Surface Stabilization Affects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells

Vuković, Barbara; Milić, Marija; Dobrošević, Blaženka; Milić, Mirta; Ilić, Krunoslav; Pavičić, Ivan; Šerić, Vatroslav; Vrček, Ivana Vinković

doi:10.3390/nano10071390

Cited by 28 publications

(23 citation statements)

References 53 publications

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“…Cell-associated Ag (operationally defined as internalized or strongly bound) in PBMCs after 24 h of exposure to PVP-AgNPs and dissolved Ag was measured using ICP-MS after washing. For both AgNP and Ag ion exposure, the amount of Ag that was taken up by cells increased as the Ag concentration increased, which is expected and consistent with previous literature [ 49 – 50 ]. There was a significant difference between Ag that was detected in the cells (or bound strongly to the cells) after AgNO 3 treatment compared to NP treatment at concentrations of 500 and 1000 µg·L −1 , which is consistent with findings from previous studies [ 28 ].…”

Section: Discussionsupporting

confidence: 92%

Characterization, bio-uptake and toxicity of polymer-coated silver nanoparticles and their interaction with human peripheral blood mononuclear cells

Pourhoseini

Enos

Murphy

et al. 2021

Beilstein J. Nanotechnol.

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Silver nanoparticles (AgNPs) are widely used in medical applications due to their antibacterial and antiviral properties. Despite the extensive study of AgNPs, their toxicity and their effect on human health is poorly understood, as a result of issues such as poor control of NP properties and lack of proper characterization. The aim of this study was to investigate the combined characterization, bio-uptake, and toxicity of well-characterized polyvinylpyrrolidone (PVP)-coated AgNPs in exposure media during exposure time using primary human cells (peripheral blood mononuclear cells (PBMCs)). AgNPs were synthesized in-house and characterized using a multimethod approach. Results indicated the transformation of NPs in RPMI medium with a change in size and polydispersity over 24 h of exposure due to dissolution and reprecipitation. No aggregation of NPs was observed in the RPMI medium over the exposure time (24 h). A dose-dependent relationship between PBMC uptake and Ag concentration was detected for both AgNP and AgNO3 treatment. There was approximately a two-fold increase in cellular Ag uptake in the AgNO3 vs the NP treatment. Cytotoxicity, using LDH and MTS assays and based on exposure concentrations was not significantly different when comparing NPs and Ag ions. Based on differential uptake, AgNPs were more toxic after normalizing toxicity to the amount of cellular Ag uptake. Our data highlights the importance of correct synthesis, characterization, and study of transformations to obtain a better understanding of NP uptake and toxicity. Statistical analysis indicated that there might be an individual variability in response to NPs, although more research is required.

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Section: Discussionsupporting

confidence: 92%

Characterization, bio-uptake and toxicity of polymer-coated silver nanoparticles and their interaction with human peripheral blood mononuclear cells

Pourhoseini

Enos

Murphy

et al. 2021

Beilstein J. Nanotechnol.

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“…The modulation of the immune response by AgNPs is a unique area of interest because uncontrolled immune stimulation or immune suppression could lead to allergic reactions or reduce the body’s immune response to damage, infection, and cancers [ 5 ]. Currently, AgNPs have been reported to alter the morphology and viability of freshly isolated human peripheral blood mononuclear cells (hPBMCs) [ 6 ]. Moreover, pregnant mice exposed to AgNPs exhibited immunological dysfunction in the dam and placenta [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Modulation of Innate Immune Toxicity by Silver Nanoparticle Exposure and the Preventive Effects of Pterostilbene

Chen

Huang

Pranata

et al. 2021

IJMS

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Silver nanoparticles pose a potential risk to ecosystems and living organisms due to their widespread use in various fields and subsequent gradual release into the environment. Only a few studies have investigated the effects of silver nanoparticles (AgNPs) toxicity on immunological functions. Furthermore, these toxic effects have not been fully explored. Recent studies have indicated that zebrafish are considered a good alternative model for testing toxicity and for evaluating immunological toxicity. Therefore, the purpose of this study was to investigate the toxicity effects of AgNPs on innate immunity using a zebrafish model and to investigate whether the natural compound pterostilbene (PTE) could provide protection against AgNPs-induced immunotoxicity. Wild type and neutrophil- and macrophage-transgenic zebrafish lines were used in the experiments. The results indicated that the exposure to AgNPs induced toxic effects including death, malformation and the innate immune toxicity of zebrafish. In addition, AgNPs affect the number and function of neutrophils and macrophages. The expression of immune-related cytokines and chemokines was also affected. Notably, the addition of PTE could activate immune cells and promote their accumulation in injured areas in zebrafish, thereby reducing the damage caused by AgNPs. In conclusion, AgNPs may induce innate immune toxicity and PTE could ameliorate this toxicity.

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“…Indeed, many lines of evidence have pointed out that AgNPs can induce apoptosis via extrinsic and intrinsic pathways and also act as a potent activator of autophagy [2,3,24,59]. In general, smaller-sized or positively charged AgNPs are acknowledged to possess more potent apoptosis-and autophagy-inducing activities than those with bigger dimensions or negative charges [14,60,61,62]. Nonetheless, this is not always the case.…”

Section: Discussionmentioning

confidence: 99%

“…Citrate-mediated stabilization renders negative charges to AgNPs surface, while cysteaminestabilized AgNPs surface appears to be positively charged. In actual fact, differential surface charging attributed to the choice of coating agents has been shown to dictate the modalities of cell death evoked by various ENMs, inclusive of AgNPs [13,14]. Although the majority of documents have suggested that positively charged AgNPs are more toxic towards eukaryotic cells than negatively charged AgNPs, some con icting in vivo evidence exists [15,16].…”

Section: Introductionmentioning

confidence: 99%

Use of An In Silico Knowledge Discovery Approach To Translate Mechanistic Studies of Silver Nanoparticles-Induced Toxicity From In Vitro To In Vivo

Mao¹,

Luo²,

Wang³

et al. 2021

Preprint

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Background: Silver nanoparticles (AgNPs) are considered a double-edged sword that demonstrates beneficial and harmful effects depending on their dimensions and surface coating types. However, mechanistic reports regarding size- and coating-dependent effects of AgNPs remain inadequate in vitro and in vivo. In keeping with the 3Rs principles, we adopted an in silico decision tree-based knowledge-discovery-in-databases process to offer a multilayered view of AgNPs toxicity and translate our research from cell-based phenomenological observations to further in vitro and in vivo mechanistic explorations.Results: Cell viability assessment results was used to create a tree model for predicting toxicity evoked by four AgNP types: SCS, LCS, SAS, and LAS. This model ranked toxicity-relevant parameters in the order: dose > cell type > AgNP type ≥ exposure time. As suggested by the model, we chose a less responsive cell line to conduct further investigations. LCS among others was more capable of comprising viability of this cell line and could trigger higher levels of apoptosis and autophagy at a subcytotoxic dose. Even though at a cytotoxic dose, it was yet unable to evoke necrosis. Longer exposure to a noncytotoxic dose of LCS induced G2/M cell cycle arrest and senescence rather than eliciting apoptosis and autophagy. After a single intraperitoneal injection, SCS was found to be more toxic to mice than SAS, both of which could be deposited in various target organs (e.g., spleen, liver, and kidneys). Morphological observation, together with serum biochemical and histological analyses, indicated that AgNPs could produce pancreatic toxicity, apart from leading to hepatic inflammation. Conclusions: Our integrated in vitro, in silico, and in vivo study demonstrated that AgNPs could exert toxicity in dose-, cell/organ type- and particle type-dependent manners. More importantly, a single injection of lethal-dose AgNPs (i.e., SCS and SAS) could incur severe damage to pancreas and raise blood glucose levels at the early phase of exposure.

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Surface Stabilization Affects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells

Cited by 28 publications

References 53 publications

Characterization, bio-uptake and toxicity of polymer-coated silver nanoparticles and their interaction with human peripheral blood mononuclear cells

Characterization, bio-uptake and toxicity of polymer-coated silver nanoparticles and their interaction with human peripheral blood mononuclear cells

Modulation of Innate Immune Toxicity by Silver Nanoparticle Exposure and the Preventive Effects of Pterostilbene

Use of An In Silico Knowledge Discovery Approach To Translate Mechanistic Studies of Silver Nanoparticles-Induced Toxicity From In Vitro To In Vivo

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