Skin Structure and Biology

Woo, Wei‐Meng

doi:10.1002/9783527814633.ch1

Cited by 12 publications

(12 citation statements)

References 42 publications

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“…An essential part of these cells expressed cytokeratin subunits and formed granular structures similar to the sweat or sebaceous glands (22). In the present study, BM-MSCs flattened and formed a single layer after being placed on the anterior epithelium matrix.…”

Section: Resultssupporting

confidence: 52%

Preparation of three-dimensional matrices of human gingival tissue and comparison of induction of mesenchymal and blastema stem cell behaviors in prepared scaffolds

Zhao

Zhu³

et al. 2022

Cell Mol Biol (Noisy-le-grand)

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This study aimed to compare the behavior of rat bone marrow mesenchymal stem cells and rabbit auricle blastema cells implanted in non-cellular gingival tissue scaffold of human. In this regard, the tissues obtained from gingival surgeries in the dental clinic were de-cellulated using two detergents of sodium dodecyl sulfate and triton 100-X. After washing and sterilization, they were used as a scaffold for culture with bone marrow mesenchymal stem cells of rats. Using light and electron microscopy, these scaffolds were examined before and after 1, 2, and 4 weeks of cell culture. Also, the prepared three-dimensional scaffold was placed in the blastema ring obtained from the rabbit earlobe punch. The samples were evaluated 1, 2, and 4 weeks after culture based on histology techniques. The results showed that the study of scaffolds by electron microscopy showed preserving the epithelium matrix and the collagen fibers in the tissue. Structures similar to the epithelium were created in both samples. In addition, induction of cellular secretion was observed in scaffold cells migrating to the scaffold. In general, scaffolds made from human gums can be a good platform for studying cellular behaviors. Of course, further experiments to determine the nature of differentiated cells can help advance our knowledge of matrix cell interactions.

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Section: Resultssupporting

confidence: 52%

Preparation of three-dimensional matrices of human gingival tissue and comparison of induction of mesenchymal and blastema stem cell behaviors in prepared scaffolds

Zhao

Zhu³

et al. 2022

Cell Mol Biol (Noisy-le-grand)

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“…The dermis is organized by an extracellular matrix (ECM) complex composed of collagen, elastin, and other protein components. Collagen is synthesized by fibroblasts in the dermis, and the ECM complex maintains flexibility, elasticity, and moisture in the skin [ 1 ]. Type 1 collagen is the major collagen protein produced in fibroblasts, accounting for over 90% of collagen [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Withagenin A Diglucoside from Indian Ginseng (Withania somnifera) against Human Dermal Fibroblast Damaged by TNF-α Stimulation

et al. 2022

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Human skin is constructed with many proteins such as collagen and elastin. Collagen and elastin play a key role in providing strength and elasticity to the human skin and body. However, damage to collagen causes various symptoms such as wrinkles and freckles, which suggests that they are important to maintain skin condition. Extrinsic or intrinsic skin aging produces an excess of skin destructive factors such as tumor necrosis factor (TNF)-α, which is a major mediator of the aging process. In aged skin, TNF-α provokes the generation of intracellular ROS (reactive oxygen species). It triggers the excessive secretion of MMP-1, which is a collagen-degrading enzyme that causes the collapse of skin collagen. Therefore, we aimed to search for a natural-product-derived candidate that inhibits the skin damage caused by TNF-α in human dermal fibroblasts. In this study, the protective effect of withagenin A diglucoside (WAD) identified from Withania somnifera against TNF-α-stimulated human dermal fibroblasts is investigated. W. somnifera (Solanaceae), well-known as ‘ashwagandha’, is an Ayurvedic medicinal plant useful for promoting health and longevity. Our experimental results reveal that WAD from W. somnifera suppresses the generation of intercellular ROS. Suppressing intracellular ROS generation inhibits MMP-1 secretion and the collapse of type 1 collagen. The effect of WAD is shown to depend on the inhibition of MAPK phosphorylation, Akt phosphorylation, c-Jun phosphorylation, COX-2 expression, and NF-κB phosphorylation. Further, WAD-depressed expression of the pro-inflammatory cytokines IL-6 and IL-8 triggers various inflammatory reactions in human skin. These findings suggest that WAD has protective effects against skin damage. Accordingly, our study provides experimental evidence that WAD can be a potential agent that can be applied in various industrial fields, such as cosmetics and pharmaceuticals related to skin aging.

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“…A muscle layer can be found adjacent to this layer, which overlies either bony prominences or interior tissues and organs. It is also the site of the formation of certain blood vessels that extend into the dermis [ 31 ].…”

Section: Human Skin Structurementioning

confidence: 99%

Cellular Interaction of Human Skin Cells towards Natural Bioink via 3D-Bioprinting Technologies for Chronic Wound: A Comprehensive Review

Masri

Mazlan

Zulkiflee

et al. 2022

IJMS

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Skin substitutes can provide a temporary or permanent treatment option for chronic wounds. The selection of skin substitutes depends on several factors, including the type of wound and its severity. Full-thickness skin grafts (SGs) require a well-vascularised bed and sometimes will lead to contraction and scarring formation. Besides, donor sites for full-thickness skin grafts are very limited if the wound area is big, and it has been proven to have the lowest survival rate compared to thick- and thin-split thickness. Tissue engineering technology has introduced new advanced strategies since the last decades to fabricate the composite scaffold via the 3D-bioprinting approach as a tissue replacement strategy. Considering the current global donor shortage for autologous split-thickness skin graft (ASSG), skin 3D-bioprinting has emerged as a potential alternative to replace the ASSG treatment. The three-dimensional (3D)-bioprinting technique yields scaffold fabrication with the combination of biomaterials and cells to form bioinks. Thus, the essential key factor for success in 3D-bioprinting is selecting and developing suitable bioinks to maintain the mechanisms of cellular activity. This crucial stage is vital to mimic the native extracellular matrix (ECM) for the sustainability of cell viability before tissue regeneration. This comprehensive review outlined the application of the 3D-bioprinting technique to develop skin tissue regeneration. The cell viability of human skin cells, dermal fibroblasts (DFs), and keratinocytes (KCs) during in vitro testing has been further discussed prior to in vivo application. It is essential to ensure the printed tissue/organ constantly allows cellular activities, including cell proliferation rate and migration capacity. Therefore, 3D-bioprinting plays a vital role in developing a complex skin tissue structure for tissue replacement approach in future precision medicine.

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Skin Structure and Biology

Cited by 12 publications

References 42 publications

Preparation of three-dimensional matrices of human gingival tissue and comparison of induction of mesenchymal and blastema stem cell behaviors in prepared scaffolds

Preparation of three-dimensional matrices of human gingival tissue and comparison of induction of mesenchymal and blastema stem cell behaviors in prepared scaffolds

Protective Effects of Withagenin A Diglucoside from Indian Ginseng (Withania somnifera) against Human Dermal Fibroblast Damaged by TNF-α Stimulation

Cellular Interaction of Human Skin Cells towards Natural Bioink via 3D-Bioprinting Technologies for Chronic Wound: A Comprehensive Review

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