Skin and subcutaneous mycoses in tilapia (Oreochromis niloticus) caused by Fusarium oxysporum in coinfection with Aeromonas hydrophila

Cutuli, M. Teresa; Gibello, Alicia; Rodríguez‐Bertos, Antonio; Blanco, M. Mar; Villarroel, Morris; Giraldo, Alejandra; Guarro, Josep

doi:10.1016/j.mmcr.2015.06.002

Cited by 46 publications

(36 citation statements)

References 18 publications

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“…Furthermore, experimental challenge of F. oxysporum into kuruma prawn ( Penaeus japonicus ) and Nile tilapia ( O. niloticus ) has been successfully developed into infection and confirmed the pathogenicity of F. oxysporum on aquatic animals (Khoa & Hatai ; Cutuli et al . ). Development of the infection during a pathogenicity test on zebrafish and histopathologic findings indicate the virulence of the fungi and potential risk of spreading among other healthy fish communities.…”

Section: Discussionmentioning

confidence: 97%

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First report of Fusarium oxysporum species complex infection in zebrafish culturing system

Kulatunga

Dananjaya

Park

et al. 2016

Journal of Fish Diseases

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Fusarium oxysporum species complex (FOSC) is a highly diverse fungus. Recently, F. oxysporum infection was identified from zebrafish (Danio rerio) culturing system in Korea. Initially, a rapid whitish smudge was appeared in the water with the fungal blooming on walls of fish tanks. Microscopic studies were conducted on fungal hyphae, colony pigmentation and chlamydospore formation and the presence of macro- and microspores confirmed that the isolated fungus as F. oxysporum. Furthermore, isolated F. oxysporum was confirmed by internal transcribed spacer sequencing which matched (100%) to nine F. oxysporum sequences available in GenBank. Experimental hypodermic injection of F. oxysporum into adult zebrafish showed the development of fungal mycelium and pathogenicity similar to signs observed. Histopathologic results revealed a presence of F. oxysporum hyphae in zebrafish muscle. Fusarium oxysporum growth was increased with sea salt in a concentration-dependent manner. Antifungal susceptibility results revealed that F. oxysporum is resistant to copper sulphate (up to 200 μg mL ) and sensitive to nystatin (up to 40 μg mL ). This is the first report of FOSC from zebrafish culture system, suggesting it appears as an emerging pathogen, thus posing a significant risk on zebrafish facilities in the world.

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Section: Discussionmentioning

confidence: 97%

“…Very recently, the first F. oxysporum infection in freshwater habitat has been reported from cultured Nile tilapia ( Oreochromis niloticus ) in co‐infection with Aeromonas hydrophila (Cutuli et al . ). However, virulence and pathogenicity of FOSC as well as associated environment factors have not investigated in detail.…”

Section: Introductionmentioning

confidence: 97%

First report of Fusarium oxysporum species complex infection in zebrafish culturing system

Kulatunga

Dananjaya

Park

et al. 2016

Journal of Fish Diseases

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“…); or miscellaneous disease caused by multiple infections (Cutuli et al . ; Dong et al . ; Assis et al .…”

Section: Introductionmentioning

confidence: 99%

Aeromonas jandaei and Aeromonas veronii caused disease and mortality in Nile tilapia, Oreochromis niloticus (L.)

Dong

Techatanakitarnan

Jindakittikul

et al. 2017

Journal of Fish Diseases

195

156

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Diseases caused by motile aeromonads in freshwater fish have been generally assumed to be linked with mainly Aeromonas hydrophila while other species were probably overlooked. Here, we identified two isolates of non-A. hydrophila recovered from Nile tilapia exhibiting disease and mortality after exposed to transport-induced stress and subsequently confirmed their virulence in artificial infection. The bacterial isolates were identified as Aeromonas jandaei and Aeromonas veronii based on phenotypic features and homology of 16S rDNA. Experimental infection revealed that the high dose of A. jandaei (3.7 × 10 CFU fish ) and A. veronii (8.9 × 10 CFU fish ) killed 100% of experimental fish within 24 h, while a 10-fold reduction dose killed 70% and 50% of fish, respectively. When the challenge dose was reduced 100-fold, mortality of the fish exposed to A. jandaei and A. veronii decreased to 20% and 10%, respectively. The survivors from the latter dose administration were rechallenged with respective bacterial species. Lower mortality of rechallenged fish (0%-12.5%) compared to the control groups receiving a primary infection (37.5%) suggested that the survivors after primary infection were able to resist secondary infection. Fish exposed to either A. jandaei or A. veronii exhibited similar clinical signs and histological manifestation.

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“…They are known to be opportunistic microorganisms which may cause local or systemic infections and present the most diverse antifungal resistance panel of drugs that include voriconazole, posaconazole, and amphotericin B. Clinical manifestation depends on the immune status of the host being neutropenia the major risk factor for acquiring infections . Fusariosis represents the second most prevalent cause of systemic filamentous fungal infection after aspergillosis .…”

Section: Introductionmentioning

confidence: 99%

“…Other species have a lower incidence of infections . Invasive fusariosis usually attacks immunocompromised patients while immunocompetent individuals usually are affected by local infections ,,. With regard to pathogenicity, F. solani represents the most virulent genus of the species .…”

Section: Introductionmentioning

confidence: 99%

Allylic Selenocyanates as New Agents to Combat Fusarium Species Involved with Human Infections

et al. 2017

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Fusarium is an emerging opportunistic fungal pathogen that causes local or systemic infections. The successful use of a therapeutic drug or combination antifungal therapies against Fusarium spp. are compromised because reports of multidrug resistance are currently frequent. Thus, the development of new antifungal capable of combating multidrug‐resistant Fusarium strains becomes necessary. This study presents the synthesis of seven new allylic selenocyanates and their screening against Fusarium spp. Minimum inhibitory concentrations (MICs) of these compounds ranged from 4 to 64 μg mL−1, with the mechanism of action being related to fungal cell membrane disruption. Specific structural changes, such as widespread thinning along the hyphae, were observed by scanning electron microscopy. The effect of selenocyanates on cell viability and genotoxicity are concentration dependent, however they did not cause mutagenicity in human cells. Five selenocyanates were identified as nonirritant by the ex‐vivo HET‐CAM (Hen′s Egg Test‐Chorioallantoic Membrane) method. Allylic selenocyanates represents a promising alternative in the treatment and prevention of fusariosis.

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Skin and subcutaneous mycoses in tilapia (Oreochromis niloticus) caused by Fusarium oxysporum in coinfection with Aeromonas hydrophila

Cited by 46 publications

References 18 publications

First report of Fusarium oxysporum species complex infection in zebrafish culturing system

First report of Fusarium oxysporum species complex infection in zebrafish culturing system

Aeromonas jandaei and Aeromonas veronii caused disease and mortality in Nile tilapia, Oreochromis niloticus (L.)

Allylic Selenocyanates as New Agents to Combat Fusarium Species Involved with Human Infections

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