SKRINING FITOKIMIA EKSTRAK ETANOL Ulva lactuca L. DENGAN METODE KROMATOGRAFI LAPIS TIPIS

Widyaningsih, Wahyu; Pramono, Suwijiyo; Widyarini, Sitarina; Sugiyanto, Sugiyanto

doi:10.12928/mf.v13i2.7772

Cited by 5 publications

(4 citation statements)

References 8 publications

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“…The results showed that flavonoids, saponins, tannins, and phenols had weak activity (+), while alkaloids and steroids/terpenoids were classified as moderately active (++). This phytochemical screening was different from Widyaningsih et al (2016), that Ulva lactuca ethanolic extract from Drini Waters, Gunung Kidul, Yogyakarta-Indonesia, contained flavonoids and alkaloids with weak activity (+), while saponins, steroids and tannins had no activity (-). In addition, phytochemical content of the sample from Cemara Sewu waters, Yogyakarta, contained alkaloids, flavonoids, saponins, tannins, phenols, and steroids with weak activity (+) (Panjaitan et al 2022).…”

Section: Qualitative Screening and Quantitative Phytochemical Contentsmentioning

confidence: 58%

Potential use of phytochemical from ethanolic extract of green seaweed Ulva reticulata in aquaculture

TARIGAN,

SUDRAJAT,

ARFAH

et al. 2024

Biodiversitas

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Abstract. Tarigan N, Sudrajat AO, Arfah H, Alimuddin A, Wahjuningrum D. 2023. Potential use of phytochemical from ethanolic extract of green seaweed Ulva reticulata in aquaculture. Biodiversitas 24: 6868-6879. Ulva reticulata is green seaweed with potential phytochemical properties in the aquaculture sector. Therefore, this study aims to determine phytochemical components and evaluate the toxicity of Ulva reticulata ethanolic extract on aquatic animals. The primary constituents in the sample were extracted using the maceration method with 96% ethanol. The antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, while toxicity level was determined using the Brine Shrimp Lethal Test (BSLT) method at concentrations of 0, 10, 100, 300, 500, 1000, and 2000 mg L-1. The results showed that Ulva reticulata ethanolic extract was qualitatively composed of alkaloids, flavonoids, saponins, tannins, phenols, and steroids with mid (++), low (+), low (+), low (+), low (+), and mid (++) activity levels, respectively. Based on quantitative analysis, the sample was composed of sitosterol (19.01 mg g-1), stigmasterol (35.00 mg g-1), saponins (17.04 mg g-1), flavonoids (82.16 mg QE 100 g-1), total phenols (144.00 g QE 100 g-1), and tannins (49.03 mg g-1). Furthermore, Ulva reticulata ethanolic extract had a strong antioxidant level with a 50% inhibitory concentration (IC50) of 53.00 ppm. The toxicity test showed that the 50% lethal concentration (LC50) of the sample was 481.86 mg L-1. These results showed that Ulva reticulata ethanolic extract was a non-toxic material with a high potential for phytochemical properties that could be beneficial for fish growth, reproduction, and health.

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Section: Qualitative Screening and Quantitative Phytochemical Contentsmentioning

confidence: 58%

Potential use of phytochemical from ethanolic extract of green seaweed Ulva reticulata in aquaculture

TARIGAN,

SUDRAJAT,

ARFAH

et al. 2024

Biodiversitas

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“…Phenolic compounds can reduce the risk of several chronic diseases because they have inflammatory, antioxidant, carcinogenic detoxification, and anti-cholesterol properties (Wan et al 2021). Bioactive compounds can be determined through phytochemical tests and are important in antioxidant activity (Widyaningsih et al 2016;Hasby et al 2020).…”

Section: Bioactive Componentsmentioning

confidence: 99%

Evaluation of phytochemical composition and metabolite profiling of macroalgae Caulerpa taxifolia and C. peltata from the Banda Aceh coast, Indonesia

AKBAR,

HASAN,

AFRIANI

et al. 2023

Biodiversitas

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Abstract. Akbar SA, Hasan M, Afriani S, Nuzlia C. 2023. Evaluation of phytochemical composition and metabolite profiling of macroalgae Caulerpa taxifolia and C. peltata from the Banda Aceh coast, Indonesia. Biodiversitas 24: 5283-5292. The study of seaweeds to extract their bioactive compounds has become increasingly important from the standpoint of broader applications. This study aimed to examine the metabolite profiles and phytochemical content of Caulerpa peltata and Caulerpa taxifolia along the coast of Banda Aceh in Indonesia. Readers interested in learning more about the biological activities of C. peltata and C. taxifolia can refer to the material gathered from this study. The three steps proposed by this study were phytochemical composition, antioxidant scavenging, and in vivo metabolite profiling by the use of Gas Chromatography-Mass Spectrometry (GC-MS), Fourier Transform Infrared (FTIR) characterization, and the 2,2-diphenylpicrylhydrazyl (DPPH) test. Using DPPH to measure antioxidant activity, both extracts demonstrated greater scavenging capability, with inhibition values of 42.59% and 39.81%, respectively. In both macroalgae, the GC-MS methodology has shown to be a quick, sensitive, and trustworthy way to monitor individual components and the entire chemical composition. In C. taxifolia, there are 29 phytochemical compounds, whereas in C. peltata, there are 26 types. The ethanol extract of the macroalgae C. taxifolia and C. peltata was then subjected to an FTIR spectrum. These findings at 3352 cm-1, 1654 cm-1, and 1019 cm-1 proved that alkanes, alkenes, and hydroxyl groups were present, corresponding to several compounds in the GC-MS measurement results. Caulerpa taxifolia and C. peltata are potential antibacterial agents; the bioactive compounds for this activity are Heptadecane, Hexadecanoic acid, and other ester derivatives.

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“…The extraction method used was maceration (Widyaningsih et al, 2016). First, the raw green algae were ground into powder, then 500 g of the powder was weighed and soaked for three days in 1000 ml of 96% ethanol.…”

Section: Green Algae Extractionmentioning

confidence: 99%

“…Green algae (Ulva lactuca L) have several active substances with the potential to heal burns. For instance, (Widyaningsih et al, 2016), using thin-layer chromatography, identified the presence of melatonin green algae extracts, while (Dewi, 2018) found vitamin C in this plant. Melatonin can suppress inflammatory responses and increase angiogenesis and collagen synthesis ( L i u e t a l .…”

Section: Introductionmentioning

confidence: 99%

Burn wound healing activity of ethanol extract gel of Green Algae (Ulva lactuca L) in mice

et al. 2022

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Recently, burn injuries have become a major cause of morbidity and mortality in low-middleincome countries. Burn injuries are tissue loss caused by contact with heat sources such as hot water, fire, chemicals, electricity, and radiation. According to the WHO Global Burden Disease, in 2017 an estimated 180.000 people died from burn injuries. Ethanol extract of green algae (EEGA) contains compounds that potentially heal burn injuries. The research was conducted to obtain a gel formulation from EEGA and test its burn wound healing properties in mice (Mus musculus). Forty-five mice were divided into five groups: I (negative control), II (treated with gel base), III (positive control, Bioplacenton gel), IV (5% EEGA gel), and V (10% EEGA gel). Wound diameters and description scores were observed every fourth, seventh, and fourteenth day, and the derived data were analyzed in the SPSS program with the one-way analysis of variance (ANOVA) and least significance difference (LSD) test. The results indicate that EEGA can be formulated into gels with physical properties compliant with the requirements of the dosage form. Further, it was found that Groups IV and V showed significant reductions in wound description scores and diameters (p<0.05). In conclusion, gels containing 5% and 10% EEGA possess burn wound healing properties.

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SKRINING FITOKIMIA EKSTRAK ETANOL Ulva lactuca L. DENGAN METODE KROMATOGRAFI LAPIS TIPIS

Cited by 5 publications

References 8 publications

Potential use of phytochemical from ethanolic extract of green seaweed Ulva reticulata in aquaculture

Potential use of phytochemical from ethanolic extract of green seaweed Ulva reticulata in aquaculture

Evaluation of phytochemical composition and metabolite profiling of macroalgae Caulerpa taxifolia and C. peltata from the Banda Aceh coast, Indonesia

Burn wound healing activity of ethanol extract gel of Green Algae (Ulva lactuca L) in mice

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