Sleep disruption, oxidative stress, and aging: New insights from fruit flies

Cirelli, Chiara

doi:10.1073/pnas.0606652103

Cited by 27 publications

(20 citation statements)

References 24 publications

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“…Although the insomnia patient is frequently advised that lack of sleep is not harmful or life-threatening, studies in Drosophila demonstrate that sleep loss can affect lifespan, aging, and gene expression (Cirelli, 2006;Koh et al, 2006). By extension, these findings imply that the impact of sleep loss in humans may have greater physiological significance than has been previously appreciated.…”

Section: Assessing the Effects Of Sleep Lossmentioning

confidence: 99%

“…The fruit fly, D. melanogaster, has been proposed as a model for the study of mammalian sleep, and the similarities between characteristics of sleep in the fruit fly and in mammalian species are striking (Hendricks et al, 2000b;Cirelli, 2006). The well-studied genetic mapping, the short life cycle which permits rapid assessment of genetic manipulations in subsequent generations, and the relative ease of maintaining large experimental colonies make the fruit fly an excellent candidate for studying the molecular and genetic aspects of sleep.…”

Section: Insectsmentioning

confidence: 99%

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The phylogeny of sleep

Hartse¹

2011

Handbook of Clinical Neurology

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Section: Assessing the Effects Of Sleep Lossmentioning

confidence: 99%

Section: Insectsmentioning

confidence: 99%

The phylogeny of sleep

Hartse¹

2011

Handbook of Clinical Neurology

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“…Flies share with mammals a similar sleep regulatory mechanism that involves two relatively independent processes: the circadian system that is responsible for consolidating sleep during the night and the homeostatic system that is responsible for wakefulness and sleep drive. Longer waking periods lead to longer and more intense sleep periods [2]. Sleep regulation in Drosophila melanogaster is anatomically located in the mushroom body, known to be involved in learning and memory [3], while approximately 150 clock neurons in the central nervous system are involved in setting circadian rhythms.…”

Section: Introductionmentioning

confidence: 99%

Regulation of Sleep by Neuropeptide Y-Like System in Drosophila melanogaster

Yang

Zhang

et al. 2013

PLoS ONE

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Sleep is important for maintenance of normal physiology in animals. In mammals, neuropeptide Y (NPY), a homolog of Drosophila neuropeptide F (NPF), is involved in sleep regulation, with different effects in human and rat. However, the function of NPF on sleep in Drosophila melanogaster has not yet been described. In this study, we investigated the effects of NPF and its receptor-neuropeptide F receptor (NPFR1) on Drosophila sleep. Male flies over-expressing NPF or NPFR1 exhibited increased sleep during the nighttime. Further analysis demonstrated that sleep episode duration during nighttime was greatly increased and sleep latency was significantly reduced, indicating that NPF and NPFR1 promote sleep quality, and their action on sleep is not because of an impact of the NPF signal system on development. Moreover, the homeostatic regulation of flies after sleep deprivation was disrupted by altered NPF signaling, since sleep deprivation decreased transcription of NPF in control flies, and there were less sleep loss during sleep deprivation and less sleep gain after sleep deprivation in flies overexpressing NPF and NPFR1 than in control flies, suggesting that NPF system auto-regulation plays an important role in sleep homeostasis. However, these effects did not occur in females, suggesting a sex-dependent regulatory function in sleep for NPF and NPFR1. NPF in D1 brain neurons showed male-specific expression, providing the cellular locus for male-specific regulation of sleep by NPF and NPFR1. This study brings a new understanding into sleep studies of a sexually dimorphic regulatory mode in female and male flies.

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“…In addition, the ability to compensate for sleep loss after prolonged waking is diminished (Bonnet and Rosa, 1987; Carskadon and Dement, 1985). Other experimental models including rodents (Shiromani et al, 2000), flies (Cirelli, 2006), and zebrafish (Zhdanova et al, 2008) also exhibit age-related alterations in sleep. In aged rodents (Shiromani et al, 2000), the inability to compensate for sleep loss is accompanied by reductions in protein/mRNA (Cunha et al, 1995; Sperlagh et al, 1997) and/or alterations in protein function (Basheer et al, 2005) and protein profiles (Pawlyk et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Protein expression is altered during spontaneous sleep in aged Sprague Dawley rats

Vázquez¹,

Hall²,

Greco³

2009

Brain Research

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Age-related changes in brain function include those affecting learning, memory, and sleep-wakefulness. Sleep-wakefulness is an essential behavior that results from the interaction of multiple brain regions, peptides and neurotransmitters. The biological function(s) of sleep, however, remains unknown, due to a paucity of information available at the cellular level. Aged rats exhibit alterations in the circadian and homeostatic influences associated with sleep-wake regulation. We recently showed that alterations in cortical profiles occur after timed bouts of spontaneous sleep in young rats. Examination of the cellular response to sleep-wake in old rats may thus provide insight(s) into the biological function(s) of sleep. To test this hypothesis, we monitored cortical profiles in the frontal cortex of young and old Sprague-Dawley rats after timed bouts of spontaneous sleep-wake behavior. Proteins were separated by two-dimensional electrophoresis (2-DE), visualized by fluorescent staining, imaged, and analyzed as a function of behavioral state and age. Old rats showed a 6-fold increase in total protein expression, independent of the behavioral state at sacrifice. When analyzed according to age and behavioral state, there was a decrease (~46%) in the number of phospho-spots present during SWS in aged animals. SWS-associated spots present only in old animals were associated with multiple functions including vesicular transport, cell signaling, oxidation state, cytoskeletal support, and energy metabolism. These data suggest that the intracellular response to the signaling associated with spontaneous sleep is affected by age and is consistent with the idea that the ability of sleep to fulfill its’ function(s) may become diminished with age.

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Sleep disruption, oxidative stress, and aging: New insights from fruit flies

Cited by 27 publications

References 24 publications

The phylogeny of sleep

The phylogeny of sleep

Regulation of Sleep by Neuropeptide Y-Like System in Drosophila melanogaster

Protein expression is altered during spontaneous sleep in aged Sprague Dawley rats

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