Size of silver nanoparticles determines proliferation ability of human circulating lymphocytes in vitro

Joksić, Gordana; Stašić, J.; Filipović, Jelena; Šobot, Ana Valenta; Trtica, M.

doi:10.1016/j.toxlet.2016.02.007

Cited by 17 publications

(16 citation statements)

References 20 publications

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“…Treated cells were characterized by the presence of intracytoplasmic vacuoles, enlarged multi-lobed nuclei, ruffled cell surface, apoptotic bodies and condensed chromatin that are clear sign of mechanical stress and apoptosis ( Figure 3 ). Similar morphological changes of hPBMC treated with AgNPs were reported by Zhornik et al [ 30 ] and Joksić et al [ 31 ].…”

Section: Resultssupporting

confidence: 87%

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Surface Stabilization Affects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells

et al. 2020

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Silver nanoparticles (AgNPs) are one of the most investigated metal-based nanomaterials. Their biocidal activity boosted their application in both diagnostic and therapeutic medical systems. It is therefore crucial to provide sound evidences for human-related safety of AgNPs. This study aimed to enhance scientific knowledge with regard to biomedical safety of AgNPs by investigating how their different surface properties affect human immune system. Methods: preparation, characterization and stability evaluation was performed for four differently coated AgNPs encompassing neutral, positive and negative agents used for their surface stabilization. Safety aspects were evaluated by testing interaction of AgNPs with fresh human peripheral blood mononuclear cells (hPBMC) by means of particle cellular uptake and their ability to trigger cell death, apoptosis and DNA damages through induction of oxidative stress and damages of mitochondrial membrane. Results: all tested AgNPs altered morphology of freshly isolated hPBMC inducing apoptosis and cell death in a dose- and time-dependent manner. Highest toxicity was observed for positively-charged and protein-coated AgNPs. Cellular uptake of AgNPs was also dose-dependently increased and highest for positively charged AgNPs. Intracellularly, AgNPs induced production of reactive oxygen species (ROS) and damaged mitochondrial membrane. Depending on the dose, all AgNPs exhibited genotoxic potential. Conclusions: this study provides systematic and comprehensive data showing how differently functionalized AgNPs may affect the human immune system. Presented results are a valuable scientific contribution to safety assessment of nanosilver-based blood-contacting medical products.

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Section: Resultssupporting

confidence: 87%

“…Significant increase of TL indicated that fragmentation of the DNA started, but only the TI parameter reliably indicated the percentage of damaged DNA in tails. Similar genotoxic effects were also evidenced for hPBMC treated by small sized non-coated Ag nanoclusters [34] and non-coated AgNPs [31].…”

Section: Genotoxicity Of Agnpssupporting

confidence: 72%

Surface Stabilization Affects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells

et al. 2020

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“…In general, smaller NPs are more toxic than larger ones [14]. Several works have confirmed this relationship and some authors have identified NP sizes that correlate well with the level of toxicity observed on in vitro tests [15].…”

Section: Physicochemical Characteristics Of Nanomaterials and Their Imentioning

confidence: 76%

Cytotoxic and Antiproliferative Effects of Nanomaterials on Cancer Cell Lines: A Review

Grijalva¹,

Vallejo-López²,

Salazar³

et al. 2018

Unraveling the Safety Profile of Nanoscale Particles and Materials - From Biomedical to Environmental Applications

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Cell models for the study of antiproliferative and/or cytotoxic properties of engineered nanoparticles are valuable tools in cancer research. Several techniques and methods are readily available for the study of nanoparticles' properties regarding selective toxicity and/or antiproliferative effects. Setting up of those techniques, however, needs to be carefully monitored. Harmonization of the wide range of methods available is necessary for assay comparison and replicability. Although individual or core laboratory capabilities play a role in selection and availability of techniques, data arising from cancer cell models are useful in guiding further research. The variety of cell lines available and the diversity of metabolic routes involved in cell responses make in vitro cell models suitable for the study of the biological effect of nanoparticles at the cell level and a valid approach for further in vivo and clinical studies. The present systematic review looks at the in vitro biological effects of different types of nanoparticles in cancer cell models.

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“…Silver NPs: gained wide application in both food and medical purposes owing to its antimicrobial activity [108] e micronucleus assay and comet assay confirmed the mutagenic and genotoxic effect of AgNPs [17,[109][110][111][112].…”

mentioning

confidence: 98%

Toxicity of Nanoparticles in Biomedical Application: Nanotoxicology

Egbuna

Parmar

Jeevanandam

et al. 2021

Journal of Toxicology

161

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Nanoparticles are of great importance in development and research because of their application in industries and biomedicine. The development of nanoparticles requires proper knowledge of their fabrication, interaction, release, distribution, target, compatibility, and functions. This review presents a comprehensive update on nanoparticles’ toxic effects, the factors underlying their toxicity, and the mechanisms by which toxicity is induced. Recent studies have found that nanoparticles may cause serious health effects when exposed to the body through ingestion, inhalation, and skin contact without caution. The extent to which toxicity is induced depends on some properties, including the nature and size of the nanoparticle, the surface area, shape, aspect ratio, surface coating, crystallinity, dissolution, and agglomeration. In all, the general mechanisms by which it causes toxicity lie on its capability to initiate the formation of reactive species, cytotoxicity, genotoxicity, and neurotoxicity, among others.

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Size of silver nanoparticles determines proliferation ability of human circulating lymphocytes in vitro

Cited by 17 publications

References 20 publications

Surface Stabilization Affects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells

Surface Stabilization Affects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells

Cytotoxic and Antiproliferative Effects of Nanomaterials on Cancer Cell Lines: A Review

Toxicity of Nanoparticles in Biomedical Application: Nanotoxicology

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