, and that the expression levels of LOC_Os04g09900 and LOC_Os04g10060 are closely associated with root length variation.Conclusions The goal of our research was to improve understanding of the genetic basis of rice rooting ability and provide useful molecular markers and germplasms for rice root breeding.

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SLL1, which encodes a member of the stearoyl-acyl carrier protein fatty acid desaturase family, is involved in cell elongation in lateral roots via regulation of fatty acid content in rice

Cited by 17 publications

References 30 publications

Non-Catalytic Subunits Facilitate Quaternary Organization of Plastidic Acetyl-CoA Carboxylase

Non-Catalytic Subunits Facilitate Quaternary Organization of Plastidic Acetyl-CoA Carboxylase

Genome-wide association study of rice rooting ability at the seedling stage

Genome-Wide Association Study of Rice Rooting Ability at the Seedling Stage

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