Slide-agglutination for rapid serological typing of <i>Treponema hyodysenteriae</i>

Hampson, David J.

doi:10.1017/s0950268800067601

Cited by 14 publications

(15 citation statements)

References 9 publications

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“…The plates were placed in anaerobic jars (BBL) under an atmosphere of 94% N2 and 6% CO2 at 37°C for 5 days, and any strongly haemolytic spirochaetes subcultured, grown in Kunkle's broth medium [20], tested for indole production by addition of Kovac's reagent after extraction with zylene, and subjected to slide agglutination to determine serotype [22].…”

Section: Isolation Of Spirochaetesmentioning

confidence: 99%

Pigs experimentally infected withSerpulina hyodysenteriaecan be protected from developing swine dysentery by feeding them a highly digestible diet

1996

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SUMMARYWeaner pigs (n = 72) were fed 1 of 4 diets. These were based on either cooked rice and animal protein, cooked rice and lupin, wheat and lupin, or wheat and animal protein. Twenty-six of the pigs were slaughtered after 1 month. Those fed the highly digestible cooked rice and animal protein diet had drier colonic contents and faeces, lighter large intestines, and the contents of their large intestines had increased pH values and decreased total VFA concentrations. The other 46 pigs were orally challenged with broth cultures of Serpulina hyodysenteriae, and were monitored for faecal excretion of the spirochaetes, and for the development of swine dysentery (SD). None of 18 pigs fed the cooked rice and animal protein diet developed colonic changes or disease, whereas most pigs on the other diets developed mucohaemorrhagic colitis and dysentery. The reduced fermentation that occurred in the large intestines of pigs fed cooked rice and animal protein was associated with a subsequent failure of colonization by S. hyodysenteriae, and resultant protection against SD.

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Section: Isolation Of Spirochaetesmentioning

confidence: 99%

Pigs experimentally infected withSerpulina hyodysenteriaecan be protected from developing swine dysentery by feeding them a highly digestible diet

1996

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“…hyodysenteriae has been classified into serogroups based on the reactivity of lipooligosaccharide antigens with antisera raised against whole cells (1, 6-9, 22, 24). At least nine serogroups, designated A through I, with unique serovars within the serogroups, have been proposed (6,7,9). Serogroups A through D are represented by S. hyodysenteriae strains which have been identified by Baum and Joens (1) as type strains for serotypes 1 through 4.…”

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confidence: 99%

Immunoblot reactivity of polyclonal and monoclonal antibodies with periplasmic flagellar proteins FlaA1 and FlaB of porcine Serpulina species

Fisher

Duhamel

Westerman

et al. 1997

Clin Diagn Lab Immunol

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The periplasmic-flagellum (PF) proteins of Triton X-100-soluble and Triton X-100-insoluble sodium dodecyl sulfate-treated fractions from reference and field strains of Serpulina hyodysenteriae, Serpulina innocens, and Serpulina pilosicoli were characterized by Western blotting with a rabbit polyclonal antibody (PAb) specific for the 44-kDa PF sheath protein of S. hyodysenteriae (Z. Li, F. Dumas, D. Dubreuil, and M. Jacques, J. Bacteriol. 175:8000-8007, 1993) and a murine monoclonal antibody (MAb), designated 7G2, specific for the PF core FlaB proteins of S. hyodysenteriae. The MAb 7G2 reacted with a conserved epitope present in the 37-, 34-, and 32-kDa PF core FlaB proteins of all Serpulina species. This suggested that the core FlaB proteins are conserved among porcine Serpulina species. An immunoreactive band of approximately 44 kDa was present with all S. hyodysenteriae, S. innocens, and S. pilosicoli strains that were reacted with the PAb. The specificities of the PAb and the MAb for the FlaA1 and FlaB proteins of Serpulina species were confirmed by N-terminal amino acid sequencing of 44-and 37-kDa proteins, respectively, of S. hyodysenteriae and S. pilosicoli. Results from this study provide further evidence that the 44-kDa protein FlaA1 and the 37-, 34-, and 32-kDa FlaB proteins are conserved among porcine Serpulina species.

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“…Lyson and Lemcke (16) used a fluorescence antibody test with absorbed antiserum for identification of S. hyodysenteriae. Other workers also used cross-absorbed sera in a slide agglutination test (5,14). Each procedure has merits, but none has been free from problems, mainly due to cross-reactivity.…”

Section: Discussionmentioning

confidence: 99%

“…Both these programs require an extensive knowledge of the different serotypes existing in a particular region, as this organism is known to be antigenically heterogenous with different serotypes showing different geographical distributions (5, 14, 15). Several tests have been used to differentiate S. hyodysenteriae from S. innocens and to serotype S. hyodysenteriae isolates (1,5,14).…”

mentioning

confidence: 99%

Evaluation of microagglutination test for differentiation between Serpulina (Treponema) hyodysenteriae and S. innocens and serotyping of S. hyodysenteriae

Diarra

Mittal

1994

J Clin Microbiol

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Swine dysentery is a mucohemorrhagic diarrheal disease caused by the anaerobic spirochete Serpulina hyodysenteriae. At present, the serotyping is done by immunodiffusion testing with lipopolysaccharide (LPS) extract as antigen and rabbit hyperimmune sera produced against different serotypes of S. hyodysenteriae. Since the preparation of LPS is time-consuming and requires a large quantity of bacteria, it is desirable to use a serotyping method which does not require the extraction of LPS. In the present investigation, microagglutination was evaluated by using both formalinized wholeand boiled-cell suspensions as antigens and rabbit hyperimmune sera produced against formalinized whole-cell suspensions of reference strains of S. hyodysenteriae and S. innocens B256. Use of boiled cell suspension as antigen permitted the differentiation between isolates of S. hyodysenteriae and S. innocens as well as serotyping of S. hyodysenteriae strains accurately. A total of 18 isolates were identified as S. hyodysenteriae, and 3 isolates were identified as S. innocens. The microagglutination test was found specific, sensitive, and easy to perform; thus, it was judged suitable for routine identification and serotyping of S. hyodysenteriae isolates.

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Slide-agglutination for rapid serological typing of Treponema hyodysenteriae

Cited by 14 publications

References 9 publications

Pigs experimentally infected withSerpulina hyodysenteriaecan be protected from developing swine dysentery by feeding them a highly digestible diet

Pigs experimentally infected withSerpulina hyodysenteriaecan be protected from developing swine dysentery by feeding them a highly digestible diet

Immunoblot reactivity of polyclonal and monoclonal antibodies with periplasmic flagellar proteins FlaA1 and FlaB of porcine Serpulina species

Evaluation of microagglutination test for differentiation between Serpulina (Treponema) hyodysenteriae and S. innocens and serotyping of S. hyodysenteriae

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