Size Exclusion Chromatography of Poly(vinylpyrrolidone): I. The Chromatographic Method

Malawer, E. G.; DeVasto, Joseph K.; Frankoski, Stanley P.; Montana, Anthony J.

doi:10.1080/01483918408073978

Cited by 11 publications

(5 citation statements)

References 12 publications

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“…PVP has also been used as stationary phase materials or extractant for both extractions and chromatography (16)(17)(18)(19). SEC determinations of povidone have focused on molecular weight characterization of the povidone itself or the use of povidone as a molecular weight calibrator for other determinations (20)(21)(22)(23)(24)(25)(26)(27)(28).…”

Section: Introductionmentioning

confidence: 99%

Determination of Total Polyvinylpyrrolidone (PVP) in Ophthalmic Solutions by Size Exclusion Chromatography with Ultraviolet-visible Detection

Tavlarakis¹,

Urban

Snow

2011

Journal of Chromatographic Science

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A straightforward size exclusion chromatography (SEC) method was developed and validated for the determination of total polivinylpyrrolidone (PVP) in ophthalmic solutions using the unusual combination of size exclusion chromatography (SEC), ultraviolet-visible detection and quantitation of an analyte peak that elutes in the total exclusion volume of the column. Samples of opthalmic solutions are diluted with water and injected onto a TSKgel G1000PW, 7.5 mm i.d. × 30 cm, 12 μm column at 50°C, with 80:20 0.1M sodium acetate-methanol mobile phase and UV detection at 220 nm. Validation was successful for a stability indicating pharmaceutical method, with parameters including specificity, accuracy, linearity, and precision within typical pharmaceutical acceptance criteria. A stress study with acid, base, peroxide, heat, and light indicates that there is no interference from drug, product, or excipients.

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Section: Introductionmentioning

confidence: 99%

Determination of Total Polyvinylpyrrolidone (PVP) in Ophthalmic Solutions by Size Exclusion Chromatography with Ultraviolet-visible Detection

Tavlarakis¹,

Urban

Snow

2011

Journal of Chromatographic Science

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“…Size-exclusion chromatography (SEC) is an exclusive technique used to determine the molecular mass of polymers . For this purpose, diol-derivatized silica gel was used as a stationary phase packed in a glass column (9 × 2 cm).…”

Section: Methodsmentioning

confidence: 99%

“…the molecular mass of polymers. 12 For this purpose, diol-derivatized silica gel was used as a stationary phase packed in a glass column (9 × 2 cm). The packed column was washed with a methanol/water (1:1) mixture as a mobile phase.…”

Section: Determination Of Molecular Mass Size-exclusion Chromatograph...mentioning

confidence: 99%

Thiolated PVP–Amphotericin B Complexes: An Innovative Approach toward Highly Mucoadhesive Gels for Mucosal Leishmaniasis Treatment

et al. 2020

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The aim of this study was to synthesize polymeric excipients that can form mucoadhesive hydrogels containing amphotericin B (AmB) for the treatment of mucosal leishmaniasis. 2-(2-Acryloylaminoethyldisulfanyl)-nicotinic acid (ACENA) was copolymerized with N-vinyl pyrrolidone to obtain thiolated polyvinylpyrrolidone (PVP) that was then complexed with AmB to improve its solubility. The resulting structure of thiolated PVP was evaluated by 1H nuclear magnetic resonance to confirm S-protected thiol groups, and the average molecular mass was determined by size exclusion chromatography. Moreover, variants of thiolated PVP–AmB were studied for the thiol content, amount of complexed AmB, cytotoxicity, mucoadhesive properties, and antileishmaniasis activity. The highest achieved degree of thiolation was 772 ± 24.64 μmol/g, and the amount of complexed AmB was 27.05 ± 0.31 μmol per g of polymer. Thiolated PVP and thiolated PVP–AmB variants (0.5% m/v) showed no cytotoxicity, whereas the equivalent concentration of free AmB reduced Caco-2 cell viability to 70% within 24 h. Thiol-functionalized PVP and PVP–AmB complexes displayed 7.66- and 7.20-fold higher adhesion to the mucosal surface in comparison to unmodified PVP and PVP–AmB, respectively. In addition, variants of thiolated PVP–AmB complexes displayed 100% antileishmaniasis activity in comparison to the 80% killing efficiency of Fungizone, which has been applied in the equivalent AmB concentration of 0.2 μg/mL. Thiol-functionalized PVP proved to be a promising novel excipient for the delivery of AmB providing enhanced solubility and improved mucoadhesive properties which are beneficial for the treatment of mucosal leishmaniasis.

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“…The molecular mass of thiolated PVP was determined by size exclusion chromatography. 12 Detection of the polymer eluting from the column was based on the principle of developing a yellow to brown color when iodine reacts with the pyrrolidone group of the polymer.…”

Section: Methodsmentioning

confidence: 99%

Synthesis and Characterization of Thiolated PVP–Iodine Complexes: Key to Highly Mucoadhesive Antimicrobial Gels

Jalil

Matuszczak

et al. 2018

Mol. Pharmaceutics

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The aim of this study was to synthesize iodine containing polymeric excipients for mucosal treatment of microbial infection exhibiting a prolonged mucosal residence time by forming an adhesive gel on the mucosal surface. In order to achieve this aim, 2-(2 acryloylamino-ethyldisulfanyl)-nicotinic acid (ACENA) was copolymerized with N-vinylpyrrolidone (NVP) to obtain thiolated polyvinylpyrrolidone (PVP) for complexation with iodine. The average molecular mass of different thiolated PVP variants was determined by size exclusion chromatography. The structure of thiolated PVP was confirmed by H NMR. Thiolated PVP variants were characterized for thiol content, cytotoxicity, iodine loading capacity, rheological behavior, and adhesion time on mucosa. The highest achieved degree of thiolation was 610 ± 43 μmol/g, and the maximum recorded iodine loading was 949 ± 31 μmol/g of polymer. Thiolated PVP variants (0.5% m/v) showed no toxicity after incubation on Caco-2 cells for the period of 3 and 24 h, respectively. Thiolated PVP and thiolated PVP-iodine complexes exhibited a 5.4- and 4.4-fold increased dynamic viscosity in porcine mucus in comparison to PVP and PVP-iodine complex, respectively. Compared to PVP and PVP-iodine complex thiol-functionalized PVP and PVP-iodine complexes demonstrated significantly prolonged attachment to mucosal surface over a period of 3 h. Thiol functionalized PVP proved to be a promising novel excipient for complexation with iodine and to exhibit strongly improved mucoadhesive properties.

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Size Exclusion Chromatography of Poly(vinylpyrrolidone): I. The Chromatographic Method

Cited by 11 publications

References 12 publications

Determination of Total Polyvinylpyrrolidone (PVP) in Ophthalmic Solutions by Size Exclusion Chromatography with Ultraviolet-visible Detection

Determination of Total Polyvinylpyrrolidone (PVP) in Ophthalmic Solutions by Size Exclusion Chromatography with Ultraviolet-visible Detection

Thiolated PVP–Amphotericin B Complexes: An Innovative Approach toward Highly Mucoadhesive Gels for Mucosal Leishmaniasis Treatment

Synthesis and Characterization of Thiolated PVP–Iodine Complexes: Key to Highly Mucoadhesive Antimicrobial Gels

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