Fatigue is a critical issue for nurses that may lead to medical errors, degradation in performance, decreased mental acuity and social problems. Poor sleep quality is also a contributing factor in fatigue that nurses experience. Shift work and high workloads are two factors that caused poor sleep quality of nurses. The aim of the present study was to investigate the relationship between sleep quality and perceived fatigue among nurses at the Cibinong Regional Public Hospital, Bogor District. Cross-sectional study was conducted from January 1, 2017 to April 30, 2017 using a self-reported questionnaire of the Pittsburgh Sleep Quality Index (PSQI) and Subjective Self Rating Test from Industrial Fatigue Research Committee (IFRC) of nurses at Cibinong Regional Public Hospital with 179 nurses. The chi-square test was used to analyze the relationship between sleep quality and perceived fatigue. The results showed that 57 percent of nurses had poor sleep quality. Perceived fatigue measured by subjective self-rating test showed that the majority of nurses had low levels of perceived fatigue (47.5%). Only 8.9 percent of nurses had high levels of perceived fatigue. The chi-square test showed a significant relationship between sleep quality and level of perceived fatigue (p-value = 0.001). Analysis of Odd Ratio showed that nurses who had poor sleep quality had a risk about 2.9 times to experience fatigue, compared with nurses who had good sleep quality. It can be concluded that poor sleep quality has a greater risk of causing fatigue in the nurses. So, the effort of setting the time and shift work is very important to improve the sleep quality of nurses and reduce the risk of work fatigue.