Skin corrosion test: a comparison between reconstructed human epidermis and full thickness skin models

Catarino, Carolina Motter; Pedrosa, Tatiana do Nascimento; Pennacchi, Paula Comune; Assis, Sílvia Romano de; Gimenes, Fernanda Raphael Escobar; Consolaro, Márcia Edilaine Lopes; Barros, Sílvia Berlanga de Moraes; Maria‐Engler, Silvya Stuchi

doi:10.1016/j.ejpb.2018.01.002

Cited by 28 publications

(27 citation statements)

References 29 publications

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“…The last one was formed from the undifferentiated cells, with a full metabolic activity, which was lost in later proliferation of the cells and formation of the corneocytes. It is important to mention that macrophage and Langerhans cells were not present, which are the first defensive line of the skin [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

New Sustainable Process for Hesperidin Isolation and Anti-Ageing Effects of Hesperidin Nanocrystals

et al. 2020

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Hesperidin, a secondary orange (Citrus sinensis) metabolite, was extracted from orange bagasse. No organic solvents or additional energy consumption were used in the clean and sustainable process. Hesperidin purity was approximately 98% and had a yield of 1%. Hesperidin is a known supplement due to antioxidant, chelating, and anti-ageing properties. Herein, hesperidin application to eliminate dark eye circles, which are sensitive and thin skin regions, was studied. In addition, the proposed method for its aqueous extraction was especially important for human consumption. Further, the most effective methods for hesperidin nanonization were explored, after which the nanoemulsions were incorporated into a cream formulation that was formulated for a tropical climate. Silky cream formulations (oil in water) were tested in vitro on artificial 3D skin from cultured cells extracted from skin residues after plastic surgery. The proposed in vitro assay avoided tests of the different formulations in human volunteers and animals. It was shown that one of the nanonized hesperidin formulations was the most skin-friendly and might be used in cosmetics.

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Section: Resultsmentioning

confidence: 99%

New Sustainable Process for Hesperidin Isolation and Anti-Ageing Effects of Hesperidin Nanocrystals

et al. 2020

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“…Thus, for phototoxicity prediction, the recommended in vitro tiered strategy, including monolayer fibroblast 3T3 Neutral Red Uptake Phototoxicity (3T3 NRU PT) and reconstructed human epidermis (RHE), allows for the identification of phototoxic potential without animal testing [5,17,18]. Nevertheless, RHE does not present the dermal component, which is essential for many epidermal characteristics and proper skin functionalities, including the improvement of barrier function [19]. In our study, we replaced RHE with reconstructed human skin (RHS) to confirm the ability of the latter to detect the phototoxicity potential.…”

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confidence: 99%

Fucoxanthin for Topical Administration, a Phototoxic vs. Photoprotective Potential in a Tiered Strategy Assessed by In Vitro Methods

et al. 2020

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Fucoxanthin possesses a well-described antioxidant activity that might be useful for human skin photoprotection. However, there is a lack of scientific information regarding its properties when applied onto human skin. Thus, the objective of the present study was to assess the photoprotective and phototoxicity potential of fucoxanthin based on its ultraviolet (UVB 280–320 nm; UVA 320–400 nm) and visible (VIS 400–700 nm) absorption, photostability, phototoxicity in 3T3 mouse fibroblast culture vs. full-thickness reconstructed human skin (RHS), and its ability to inhibit reactive oxygen species formation that is induced by UVA on HaCaT keratinocytes. Later, we evaluated the antioxidant properties of the sunscreen formulation plus 0.5% fucoxanthin onto RHS to confirm its bioavailability and antioxidant potential through the skin layers. The compound was isolated from the alga Desmarestia anceps. Fucoxanthin, despite presenting chemical photo-instability (dose 6 J/cm2: 35% UVA and 21% VIS absorbance reduction), showed acceptable photodegradation (dose 27.5 J/cm2: 5.8% UVB and 12.5% UVA absorbance reduction) when it was added to a sunscreen at 0.5% (w/v). In addition, it increased by 72% of the total sunscreen UV absorption spectra, presenting UV-booster properties. Fucoxanthin presented phototoxic potential in 3T3 fibroblasts (mean photo effect 0.917), but it was non-phototoxic in the RHS model due to barrier function that was provided by the stratum corneum. In addition, it showed a significant inhibition of ROS formation at 0.01% (p < 0.001), in HaCat, and in a sunscreen at 0.5% (w/v) (p < 0.001), in RHS. In conclusion, in vitro results showed fucoxanthin protective potential to the skin that might contribute to improving the photoprotective potential of sunscreens in vivo.

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“…Alternative testing models may include ex vivo skin models [24,25], full-thickness human skin equivalent models [26,27], human reconstructed epidermis (RHE) [28,29] and human immortalised keratinocytes (HaCaTs). HaCaTs is used widely as an in vitro monolayer models for cosmetic safety testing [30,31].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Evaluation of Hair Relaxers and Straighteners Actives in Human-Derived Skin Cells

Lebeko¹,

Kidzeru²,

Sishi³

et al. 2021

Preprint

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Most hair relaxers or straighteners used for Afro-textured hair have a pH above 11. This pH, according to international occupational health and safety guidelines, is corrosive to the skin. Further, relaxers are associated with a 3-6 times increased risk of alopecia. The skin also gets into contact with these hair straightening products during application resulting in skin irritation. Using animals as test subjects to determine these products' potential risk and safety margin is standard practice; however, with the European Union (EU) ban on animal testing, the testing method needs to be replaced by newer in vitro laboratory technologies. This study used previously and newly established in vitro cell-based technologies and human-skin derived cells as laboratory models to evaluate relaxer-induced cytotoxicity. These technologies include endpoint (MTT, CCK-8 and flow cytometry) and real-time assays (real-time cell analysis (RTCA) and extracellular flux analysis). Cellular toxicity was evident following treatment of keratinocytes and fibroblasts with acceptable concentrations of thioglycolic acid (TGA, HSCH2COOH), ammonium bisulphite (NH4HSO3), lithium hydroxide (LiOH) and sodium hydroxide (NaOH) based relaxers. Real-time assays showed significant reductions (P<0.001) in cell index and ATP production following treatment of keratinocytes with as little as one-tenth of the acceptable concentrations. Increased apoptosis (47.9%, 58.0%, 76.7% and 80.3%) also occurred in cells after treatment with TGA, NH4HSO3, NaOH and LiOH, respectively. In conclusion, this study demonstrated that using skin cells in conjunction with advanced cell-based technologies could serve as alternatives to laboratory animals for accessing the toxicity margin of hair relaxers, straighteners and other cosmetics.

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Skin corrosion test: a comparison between reconstructed human epidermis and full thickness skin models

Cited by 28 publications

References 29 publications

New Sustainable Process for Hesperidin Isolation and Anti-Ageing Effects of Hesperidin Nanocrystals

New Sustainable Process for Hesperidin Isolation and Anti-Ageing Effects of Hesperidin Nanocrystals

Fucoxanthin for Topical Administration, a Phototoxic vs. Photoprotective Potential in a Tiered Strategy Assessed by In Vitro Methods

Cytotoxic Evaluation of Hair Relaxers and Straighteners Actives in Human-Derived Skin Cells

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