Skin microbiome modulation induced by probiotic solutions

Paetzold, Bernhard; Willis, Jesse R.; Lima, José Pinto de; Knödlseder, Nastassia; Quist, Sven R; Gabaldón, Toni; Güell, Marc

doi:10.1101/423285

Cited by 22 publications

(30 citation statements)

References 36 publications

(13 reference statements)

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“…Yet only a minority of people will develop diseases such as sepsis, pneumonia, or osteomyelitis caused by S. auerus infection (33, 34). Similar phenomenon are observed for Cutibacterium acnes as a contributor to skin acne, which is also a major population in the healthy skin microbiome (35). In this respect, the dynamics of Ea growth and fire blight development appear to follow similar dynamics of other diseases, with a high carrier rate, but lower disease incidence.…”

Section: Discussionsupporting

confidence: 75%

Temporal and spatial dynamics in the apple flower microbiome in the presence of the phytopathogenErwinia amylovora

Cui

Huntley

Zeng

et al. 2020

Preprint

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17Plant microbiomes have important roles in plant health and productivity. However, 18 despite flowers being directly linked to reproductive outcomes, little is known about the 19 microbiomes of flowers and their potential interaction with pathogen infection. Here, we 20 investigated the temporal dynamics and spatial traits of the apple stigma microbiome 21 when challenged with a phytopathogen Erwinia amylovora, the causal agent of fire blight 22 disease. We profiled the microbiome from the stigmas of a single flower, greatly 23 increasing the resolution at which we can characterize shifts in the composition of the 24 microbiome. Individual flowers harbored unique microbiomes at the OTU level. 25However, taxonomic analysis of community succession showed a population gradually 26 dominated by bacteria within the families Enterobacteriaceae and Pseudomonadaceae. 27 Flowers inoculated E. amylovora established large populations of the phytopathogen, 28 with pathogen specific gene counts of >3.0 x 10 7 in 90% of the flowers. Yet, only 42% of 29 inoculated flowers later developed fire blight symptoms. This reveals pathogen amount 30 on the stigma is not sufficient to predict disease outcome. Our data demonstrate that 31 apple flowers represent an excellent model in which to characterize how plant 32 microbiomes establish, develop, and interact with biological processes such as disease 33 progression in an experimentally tractable plant organ.34 35 36 37 38 39 40 Flowers, the reproductive organs of angiosperms, play a critical role in the plant's 41 lifecycle. The most important function of flowers is to provide a mechanism for 42 pollination, the union of sperm contained within pollen, to the ovules contained in the 43 ovary. The fertilized ovules produce seeds that will later germinate to become the next 44 generation of plants. Yet, unlike other vegetative organs such as the roots, stems, and 45 leaves that are present through a large part of the plant's lifecycle, flowers develop on 46 mature plants and are typically present for the limited period during bloom. As such, 47 research characterizing the microbiome of the flower is generally less developed than for 48 other plant organs. 49 Flowers of apple (Malus domestica) have been subject to considerable research 50 attention as they are the direct precursors of apple fruits, one of the most consumed fruits 51 worldwide (1). The ephemeral nature of apple flowers, with mature flowers from petal 52 open to petal fall only lasting for 5-10 days in spring, offers a unique environment in 53 which to study community succession (1, 2). During bloom, petals open up in a relatively 54 short period of time, typically within one day, which exposes the internal flower parts to 55 the environment and microorganisms. Several of these internal flower parts exude various 56 types of nutrient-rich secretions including nectar, stigmatic exudate, and pollen exudate, 57 for the purpose of attracting pollinators, and inducing the germination of pollen grains (1, 58 3). These secretio...

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Section: Discussionsupporting

confidence: 75%

Temporal and spatial dynamics in the apple flower microbiome in the presence of the phytopathogenErwinia amylovora

Cui

Huntley

Zeng

et al. 2020

Preprint

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“…To address this hypothesis, a proof-of-principle study was conducted in healthy participants in different acneprone skin areas to demonstrate that new strains of the resident skin microbiota can be successfully transferred to a recipient. The results of this study demonstrate that the composition of the skin microbiome could be modified at the C. acnes strain level within a few days and no major safety events were identified (13). Of note, in microbiome modulation studies unlike traditional studies,…”

mentioning

confidence: 70%

“…The total number of operational taxonomic units (OTUs) were detected using the software Mothur (University of Michigan, Ann Arbor, MI, USA). The SLST amplification and analysis was performed as described previously (13).…”

Section: Methodsmentioning

confidence: 99%

Safety and Efficacy of Topically Applied Selected Cutibacterium acnes Strains over Five Weeks in Patients with Acne Vulgaris: An Open-label, Pilot Study

Karoglan

Paetzold²,

Lima³

et al. 2019

Acta Derm Venereol

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This open-label, pilot study enrolled men and women (18years) with acne vulgaris. In 14 enrolled patients, skin microbiome composition shifted towards study formulations. No untoward tolerability, visible irritation, or significant flare-ups were observed. Non-inflamed lesions and skin pH were reduced. Modulation of Cutibacterium acnes towards the non-acne causing strains on the skin of patients with acne was safe. C. acnes was formerly thought to be the cardinal cause of acne; however, recently some, but not all, strains of C. acnes were found to be responsible. This microbiome modulation approach as therapy requires validation in future clinical studies. Imbalance in skin microflora, particularly related to certain Cutibacterium acnes strains, may trigger acne. Application of non-acne-causing strains to the skin may modulate the skin microbiome and thereby lead to a reduction in acne. This pilot study evaluates the safety and efficacy of microbiome modulation on acneprone skin. The study had 2 phases: active induction (5% benzoyl peroxide gel, 7 days) and interventional C. acnes strains treatment (5 weeks). Patients were randomized to either topical skin formulations PT1 (2 strains of C. acnes Single Locus Sequence Typing [SLST] type C3 and K8, 50% each) or PT2 (4 strains of C. acnes SLST type C3 [55%], K8 [5%], A5 [30%] and F4 [10%]). Safety and efficacy was evaluated in 14 patients (PT1=8/14, PT2=6/14). Skin microbiome composition shifted towards study formulations. No untoward adverse events, visible irritation, or significant flare-up were observed. Non-inflamed lesions and skin pH were reduced. Comedone counts improved clinically with no deterioration in inflammatory lesions.

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“…As fecal transplant of gut microbiome has been proven with a successful story to treat certain disease that is approved by FDA [6,7], skin microbiome may become an interesting therapeutic option in diseases affecting the skin such as psoriasis or acne vulgaris [8]. Interestingly, the modulation of the skin microbiome composition was reported successful which has been carried out by adding mixtures of different skin microbiome components [8], or also known as bacterial cocktail.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous profiling and cultivation of the skin microbiome of healthy young adult skin for the development of therapeutic agents

Khayyira¹,

Rosdina²,

Irianti

et al. 2020

Heliyon

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Background: Studies on the impact of the skin microbiota on human health have been gaining more attention. Bacteria are associated with various diseases, although certain strains of bacteria, which are known as probiotics, are considered beneficial. Mixtures of several bacteria (bacterial cocktail) isolated from targeted organs have shown promising modulatory activities for use in skin therapeutics. The objectives of this study were to determine and identify the microbial communities on the skin that can potentially be used as probiotics, as determined by bacterial isolation and cultivation, followed by next-generation sequencing (NGS). Results: Samples were collected by swabbing on forehead and cheek skin. Genomic DNA from bacterial swab samples were directly extracted to be further processed into NGS. Cultivation of skin bacteria was carried out in subsequent medium. Thus, around twenty bacterial isolates with different characteristics were selected and identified by both culture-based method and 16sRNA sequencing. We found that Actinobacteria and Firmicutes are the most abundant phylum present on the skin as presented by NGS data, which constitute to 67% and 28.59% of the whole bacterial population, consecutively. However, Staphylococcus hominis, Staphylococcus warneri, and Micrococcus luteus (AN MK968325.1; AN MK968315.1; and MK968318.1 respectively) were able to be obtained in the samples of cultivable, and could be potentially developed as probiotics in skin microbiome therapeutic as well as for postbiotic formulation. Conclusion: Skin microbiome is considered to provide several probiotics for skin therapeutic. However, some opportunistic pathogens were discovered in this study population. Thus, the promising formula of bacterial cocktail for skin microbiome therapeutic must be thoroughly elucidated to avoid unwanted species. Our study is the first human skin microbiome profile of Indonesia resulted from a Next Generation Sequencing as an effort to show a representative of tropical country profile.

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Skin microbiome modulation induced by probiotic solutions

Cited by 22 publications

References 36 publications

Temporal and spatial dynamics in the apple flower microbiome in the presence of the phytopathogenErwinia amylovora

Temporal and spatial dynamics in the apple flower microbiome in the presence of the phytopathogenErwinia amylovora

Safety and Efficacy of Topically Applied Selected Cutibacterium acnes Strains over Five Weeks in Patients with Acne Vulgaris: An Open-label, Pilot Study

Simultaneous profiling and cultivation of the skin microbiome of healthy young adult skin for the development of therapeutic agents

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