Skin Barrier Recovery by Protease-Activated Receptor-2 Antagonist Lobaric Acid

Joo, Yeon Ah; Chung, Hyunjin; Yoon, Sohyun; Park, Jong Il; Lee, Ji Eun; Myung, Cheol Hwan; Hwang, Jae Sung

doi:10.4062/biomolther.2016.011

Cited by 14 publications

(6 citation statements)

References 27 publications

(33 reference statements)

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“…Another study found that the corneal epithelial barrier function could be altered by PAR2 activation [Li et al, ]. The skin barrier function could be compromised by PAR2 activation [Joo et al, ]. Our data are in line with these pioneer studies by showing that activation of PAR2 compromise epithelial barrier integrity.…”

Section: Discussionsupporting

confidence: 91%

Proteinase‐Activated Receptor‐2 Modulates Ve‐Cadherin Expression to Affect Human Vascular Endothelial Barrier Function

Zhang

2017

J of Cellular Biochemistry

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Published data indicate that the protease-activated receptor (PAR) 2 is involved in the pathogenesis of some cardiovascular diseases; the underlying mechanism is to be further investigated. Ve-cadherin is a critical molecule in maintaining the endothelial barrier integrity. This study aims to investigate the role of PAR2 activation in compromising the cardiac endothelial barrier function. In this study, human umbilical vein endothelial cells (Huvec cells) were cultured into monolayers using as an in vitro model of barrier function. The transepithelial electric resistance (TER) and permeability to dextran were assessed as indicators of barrier function. The expression of Ve-cadherin in Huvec cells was assessed by real-time RT-PCR, Western blotting, and chromatin immunoprecipitation. The results showed that exposure to tryptase in the culture, the barrier function of the Huvec monolayers, was markedly compromised; the levels of Ve-cadherin, one of the tight junction proteins, were suppressed as well. This was mimicked by exposing Huvec monolayers to the active PAR2 peptides (PAR2AP). After exposing to PAR2AP, the levels of histone deacetylase (HDAC)11 were increased in the Huvec cells. HDAC11 formed a complex with the transcription factor of Ve-cadherin to attenuate the Erg gene transcription activities and suppressed the expression of Ve-cadherin. In conclusion, activation of PAR2 compromises the vascular endothelial barrier function by suppressing the expression of Ve-cadherin. J. Cell. Biochem. 118: 4587-4593, 2017. © 2017 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 91%

Proteinase‐Activated Receptor‐2 Modulates Ve‐Cadherin Expression to Affect Human Vascular Endothelial Barrier Function

Zhang

2017

J of Cellular Biochemistry

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“…IVL is synthesized in the spinal cell layer and cross-linked with granulosa cells under the action of glutamine transferase to form an important structural support for the skin barrier (45). At present, IVL is mostly used as a marker protein to study skin keratinocyte differentiation (46,47). However, there are few reports of the associations between integrin and melanoma (48,49).…”

Section: Discussionmentioning

confidence: 99%

Bioinformatic Analysis Identifies Potential Key Genes in the Pathogenesis of Melanoma

Han¹,

Li²,

Yan³

et al. 2020

Front. Oncol.

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Melanoma is the deadliest skin tumor and is prone to distant metastases. The incidence of melanoma has increased rapidly in the past few decades, and current trends indicate that this growth is continuing. This study was aimed to explore the molecular mechanisms of melanoma pathogenesis and discover underlying pathways and genes associated with melanoma. We used high-throughput expression data to study differential expression profiles of related genes in melanoma. The differentially expressed genes (DEGs) of melanoma in GSE15605, GSE46517, GSE7553, and the Cancer Genome Atlas (TCGA) datasets were analyzed. Differentially expressed genes (DEGs) were identified by paired t-test. Then the DEGs were performed cluster and principal component analyses and protein-protein interaction (PPI) network construction. After that, we analyzed the differential genes through bioinformatics and got hub genes. Finally, the expression of hub genes was confirmed in the TCGA databases and collected patient tissue samples. Total 144 up-regulated DEGs and 16 down-regulated DEGs were identified. A total of 17 gene ontology analysis (GO) terms and 11 pathways were closely related to melanoma. Pathway of pathways in cancer was enriched in 8 DEGs, such as junction plakoglobin (JUP) and epidermal growth factor receptor (EGFR). In the PPI networks, 9 hub genes were obtained, such as loricrin (LOR), filaggrin (FLG), keratin 5 (KRT5), corneodesmosin (CDSN), desmoglein 1 (DSG1), desmoglein 3 (DSG3), keratin 1 (KRT1), involucrin (IVL), and EGFR. The pathway of pathways in cancer and its enriched DEGs may play important roles in the process of melanoma. The hub genes of DEGs may become promising melanoma candidate genes. Five key genes FLG, DSG1, DSG3, IVL, and EGFR were identified in the TCGA database and melanoma tissues. The results suggested that FLG, DSG1, DSG3, IVL, and EGFR might play important roles and potentially be valuable in the prognosis and treatment of melanoma. These hub genes might well have clinical significance as diagnostic markers.

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“…IVL is synthesized in the spinal cell layer and cross-linked with granulosa cells under the action of glutamine transferase to form an important structural support for the skin barrier [42]. At present, IVL is mostly used as a marker protein to study skin keratinocyte differentiation [43,44], however, there are few reports of the associations between integrin and melanoma. In view of its important role in maintaining skin function, we can speculate that IVL may also play an important role in the progression of melanoma.…”

Section: Discussionmentioning

confidence: 99%

A Comprehensive Data Analysis of Differentially Regulated Genes in Melanoma

Han¹,

Li²,

Yan³

et al. 2020

Preprint

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Background: Melanoma is the most deadly tumor in skin tumors and is prone to distant metastases. The incidence of melanoma has increased rapidly in the past few decades, and current trends indicate that this growth is continuing. This study was aimed to explore the molecular mechanisms of melanoma pathogenesis and discover underlying pathways and genes associated with melanoma.Methods: We used high-throughput expression data to study differential expression profiles of related genes in melanoma. The differentially expressed genes (DEGs) of melanoma in GSE15605, GSE46517, GSE7553 and the Cancer Genome Atlas (TCGA) datasets were analyzed. Differentially expressed genes (DEGs) were identified by paired t-test. Then the DEGs were performed cluster and principal component analyses and protein–protein interaction (PPI) network construction. After that, we analyzed the differential genes through bioinformatics and got hub genes. Finally, the expression of hub genes was confirmed in the TCGA databases and collected patient tissue samples.Results: Total 144 up-regulated DEGs and 16 down-regulated DEGs were identified. A total of 17 gene ontology analysis (GO) terms and 11 pathways were closely related to melanoma. Pathway of pathways in cancer was enriched in 8 DEGs, such as junction plakoglobin (JUP) and epidermal growth factor receptor (EGFR). In the PPI networks, 9 hub genes were obtained, such as loricrin (LOR), filaggrin (FLG), keratin 5 (KRT5), corneodesmosin (CDSN), desmoglein 1 (DSG1), desmoglein 3 (DSG3), keratin 1 (KRT1), involucrin (IVL) and EGFR. The pathway of pathways in cancer and its enriched DEGs may play important roles in the process of melanoma. The hub genes of DEGs may become promising melanoma candidate genes. Five key genes FLG, DSG1, DSG3, IVL and EGFR were identified in the TCGA database and melanoma tissues.Conclusions: The results suggested that FLG, DSG1, DSG3, IVL and EGFR might play important roles and potentially be valuable in the prognosis and treatment of melanoma.

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Skin Barrier Recovery by Protease-Activated Receptor-2 Antagonist Lobaric Acid

Cited by 14 publications

References 27 publications

Proteinase‐Activated Receptor‐2 Modulates Ve‐Cadherin Expression to Affect Human Vascular Endothelial Barrier Function

Proteinase‐Activated Receptor‐2 Modulates Ve‐Cadherin Expression to Affect Human Vascular Endothelial Barrier Function

Bioinformatic Analysis Identifies Potential Key Genes in the Pathogenesis of Melanoma

A Comprehensive Data Analysis of Differentially Regulated Genes in Melanoma

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