Skeletal alterations in ovariectomized rats

Wronski, Thomas J.; Lowry, P. L.; Walsh, C. C.; Ignaszewski, L. A.

doi:10.1007/bf02554882

Cited by 376 publications

(193 citation statements)

References 24 publications

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“…However, in the GX group, but not in the OVX group, cortical BMC was reduced as well. These findings accord with those of others (Wronski et al 1985, Surve et al 2001a. OVX was associated with an increase in body weight.…”

Section: Discussionsupporting

confidence: 93%

Pharmacological treatment of osteopenia induced by gastrectomy or ovariectomy in young female rats

Andersson

Surve

Lehto-Axtelius

et al. 2004

Acta Orthopaedica Scandinavica

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Background Both gastrectomy (GX) and ovariectomy (OVX) induce osteopenia in man and experimental animals. The present study addresses the question -can alendronate, estrogen or parathyroid hormone (PTH) be used to treat established GX-or OVX -evoked osteopenia?Methods Rats were GX-, OVX-or SHAM-operated 8 weeks before starting the treatment with drugs. Each group was then treated for 8 weeks with 50 µg/kg/day alendronate, 10 µg/kg/day estrogen or 75 µg/kg/day PTH(1-84); n = 8 rats/group. Peripheral Quantitative Computed Tomography (pQCT) was used to measure trabecular bone mineral density (BMD) and various cortical bone parameters.Results At killing, 16 weeks after surgery, GX and OVX rats had a greatly reduced trabecular BMD in the metaphysis of the distal femur (GX -44% and OVX -55%). Alendronate increased the trabecular BMD by 44% in GX rats and by 64% in OVX rats, while PTH increased it by 51% and 115%, respectively. However, estrogen increased the trabecular BMD in GX rats (35%), but not in OVX rats (15%, not significant). Cortical bone parameters were adversely (but moderately) affected by GX, but not by OVX or by treatment with the three drugs.Interpretation Alendronate, estrogen and PTH restored the trabecular bone loss in rats with an established GX-evoked osteopenia. In contrast, alendronate and PTH, but not estrogen, restored the trabecular bone loss after OVX. Hence, the mechanism underlying GX-evoked bone loss differs from that underlying OVXevoked bone loss. The ability of alendronate, estrogen

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Section: Discussionsupporting

confidence: 93%

Pharmacological treatment of osteopenia induced by gastrectomy or ovariectomy in young female rats

Andersson

Surve

Lehto-Axtelius

et al. 2004

Acta Orthopaedica Scandinavica

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“…There was no significant difference among the groups, a fact that contrasts with the literature data that demonstrated higher weight gains in the oophorectomized animals 10 . This is a fact that minimizes bone loss but does not prevent it 11 .…”

Section: Discussioncontrasting

confidence: 99%

Pamidronate and zoledronate effects in the increment of bone mineral density and histomorphometry in rats

Júnior¹,

Buchpiguel

Guarniero³

et al. 2011

Acta Cir. Bras.

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Purpose:To compare increment of bone mineral density (BMD) with pamidronate, zoledronate and the isolated effect of proteinous diet in undernourished oophorectomized and non-oophorectomized female rats, besides validating BMD's indexes. Methods: 60 young female Lewis rats were divided into five experimental groups and a control group, oophorectomized and non-oophorectomized. The administration of drugs were submitted to proteinous and aproteinous diets. The variables analyzed were weight, bone densitometry, histomorphometry and biochemical evolution. Results: In weight evaluation, the first interval showed a statistically meaningful increase in oophorectomized sample. In densitometry evaluation, the first interval showed statistically meaningful decrease in four medicated groups and third showed a statistically meaningful increase in 2 non-oophorectomized groups. In laboratory evaluation, there were an increase of total proteins and globulin, decrease of alkaline phosphatase, phosphorus and calcium (except for the oophorectomized) in four medicated groups and increase of phosphorus and calcium in 2 not medicated groups. In histomorphometric evaluation, the oophorectomized groups had smaller increment of BMD. Conclusions: The pamidronate and zoledronate have shown effectives in the increment of BMD. The proteinous diet itself possesses therapeutic effect in BMD though not significant compared with medicated animals. The results of histomorphometry allow validating BMD's indexes in this experimental model. Key words: Osteoporosis. Bone Density. Densitometry. Ovariectomy, Diphosphonates. Rats. RESUMO Objetivo:Comparar o incremento de densidade mineral óssea (DMA) com pamidronato, zoledronato e o efeito isolado de dieta protéica em ratas desnutridas ooforectomizadas e não ooforectomizadas, além de validar índices de DMA. Métodos: 60 ratas jovens Lewis foram divididas em cinco grupos experimentais e um grupo controle, com e sem ooforectomia. A administração das drogas foi submetida às dietas protéica e aprotéica. As variáveis analisadas foram peso, densitometria óssea, histomorfometria e evolução bioquímica. Resultados: Na avaliação ponderal, o primeiro intervalo mostrou aumento estatisticamente significante nos grupos ooforectomizados. Na avaliação densitométrica, o primeiro intervalo mostrou diminuição estatisticamente significante nos quatro grupos medicados e o terceiro mostrou aumento estatisticamente significante nos dois não ooforectomizados. Na avaliação laboratorial, houve aumento de proteínas totais e globulina, diminuição de fosfatase alcalina, fósforo e cálcio (exceto nos ooforectomizados) nos quatro grupos medicados e aumento de fósforo e cálcio nos dois não medicados. Na avaliação histomorfométrica, os grupos ooforectomizados tiveram incremento menor de DMA. Conclusões: O pamidronato e o zoledronato se mostraram efetivos no incremento de DMA. A dieta protéica em si possui efeito terapêutico na DMA, porém menos significativa, comparada aos animais medicados. Os resultados da histomorfometria per...

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“…In brief, it has first been reported that, at 5 weeks post-OVX performed on rats of approximately 75 days of age, all histomorphometric indices of bone formation rate in OVX animals were increased in comparison with normal rats [40]. Later, the same group has reported that, at different times from 14 to 180 days post-OVX performed on 90-day old rats, some, but not all, indices of bone formation rate already considered in the previous work differed between OVX and normal rats [39].…”

Section: Discussionmentioning

confidence: 99%

“…However, these conclusions are clouded with issues of correctness affecting the analysis of the data. First, the high standard deviations in the dynamic parameters of bone formation, as noted by the authors themselves [40], jeopardize the conclusion of post-OVX increases in bone formation rates. Second, it is not immediately clear how a truly two-factorial design and a clear interaction between time and animal status, which is obvious from the depicted data, was tackled by using a two-tailed Student´s t-test for group comparisons at different time points [39].…”

Section: Discussionmentioning

confidence: 99%

Changes in bone Pb accumulation: Cause and effect of altered bone turnover

Brito

Costa

Silva

et al. 2014

Bone

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This paper assesses the magnitude of Pb uptake in cortical and trabecular bones in healthy animals and animals with altered balance in bone turnover, and the impact of exposure to Pb on serum markers of bone formation and resorption. The results reported herein provide physiological evidence that Pb distributes differently in central compartments in Pb metabolism, such as cortical and trabecular bone, in healthy animals and animals with altered balance in bone turnover, and that exposure to Pb does have an impact on bone resorption resulting in OC-dependent osteopenia. These findings show that Pb may play a role in the etiology of osteoporosis and that its concentration in bones varies as a result of altered bone turnover characteristic of this disease, a long standing question in the field. In addition, data collected in this study are consistent with previous observations of increased half-life of Pb in bone at higher exposures. This evidence is relevant for the necessary revision of current physiologically based kinetic models for Pb in humans. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 Abstract This paper assesses the magnitude of Pb uptake in cortical and trabecular bones in healthy animals and animals with altered balance in bone turnover, and the impact of exposure to Pb on serum markers of bone formation and resorption. The results reported herein provide physiological evidence that Pb distributes differently in central compartments in Pb metabolism, such as cortical and trabecular bone, in healthy animals and animals with altered balance in bone turnover, and that exposure to Pb does have an impact on bone resorption resulting in OC-dependent osteopenia. These findings show that Pb may play a role in the etiology of osteoporosis and that its concentration in bones varies as a result of altered bone turnover characteristic of this disease, a long standing question in the field. In addition, data collected in this study are consistent with previous observations of increased half-life of Pb in bone at higher exposures. This evidence is relevant for the necessary revision of current physiologically based kinetic models for Pb in humans. Keywords Pb exposureBone turnover Osteoporosis Kinetic models 1

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Skeletal alterations in ovariectomized rats

Cited by 376 publications

References 24 publications

Pharmacological treatment of osteopenia induced by gastrectomy or ovariectomy in young female rats

Pharmacological treatment of osteopenia induced by gastrectomy or ovariectomy in young female rats

Pamidronate and zoledronate effects in the increment of bone mineral density and histomorphometry in rats

Changes in bone Pb accumulation: Cause and effect of altered bone turnover

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