SLERT Regulates DDX21 Rings Associated with Pol I Transcription

Xing, Yu-Hang; Yao, Run-Wen; Zhang, Yang; Guo, Chunjie; Jiang, Shan; Xu, Guang; Dong, Rui; Yang, Li

doi:10.1016/j.cell.2017.04.011

Cited by 209 publications

(189 citation statements)

References 38 publications

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“…Several recent studies used this approach along with DNA colocalization to define the contributions of lncRNAs to ribosomal DNA differential expression. 49 Probes for single-molecule FISH can be designed according to Raj Laboratory protocol. 47 Another alternative is RNAscope—a novel multiplex nucleic acid in situ hybridization technology that has been used to image lncRNAs.…”

Section: Approach To Lncrna Validationmentioning

confidence: 99%

Long Noncoding RNA Discovery in Cardiovascular Disease

Sallam

Sandhu

Tontonoz

2018

Circulation Research

214

139

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Despite significant improvements during the past 3 decades, cardiovascular disease remains a leading worldwide health epidemic. The recent identification of a fascinating group of mediators known as long noncoding RNAs (lncRNAs) has provided a wealth of new biology to explore for cardiovascular risk mitigation. lncRNAs are expressed in a highly context-specific fashion, and multiple lines of evidence implicated them in diverse biological processes. Indeed, abnormalities of lncRNAs have been directly linked with human ailments, including cardiovascular biology and disease. Of particular interest to the cardiovascular research community, dysregulation in lncRNA regulatory circuits have been associated with cardiac pathological hypertrophy, vascular disease, cell fate programming and development, atherosclerosis, dyslipidemia, and metabolic syndrome. Although techniques in interrogating noncoding RNAs are rapidly evolving, a major challenge in studying lncRNAs remains navigating through multiple technical constraints. In this review, we provide a road map for lncRNA discovery and interrogation in biological systems relevant to cardiovascular disease and highlight approaches to decipher their modes of action.

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Section: Approach To Lncrna Validationmentioning

confidence: 99%

Long Noncoding RNA Discovery in Cardiovascular Disease

Sallam

Sandhu

Tontonoz

2018

Circulation Research

214

139

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“…tRSA RNA pulldown assays were performed according to previous report [75] with some modifications. The corresponding RNA products were in vitro-transcribed using the TranscriptAid T7 High Yield Transcription Kit (Thermo Fisher Scientific).…”

Section: Uv-rna Immunoprecipitationmentioning

confidence: 99%

An LTR retrotransposon‐derived lnc RNA interacts with RNF 169 to promote homologous recombination

Deng

Wang

et al. 2019

EMBO Reports

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LTR retrotransposons are abundant repetitive elements in the human genome, but their functions remain poorly understood. Here, we report the function and regulatory mechanism of an ERV‐9 LTR retrotransposon‐derived lncRNA called p53‐regulated lncRNA for homologous recombination (HR) repair 1 (PRLH1) in human cells. PRLH1 is highly expressed in p53‐mutated hepatocellular carcinoma (HCC) samples and promotes cell proliferation in p53‐mutated HCC cells, and its transcription is promoted by NF‐Y and suppressed by p53. Mechanistically, PRLH1 specifically binds to an uncharacterized domain of RNF169 through two GCUUCA boxes in its 5′ terminal region to form a DNA repair complex that supplants 53BP1 at double‐strand break (DSB) sites and then promotes the initiation of HR repair. Notably, PRLH1 is essential for the stabilization of RNF169, acting as an RNA platform to recruit and assemble HR protein factors. This study characterizes PRLH1 as a novel HR‐promoting factor and provides new insights into the function and mechanism of LTR retrotransposon‐derived lncRNAs.

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“…Interestingly, most of these cases occur in the region shown to be critical for the Prader‐Willi Syndrome (PWS), and may be related to the pathogenesis of this disease. Another sno‐lncRNA, called SLERT was recently shown to act in regulation of RNA Polymerase I activity though binding DDX21 . In the PWS region, there are also transcripts that are 5′ snoRNA capped and 3′ polyadenylated (SPAs).…”

Section: Small Rnas Related To Termini Of Long Noncoding Rnasmentioning

confidence: 99%

Interactions between short and long noncoding RNAs

Ulitsky

2018

FEBS Letters

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It is now evident that noncoding RNAs play key roles in regulatory networks determining cell fate and behavior, in a myriad of different conditions, and across all species. Among these noncoding RNAs are short RNAs, such as MicroRNAs, snoRNAs, and Piwi-interacting RNAs, and the functions of those are relatively well understood. Other noncoding RNAs are longer, and their modes of action and functions are also increasingly explored and deciphered. Short RNAs and long noncoding RNAs (lncRNAs) interact with each other with reciprocal consequences for their fates and functions. LncRNAs serve as precursors for many types of small RNAs and, therefore, the pathways for small RNA biogenesis can impinge upon the fate of lncRNAs. In addition, lncRNA expression can be repressed by small RNAs, and lncRNAs can affect small RNA activity and abundance through competition for binding or by triggering small RNA degradation. Here, I review the known types of interactions between small and long RNAs, discuss their outcomes, and bring representative examples from studies in mammals.

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SLERT Regulates DDX21 Rings Associated with Pol I Transcription

Cited by 209 publications

References 38 publications

Long Noncoding RNA Discovery in Cardiovascular Disease

Long Noncoding RNA Discovery in Cardiovascular Disease

An LTR retrotransposon‐derived lnc RNA interacts with RNF 169 to promote homologous recombination

Interactions between short and long noncoding RNAs

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