Size‐selective comparison of fetal calvarial versus adult marrow osteogenic colony‐forming entities

Dutra, Timothy F.; Bernard, G. W.

doi:10.1002/ar.1092390102

Cited by 4 publications

(1 citation statement)

References 37 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a simpler protocol, other studies have reported a method using nylon filters for separating cells by cell size from various tissues, for example, spermatozoa, leukocytes, islets and adult marrow-derived cells [11][12][13][14][15]. Another technique, cell isolation based on fluorescenceactivated cell sorting (FACS) provides a reliable method for separating epidermal stem cells by size.…”

Section: Introductionmentioning

confidence: 99%

Isolation of small-sized human epidermal progenitor/stem cells by Gravity Assisted Cell Sorting (GACS)

Fujimori

Izumi

Feinberg

et al. 2009

Journal of Dermatological Science

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Isolation of small-sized human epidermal progenitor/stem cells by Gravity Assisted Cell Sorting (GACS)

Fujimori

Izumi

Feinberg

et al. 2009

Journal of Dermatological Science

View full text Add to dashboard Cite

Production of progenitor cells from primary human epithelial cell monolayer cultures

Miyazawa

Kuo

Feinberg

2018

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

Primary keratinocytes derived from human epidermis are widely used in tissue engineering and regenerative medicine. An important aspect in clinical applications is the preservation of human skin keratinocyte stem cells. However, it is difficult to expand the number of human skin keratinocyte stem cells, which are undifferentiated and highly proliferative in culture by using standard cell culture methods. It is even more difficult to identify them, since universal specific markers for human skin keratinocyte stem cells have not been identified. In this paper, we show a method to produce a large number of primary progenitor human skin keratinocytes by using our novel culture techniques. Primary human skin keratinocyte monolayers are cultured using twice the volume of medium without serum and lacking essential fatty acids. Once the cells reach 70-80% confluence, they begin to float up into the overlying medium and are called "epithelial pop-up keratinocytes (ePUKs)" allowing the cells to be passaged without the use of trypsin. We analyzed the properties of ePUKs by cell size, cell viability, immunocytofluorescence biomarker staining, and cell cycle phase distribution by fluorescence-activated cell sorting (FACS). Our results showed that these ePUKs appear to be progenitor epithelial cells, which are small in size, undifferentiated, and have a high proliferative capacity. We believe that ePUKs are suitable for use in medical applications requiring a large number of primary human progenitor skin keratinocytes.

show abstract

The Faith of a Coronal Suture Grafted Onto Midline Synostosis Inducing Dura and Deprived From Tensile Stress

Mommaerts

Staels

2001

The Cleft Palate-Craniofacial Journal

View full text Add to dashboard Cite

show abstract

Size‐selective comparison of fetal calvarial versus adult marrow osteogenic colony‐forming entities

Abstract: For adult bone marrow, cell clumps or clusters larger than 105 microns produced bone colonies under standard cell culture conditions, whereas for fetal calvarial cells, the initiating agent was smaller than 30 microns.

Cited by 4 publications

References 37 publications

Isolation of small-sized human epidermal progenitor/stem cells by Gravity Assisted Cell Sorting (GACS)

Isolation of small-sized human epidermal progenitor/stem cells by Gravity Assisted Cell Sorting (GACS)

Production of progenitor cells from primary human epithelial cell monolayer cultures

The Faith of a Coronal Suture Grafted Onto Midline Synostosis Inducing Dura and Deprived From Tensile Stress

Contact Info

Product

Resources

About