Skin cells undergo asynthetic fission to expand body surfaces in zebrafish

Chan, Keat Ying; Yan, Ching‐Cher Sanders; Roan, Hsiao-Yuh; Hsu, Shao-Chun; Tseng, Tzu-Lun; Hsiao, Cheng‐Chieh; Hsu, Chao‐Ping; Chen, Chen‐Hui

doi:10.1038/s41586-022-04641-0

Cited by 26 publications

(15 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The outer cell layer, periderm, consists of keratinocytes which show characteristic actin ridges and are tightly connected, the underlying layer consists of basal cells which in adult zebrafish harbor a stem cell pool to replace dying cell in the skin (Lee, Asharani and Carney, 2014). During larval development the periderm is gradually replaced and until then grows by divisions and asymmetric fission (Chan et al ., 2022). A functional inflammasome can be induced in both layers of the larval zebrafish skin by over-expressing Asc-mKate2, leading to immediate cell death of periderm and basal cells, (Kuri et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

The Opto-inflammasome in zebrafish: a tool to study cell and tissue responses to speck formation and cell death

Carvalho¹,

Dharmadhikari²,

Leptin³

et al. 2022

Preprint

View full text Add to dashboard Cite

The inflammasome is a conserved system for the intracellular detection of danger or pathogen signals. By forming a large intracellular multiprotein signaling platform, it activates downstream effectors that initiate a rapid necrotic programmed cell death (PCD) termed pyroptosis and activation and secretion of pro-inflammatory cytokines to warn and activate surrounding cells. However, inflammasome activation is difficult to control on a single-cell level using canonical triggers. We constructed Opto-Asc, a light-responsive form of the inflammasome adaptor protein ASC (Apoptosis-Associated Speck-Like Protein Containing a CARD) which allows tight control of inflammasome formation in vivo. We introduced a cassette of this construct under the control of a heat shock element into zebrafish in which we can now induce Asc inflammasome (speck) formation in single cells of the skin. We find that cell death resulting from Asc speck formation is morphological distinct from apoptosis in periderm but not in basal cells. Asc induced PCD in can lead to apical or basal extrusion from the periderm. The apical extrusion in periderm cells depends on Caspb but and triggers a strong Ca2+ signaling response in nearby cells.

show abstract

Section: Introductionmentioning

confidence: 99%

The Opto-inflammasome in zebrafish: a tool to study cell and tissue responses to speck formation and cell death

Carvalho¹,

Dharmadhikari²,

Leptin³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The early embryos of zebrafish undergo rapid cellular cleavage without cell cycle checkpoints until the MBT 39 , and studies have shown the existence of asynthetic fission in early embryos, where cells divide with minimal or no DNA synthesis 16,17,40 . In this study, we found that the deletion of mcm6l and npm2a can affect DNA replication or nuclear division, leading to many anucleated cells.…”

Section: Discussionmentioning

confidence: 99%

Screening of functional maternal-specific chromatin regulators in early embryonic development

Liu,

Wang,

Wang

et al. 2024

Preprint

View full text Add to dashboard Cite

The early stages of embryonic development rely on maternal products for proper regulation. However, systematic screening for functional maternal-specific factors has been challenging due to the time- and labor-intensive nature of traditional approaches. Here, we combined a computational pipeline and F0 homozygous mutation technology to screen for functional maternal-specific chromatin regulators in zebrafish embryogenesis and identified Mcm3l, Mcm6l, and Npm2a as playing essential roles in DNA replication and cell division. Our results contribute to understanding the molecular mechanisms underlying early embryo development and highlight the importance of maternal-specific chromatin regulators in this critical stage.

show abstract

“…The dividing of a cell without DNA replication into functionally equivalent but genetically distinct daughters (meiosis notwithstanding) is not unprecedented in animals. The superficial epithelial cells of the larval zebrafish skin were recently discovered to divide after terminal differentiation and produce hypoploid descendants 97 . Unlike DTC fragments, however, these descendants each have reduced nuclear content but none lack a nucleus entirely.…”

Section: Discussionmentioning

confidence: 99%

Getting there in one piece: The Rac pathway prevents cell fragmentation in a nonprotrusively migrating leader cell during organogenesis

Singh,

Li,

Gordon

2023

Preprint

View full text Add to dashboard Cite

SUMMARYTheC. eleganshermaphrodite distal tip cell (DTC) leads gonadogenesis. Loss-of-function mutations in aC. elegansortholog of the Rac1 GTPase (ced-10) and its GEF complex (ced-5/DOCK180,ced-2/CrkII,ced-12/ELMO) cause gonad migration defects related to directional sensing; we discovered an additional defect class of gonad bifurcation in these mutants. Using genetic approaches, tissue-specific and whole-body RNAi, andin vivoimaging of endogenously tagged proteins and marked cells, we find that loss of Rac1 or its regulators causes the DTC to fragment as it migrates. Both products of fragmentation—the now-smaller DTC and the membranous patch of cellular material—localize important stem cell niche signaling (LAG-2/DSL ligand) and migration (INA-1/integrin subunit alpha) factors to their membranes, but only one retains the DTC nucleus and therefore the ability to maintain gene expression over time. The enucleate patch can lead a bifurcating branch off the gonad arm that grows through germ cell proliferation. Germ cells in this branch differentiate as the patch loses LAG-2 expression. While the nucleus is surprisingly dispensable for aspects of leader cell function, it is required for stem cell niche activity long-term. Prior work found thatRac1−/−;Rac2−/−mouse erythrocytes fragment; in this context, our new findings support the conclusion that maintaining a cohesive but deformable cell is a conserved function of this important cytoskeletal regulator.

show abstract

Skin cells undergo asynthetic fission to expand body surfaces in zebrafish

Cited by 26 publications

References 62 publications

The Opto-inflammasome in zebrafish: a tool to study cell and tissue responses to speck formation and cell death

The Opto-inflammasome in zebrafish: a tool to study cell and tissue responses to speck formation and cell death

Screening of functional maternal-specific chromatin regulators in early embryonic development

Getting there in one piece: The Rac pathway prevents cell fragmentation in a nonprotrusively migrating leader cell during organogenesis

Contact Info

Product

Resources

About