Sizing of the Leptospira genome by pulsed-field agarose gel electrophoresis

Baril, C; Girons, Isabelle Saint

doi:10.1111/j.1574-6968.1990.tb03804.x

Cited by 27 publications

(8 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taylor et al suggested that the L. interrogans chromosome is linear, fragmented, and approximately 3.1 Mbp (36). However, we found it to be circular and 4.6 Mbp, in agreement with our earlier results (2) and the report of Zuerner (41). Such a map will provide a point of reference from which the linkage of specific genes can be established.…”

supporting

confidence: 82%

“…The L. interrogans chromosome size determined by PFGE of NotI, Sifl, and AscI digests was estimated to be approximately 4.6 Mbp, which is close to the size of 5 Mbp obtained previously (2). These results suggest that the chromosome size of L. interrogans is of the same order of magnitude as that of E. coli, which correlates with their ability to grow in a defined medium; other fastidious spirochetes such as Treponema pallidum and Borrelia burgdorferi have chromosomes about one-fifth of this size (1,6,9,38).…”

Section: Discussionmentioning

confidence: 64%

“…In a preliminary investigation by pulsed-field gel electrophoresis (PFGE), we found that the Leptospira genome contained a 5-Mbp circular chromosome (2). PFGE is now a well-established procedure for the analysis of large DNA fragments (31).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae

et al. 1992

View full text Add to dashboard Cite

Leptospira interrogans is a pathogenic bacterium with a low G+C content (34 to 39%). The restriction enzymes NotI, AscI, and SrfI cut the chromosome of L. interrogans serovar icterohaemorrhagiae into 13, 3, and 5 fragments separable by one- and two-dimensional pulsed-field gel electrophoresis (PFGE). The genome is composed of a circular 4.6-Mbp chromosome and a 0.35-Mbp extrachromosomal element. A physical map of the chromosome was constructed for NotI, AscI, and SrfI by using single and double digests, or partial NotI digests obtained at random or by cross-protection of NotI sites by FnuDII methylase, and linking clones. rRNA genes were found to be widely scattered on the chromosome.

show abstract

supporting

confidence: 82%

Section: Discussionmentioning

confidence: 64%

See 1 more Smart Citation

Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae

et al. 1992

View full text Add to dashboard Cite

show abstract

“…Studies on different species of the genus Borrelia (12,29) have shown both the linear character and the size of the chromosomes to be similar, as indeed is the genetic arrangement (26). In the different Leptospira strains studied (4,40), two circular chromosomes of 4.6 Mbp and 350 kbp have been shown to exist. However, differences in physical and genetic makeup are apparent on more detailed analysis and comparison of these chromosomes (40,41), despite the bacteria in question being of the same species.…”

Section: Discussionmentioning

confidence: 99%

Physical map of the Treponema denticola circular chromosome

MacDougall

Girons

1995

J Bacteriol

View full text Add to dashboard Cite

A physical map of the Treponema denticola ATCC 33520 genome was constructed by pulsed-field gel electrophoresis and DNA hybridization. The organism possesses a single, circular chromosome of approximately 3.0 Mbp and a 2.6-kbp circular plasmid, pTD1. The physical map of the A؉T-rich genome was constructed with the rare-cutting restriction enzymes AscI, NotI, and SrfI, which have 8-bp G؉C-rich recognition sites. The genes flgE, tdpA, and prtB encoding the flagellar hook protein, a 53-kDa immunogenic protein, and chymotrypsinlike protease, respectively, were located on the map. This treponeme was found to have two copies of each of the rRNA genes, as has been found to be the case for both Treponema phagedenis and Treponema pallidum.

show abstract

“…The genome size of L. interrogans was estimated at 5,000 kb by using PFGE after restriction with three different endonucleases, and the pattern of each of the three serovars analyzed was different (3).…”

mentioning

confidence: 99%

Genome conservation in isolates of Leptospira interrogans

et al. 1991

View full text Add to dashboard Cite

Reference strains for each of the 23 serogroups of Leptospira interrogans yielded different pulsed-field gel electrophoresis patterns of NotI digestion products. This was also the case for the 14 serovars belonging to serogroup Icterohaemorrhagiae (with one exception). The NotI restriction patterns of 45 clinical leptospiral isolates belonging to serovar icterohaemorrhagiae were analyzed and compared with those of type strains. No differences were observed between isolates from countries of different continents, namely, France, French Guiana, New Caledonia, and Tahiti. The pattern was indistinguishable from that of the reference strain of serovar icterohaemorrhagiae.

show abstract

Sizing of the Leptospira genome by pulsed-field agarose gel electrophoresis

Cited by 27 publications

References 12 publications

Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae

Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae

Physical map of the Treponema denticola circular chromosome

Genome conservation in isolates of Leptospira interrogans

Contact Info

Product

Resources

About