New Sustainable Process for Hesperidin Isolation and Anti-Ageing Effects of Hesperidin Nanocrystals

Abstract

Hesperidin, a secondary orange (Citrus sinensis) metabolite, was extracted from orange bagasse. No organic solvents or additional energy consumption were used in the clean and sustainable process. Hesperidin purity was approximately 98% and had a yield of 1%. Hesperidin is a known supplement due to antioxidant, chelating, and anti-ageing properties. Herein, hesperidin application to eliminate dark eye circles, which are sensitive and thin skin regions, was studied. In addition, the proposed method for its aqueous extraction was especially important for human consumption. Further, the most effective methods for hesperidin nanonization were explored, after which the nanoemulsions were incorporated into a cream formulation that was formulated for a tropical climate. Silky cream formulations (oil in water) were tested in vitro on artificial 3D skin from cultured cells extracted from skin residues after plastic surgery. The proposed in vitro assay avoided tests of the different formulations in human volunteers and animals. It was shown that one of the nanonized hesperidin formulations was the most skin-friendly and might be used in cosmetics.

Optimizing Procedures of Ultrasound-Assisted Extraction of Waste Orange Peels by Response Surface Methodology

“…No considerable differences between the sample with treatment 14 (sample extracted by 100% methanol with 55 min at 25 °C using 40 kHz) and 15 (sample extracted by 75% ethanol with 85 min at 25 °C using 28 kHz) were observed in accordance with their spectra in the infrared range. Further investigation displays that extract from orange peels ( Figure 3 a) possessed a similar fingerprint of hesperidin ( Figure 3 b) in the wavelength from 500 cm −1 to 4000 cm − 1 [ 41 ], indicating that it contains hesperidin.…”

“…It was stated that the vibration stretching for -C=O in hesperidin was at 1644 cm −1 and the absorption peaks for C-O were detected at 1297, 1275, 1242, 1206, 1184, 1154, 1132, 1095, 1054, 1037, and 1009 cm −1 [ 41 ]. The absorption peaks detected at 3544 and 2924 cm −1 ( Figure 3 ) may be related to hydroxyl (-OH) [ 42 ] and aliphatic (CH) [ 43 ] stretching vibration for hesperidin.…”

“… The transmittance of precipitation with different ultrasonic treatments mixed with potassium bromide detected by FTIR ( a ) and hesperidin–copper complex cited from the study of Stanisic et al (2020) [ 41 ] ( b ). …”

Nanoparticle platforms for dermal antiaging technologies: Insights in cellular and molecular mechanisms

“…This formulation drastically prevented UV-associated mitochondrial depolarization in human dermal fibroblasts (Bruge et al, 2013). Nanocrystals of hesperidin (150-200 nm) interact at molecular level with MMPs as partially immobilized and chelates the metallic ion responsible for activity of MMPs (Stanisic et al, 2020).…”

Editorial to the Special Issue “Biological and Pharmacological Activity of Plant Natural Compounds”

“…Hesperidin (HSP), one of the principal bioflavonoids of Citrus fruits, was extracted from orange bagasse and tested to eliminate dark eye circles in in vitro artificial 3D skin [ 15 ]. The most effective methods for HSP nanonization were explored, and this nanonized compound was found to be the most skin-friendly and could be potentially used in cosmetics.…”