Sleep disruption increases seizure susceptibility: Behavioral and EEG evaluation of an experimental model of sleep apnea

Hrnčić, Dragan; Grubač, Željko; Rašić-Marković, Aleksandra; Šutulović, Nikola; Šušić, Veselinka; Bjekić-Macut, Jelica; Stanojlović, Olivera

doi:10.1016/j.physbeh.2015.12.016

Cited by 10 publications

(10 citation statements)

References 42 publications

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“…Additional tests in recovery phase also confirm these findings. Taking into account these results together with our previous experience with this methodology [3], we did not repeat sleep recordings in the current study in order to keep animal number as low as possible for ethical reasons.…”

Section: Discussionmentioning

confidence: 99%

“…The treadmill apparatus ( NeuroSciLaBG-Treadmill , Elunit , Serbia) used in this study consists of a conveyor belt with motion programmed in advance with the customized control program, as reported in our previous study [3]. The treadmill activity was defined by ON mode (belt moving alternatively forwards or backwards) at the speed of 0.02 m/s and OFF mode at the speed of 0.00 m/s.…”

Section: Methodsmentioning

confidence: 99%

“…Namely, inadequacy of the quantity and the architecture of sleep are associated with numerous diseases ranging from cardiovascular to neurological (e.g. epilepsy) and psychiatric disorders [3–5]. Recent evidence suggested a bidirectional relationship between psychiatric disorders and sleep disturbances, in which they reinforce each other and share common and overlapping mechanisms [6,7].…”

Section: Introductionmentioning

confidence: 99%

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Short-term sleep fragmentation enhances anxiety-related behavior: The role of hormonal alterations

et al. 2019

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Introduction The neuroendocrine background of acute sleep fragmentation in obstructive sleep apnea and sleep fragmentation involvement in psychiatric comorbidities, common in these patients, are still largely unknown. The aim of this study was to determine the effects of short-term experimental sleep fragmentation on anxiety -like behavior and hormonal status in rats. Methods Male rats were adapted to treadmill (ON and OFF mode with belt speed set on 0.02m/s and 0.00m/s) and randomized to: 1) treadmill control (TC, only OFF mode); 2) motion, activity control (AC, 10min ON and 30min OFF mode) and 3) sleep fragmentation (SF, 30s ON and 90s OFF mode) group. Six hours later, the animals were tested in the open field, elevated plus maze and light/dark test (n = 8/group). Testosterone, estradiol, progesterone and corticosterone were determined in separate animal cohort immediately upon sleep fragmentation (n = 6/group). Results SF rats showed decreased rearings number, decreased time spent in the central area and increased thigmotaxic index compared to TC and AC rats in the open field test. Similarly, increased anxiety upon sleep fragmentation was observed in the elevated plus maze and the light/dark test. Significantly lower testosterone, estradiol and progesterone levels were determined in SF in comparison to AC and TC groups, while there was no significant difference in the levels of corticosterone. Conclusion Short term sleep fragmentation enhances anxiety-related behavior in rats, which could be partly mediated by the observed hormonal changes presented in the current study in form of testosterone, estradiol and progesterone depletion.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Short-term sleep fragmentation enhances anxiety-related behavior: The role of hormonal alterations

et al. 2019

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“…The reduction of factors which may promote seizures, such as sleep fragmentation (Rajna and Veres, 1993;Frucht et al, 2000), could lead to new and more effective treatment strategies. In this study we used the lindane model of experimentally induced generalized seizures, as described earlier (Vučević et al, 2008;Hrnčić et al, 2016), because lindane induced seizures are refractory to numerous classical antiepileptic drugs and therefore believed to be suitable for exploration of refractory epilepsy (Vučević et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Experimental chronic sleep fragmentation alters seizure susceptibility and brain levels of interleukins 1β and 6

Grubač

Šutulović

Jerotić

et al. 2021

Acta Neurobiologiae Experimentalis

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Brain hyperexcitability in sleep apnea is believed to be provoked by hypoxemia, but sleep fragmentation can also play a significant role. Sleep fragmentation can trigger inflammatory mechanisms. The aim of this research was to investigate the effects of chronic sleep fragmentation on seizure susceptibility and brain cytokine profile. Chronic sleep fragmentation in male rats with implanted EEG electrodes was achieved by the treadmill method. Rats were randomized to: treadmill control (TC); activity control (AC) and sleep fragmentation (SF) group. Convulsive behavior was assessed 14 days later by seizure incidence, latency time and seizure severity during 30 min following lindane administration. The number and duration of EEG ictal periods were determined. Levels of IL-1β and IL-6 were measured in the animals' serum and brain structures (hippocampus, thalamus and cerebral cortex), in separate rat cohort that underwent the same fragmentation protocol except lindane administration. Incidence and severity of seizures were significantly increased, while latency was significantly decreased in SF+L compared with TC+L group. Seizure latency was also significantly decreased in SF+L compared to AC+L group. The number and duration of ictal periods were increased in the SF+L compared to the AC+L group. IL-1β was significantly increased in the thalamus, cortex and hippocampus in the SF compared to the AC and TC groups. IL-6 was statistically higher only in the cortex of SF animals, while in the thalamic or hippocampal tissue, no difference was observed between the groups. It could be concluded that fourteen-day sleep fragmentation increases seizure susceptibility in rats and modulates brain production of IL-1β and IL-6.

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“…The most common among them are: fever in children, tricyclic antidepressants, theophylline, drugs intoxications and drugs abuse, acute neurological disorders and infections (meningitis, encephalitis, stroke, head injury, brain abscess, etc. ), alcoholism, metabolic disorders (hypo/hyperglycemia, hypocalcaemia) 7 , derivative of phencyclidine-metaphit 8 , scabicides-lindane 9, 10 , sleep disturbances [11][12][13] , hyperhomocysteinemia 14 , visual stimuli 15 , stress 16 , the menstrual cycle 17 , a specific diet regimes 18,19 , vascular abnormalities, stroke sequelae and subarachnoid hemorrhage 20 . Experimental models of epilepsy are significant for resolving the mechanisms of epileptogenesis and play very important role in new antiepileptic drugs development.…”

Section: Introductionmentioning

confidence: 99%

The central nervous system is not imunoprivileged: Inflammation and epileptogenesis

Hrnčić¹,

Šutulović²,

Grubač³

et al. 2018

VOJNOSANIT PREGL

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Sleep disruption increases seizure susceptibility: Behavioral and EEG evaluation of an experimental model of sleep apnea

Cited by 10 publications

References 42 publications

Short-term sleep fragmentation enhances anxiety-related behavior: The role of hormonal alterations

Short-term sleep fragmentation enhances anxiety-related behavior: The role of hormonal alterations

Experimental chronic sleep fragmentation alters seizure susceptibility and brain levels of interleukins 1β and 6

The central nervous system is not imunoprivileged: Inflammation and epileptogenesis

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