Physical map of the Treponema denticola circular chromosome

MacDougall, Jane; Girons, Isabelle Saint

doi:10.1128/jb.177.7.1805-1811.1995

Cited by 16 publications

(11 citation statements)

References 40 publications

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“…As a positive control, the genome size of T. denticola ATCC 33520 was determined. The result obtained, 2,984 kb, agrees closely with that previously obtained, 3,030 kb (5). The genome sizes for seven species representing six distinct groups of treponemes are surprisingly consistent at 2.2–2.5 Mbp.…”

Section: Sizes Of Restriction Fragments In Kb Of the Genomes Of Varsupporting

confidence: 92%

“…The purpose of this work was to determine the genome sizes of representative strains of the cultivable oral treponeme groups using pulsed‐field gel electrophoresis. This approach has been used to accurately estimate the size of the genome of T. denticola ATCC 33520 prior to its sequencing (5). Such information allows inferences to be made about the complexity of the genome of each Treponema group.…”

Section: Sizes Of Restriction Fragments In Kb Of the Genomes Of Varmentioning

confidence: 99%

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Genome size of human oral Treponema species by pulsed‐field gel electrophoresis

Correia¹,

Plummer²,

Paster³

et al. 2004

Oral Microbiology and Immunology

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The genome sizes of seven strains of oral treponemes were determined using pulsed-field gel electrophoresis (PFGE). These strains represent members from six of the currently known cultivable oral treponeme groups. The PFGE fragments were digitally recorded and then quantitated using GIMP v 1.2, an image manipulation program. The results show that the six oral treponeme genomes are comparable in size, ranging from approximately 2.2 to 2.5 Mbp. The genome sizes of these strains are 20-25% smaller than Treponema denticola strains, which have genome sizes of approximately 2.8-3.0 Mbp.

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Section: Sizes Of Restriction Fragments In Kb Of the Genomes Of Varsupporting

confidence: 92%

Section: Sizes Of Restriction Fragments In Kb Of the Genomes Of Varmentioning

confidence: 99%

Genome size of human oral Treponema species by pulsed‐field gel electrophoresis

Correia¹,

Plummer²,

Paster³

et al. 2004

Oral Microbiology and Immunology

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show abstract

“…It has been proposed that the 1.14 Mb T. pallidum genome was derived from the genome of T. denticola or other host-associated treponemes through deletion and divergence (15). Comparisons reveal that the T. pallidum genome shares limited nucleotide identity with the T. denticola genome.…”

Section: Resultsmentioning

confidence: 99%

Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes

Seshadri

Myers

Tettelin

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

251

314

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We present the complete 2,843,201-bp genome sequence of Treponema denticola (ATCC 35405) an oral spirochete associated with periodontal disease. Analysis of the T. denticola genome reveals factors mediating coaggregation, cell signaling, stress protection, and other competitive and cooperative measures, consistent with its pathogenic nature and lifestyle within the mixed-species environment of subgingival dental plaque. Comparisons with previously sequenced spirochete genomes revealed specific factors contributing to differences and similarities in spirochete physiology as well as pathogenic potential. The T. denticola genome is considerably larger in size than the genome of the related syphiliscausing spirochete Treponema pallidum. The differences in gene content appear to be attributable to a combination of three phenomena: genome reduction, lineage-specific expansions, and horizontal gene transfer. Genes lost due to reductive evolution appear to be largely involved in metabolism and transport, whereas some of the genes that have arisen due to lineage-specific expansions are implicated in various pathogenic interactions, and genes acquired via horizontal gene transfer are largely phagerelated or of unknown function.

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“…Moreover, genome sequencing has revealed considerably larger genomes of oral treponemes (e.g. 2.84 Mb for T. denticola , (MacDougall and Girons, 1995; Seshadri et al, 2004) compared to T. pallidum strains (1.14 Mb; Fraser et al, 1998). In this review, we will focus on the genetic diversity of obligatory pathogenic Treponema pallidum strains and on related uncultivable treponemes.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity in Treponema pallidum: Implications for pathogenesis, evolution and molecular diagnostics of syphilis and yaws

Šmajs

Norris

Weinstock³

2012

Infection, Genetics and Evolution

103

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Pathogenic uncultivable treponemes, similar to syphilis-causing Treponema pallidum subspecies pallidum, include T. pallidum ssp. pertenue, T. pallidum ssp. endemicum and Treponema carateum, which cause yaws, bejel and pinta, respectively. Genetic analyses of these pathogens revealed striking similarity among these bacteria and also a high degree of similarity to the rabbit pathogen, T. paraluiscuniculi, a treponeme not infectious to humans. Genome comparisons between pallidum and non-pallidum treponemes revealed genes with potential involvement in human infectivity, whereas comparisons between pallidum and pertenue treponemes identified genes possibly involved in the high invasivity of syphilis treponemes. Genetic variability within syphilis strains is considered as the basis of syphilis molecular epidemiology with potential to detect more virulent strains, whereas genetic variability within a single strain is related to its ability to elude the immune system of the host. Genome analyses also shed light on treponemal evolution and on chromosomal targets for molecular diagnostics of treponemal infections.

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Physical map of the Treponema denticola circular chromosome

Cited by 16 publications

References 40 publications

Genome size of human oral Treponema species by pulsed‐field gel electrophoresis

Genome size of human oral Treponema species by pulsed‐field gel electrophoresis

Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes

Genetic diversity in Treponema pallidum: Implications for pathogenesis, evolution and molecular diagnostics of syphilis and yaws

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