Size dependent immune response after subcutaneous, oral and intranasal administration of BSA loaded nanospheres

Gutierro, I.; Hernández, Rosa María; Igartua, Manoli; Rodríguez-Gascón, Alicia; Pedraz, José Luís

doi:10.1016/s0264-410x(02)00435-8

Cited by 264 publications

(149 citation statements)

References 33 publications

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“…For the optimisation of the MS, the formulations were prepared using 0.1% rhEGF instead of using a model protein, as previously reported in other studies. (Gutierro, et al 2002). This delivery system has the advantages of a polymeric therapeutic approach, including biocompatibility, biodegradability and allows a controlled and sustained release of the entrapped drug (Jain 2000;Tessmar and Göpferich 2007).…”

Section: Discussionmentioning

confidence: 99%

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rhEGF-loaded PLGA-Alginate microspheres enhance the healing of full-thickness excisional wounds in diabetised Wistar rats

Gainza

Aguirre

Pedraz

et al. 2013

European Journal of Pharmaceutical Sciences

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Diabetic foot ulcers (DFUs) represent a major clinical challenge in the ageing population. To address this problem, rhEGF-loaded Poly-Lactic-co-Glycolic-Acid (PLGA)-Alginate microspheres (MS) were prepared by a modified w/o/w-doubleemulsion/solvent evaporation method. Different formulations were evaluated with the aim of optimising MSs properties by adding NaCl to the surfactant solution and/or the solvent removal phase and adding alginate as a second polymer. The characterization of the developed MS showed that alginate incorporation increased the encapsulation efficiency (EE) and NaCl besides increasing the EE also became the particle surface smooth and regular. Once the MS were optimised, the target loading of rhEGF was increased to 1% (PLGA-Alginate MS), and particles were sterilised by gamma radiation to provide the correct dosage for in vivo studies. In vitro cell culture assays demonstrated that neither the microencapsulation nor the sterilisation process affected rhEGF bioactivity or rhEGF wound contraction. Finally, the MS were evaluated in vivo for treatment of the full-thickness wound model in diabetised Wistar rats. rhEGF MS treated animals showed a statistically significant decrease of the wound area by days 7 and 11, a complete re-epithelisation by day 11 and an earlier resolution of the inflammatory process. Overall, these findings demonstrate the promising potential of rhEGF-loaded MS (PLGA-Alginate MS) to promote faster and more effective wound healing, and suggest its possible application in DFU treatment.

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Section: Discussionmentioning

confidence: 99%

“…The release medium was removed by centrifugation and replaced by the same quantity of PBS at predetermined intervals (Gutierro, et al 2002). Samples were taken at 30 min., 4, 8 and 10 h. Afterwards, once a day during the first week, and once a week up to 41 days.…”

Section: In Vitro Release Studiesmentioning

confidence: 99%

rhEGF-loaded PLGA-Alginate microspheres enhance the healing of full-thickness excisional wounds in diabetised Wistar rats

Gainza

Aguirre

Pedraz

et al. 2013

European Journal of Pharmaceutical Sciences

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“…Na análise de anticorpos, estabeleceu-se um protocolo de ELISA, baseado em outros trabalhos, utilizando-se albumina sérica bovina como antígeno (Parmentier et al, 1994;Bar-Shira et al, 2002;Gutierro et al, 2002). Os soros das aves foram analisados individualmente em duplicata e os resultados foram as médias das densidades óticas determinadas em comprimento de onda de 495 nm em espectrofotômetro Titertek Multiscan MC.…”

Section: Methodsunclassified

Suplementação de vitaminas e minerais orgânicos e sua ação sobre a imunocompetência de frangos de corte submetidos a estresse por calor

et al. 2008

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RESUMO -Estudou-se o efeito de dietas suplementadas com vitaminas C e E e com minerais orgânicos Zn e Se sobre parâmetros imunológicos de frangos de corte de 1 a 35 dias de idade submetidos a estresse cíclico por calor (25 a 32 o C). Avaliou-se ainda a inoculação com albumina sérica bovina (BSA) como estratégia para medir a imunidade humoral. Utilizaram-se 272 aves Ross para avaliação de quatro tipos de suplementação vitamínico-mineral (SVM): D1 -dieta controle com 60 e 30 UI de vit. E/kg de ração nas rações inicial e de crescimento, respectivamente; 0 de vit. C, 80 ppm de Zn inorgânico e 0,3 ppm de Se inorgânico/kg de ração; D2 -dieta controle + 100 UI vit. E e 300 ppm vit. C/kg de ração; D3 -dieta controle + 40 ppm Zn e 0,3 ppm Se orgânicos/kg de ração; D4 -dieta controle + níveis de SVM utilizados em D2 e D3; e de dois ambientes: termoneutro (ATN) e estresse cíclico pelo calor (EPC) a partir dos 14 dias de idade. Seis e cinco aves por tipo de suplementação no EPC e ATN, respectivamente, foram inoculadas com BSA aos 12 e aos 24 dias de idade. O soro das aves com 35 dias de idade foi analisado por ELISA. A suplementação de vitaminas e minerais não influenciou a produção de anticorpos de frangos de corte desafiados com BSA. O estresse por calor provocou maior produção de anticorpos anti-BSA, independentemente da dieta utilizada. A inoculação com BSA não influenciou o desempenho das aves e foi uma estratégia eficiente para avaliar a imunidade humoral de frangos, visto que as aves responderam ao seu desafio com aumento na produção de anticorpos e no tamanho de bursa, apesar da grande variabilidade individual.Palavras-chave: imunomodulação nutricional, selênio, vitamina C, vitamina E, zinco Vitamins and organic minerals supplementation and its effect upon the immunocompetence of broilers submitted to heat stress ABSTRACT -The objective of this experiment was to evaluate the effect of diet supplementation with vitamins C and E and organic minerals Zn and Se on immunological parameters of broilers from 1 to 35 days, kept on cyclic heat stress (25 to 32°C) and to evaluate the usefulness of bovine serum albumin (BSA) inoculation to determine humoral immunity. A total of 272 Ross broilers were used to evaluate four types of vitamin-mineral supplementation (VMS): D1-control diet with 60 and 30 IU of vit E for starter and growing diet, respectively, zero vit C, 80 ppm of inorganic Zn, 0.3 ppm of inorganic Se; D2 -control diet + 100 UI vit E and 300 ppm vit C/kg; D3 -control diet + 40 ppm Zn and 0.3 ppm Se/kg, both from organic sources; D4 -control diet and levels of VMS used in D2 and D3), and two environments: thermoneutral (TNA) and cyclic heat stress (CHS) from 14 days of age. Six and five birds per type of supplementation in CHS and TNA, respectively, were inoculated with BSA at 12 and 24 days. Birds serum at 35 days-old was analyzed by ELISA. Supplementation with vitamins and minerals did not influence antibody production of broilers challenged with BSA. Heat stress improved antibody anti-BSA production regardless of...

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“…[16] This effect may be attributed to preferential uptake of microparticles by microfold cells (M-cells) or to premature antigen release from the delivery system, which could be more pronounced for nanoparticles because of their higher surface-to-volume ratios as compared to microparticles.…”

Section: Size Mattersmentioning

confidence: 99%

“…[18] Ironically, whereas most vaccines, including live or killed bacteria and viruses as well as subunit vaccines, are based on particles (see Figure 1), in vaccine research relatively little attention is being paid to a rigorous particle characterization with respect to size and shape. Typically, when a nanoparticulate vaccine formulation is prepared, the particle size is confirmed by, for example, dynamic light scattering (DLS) [11,16] and the formulation scientist may be satisfied when indeed nanoparticles with a narrow size distribution are detected. However, DLS is a low-resolution technique and, in addition, does not provide information about particle content and shape.…”

Section: Sizing Mattersmentioning

confidence: 99%

Sizing the optimal dimensions of a vaccine delivery system: a particulate matter

Slütter

Jiskoot

2015

Expert Opinion on Drug Delivery

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Size dependent immune response after subcutaneous, oral and intranasal administration of BSA loaded nanospheres

Cited by 264 publications

References 33 publications

rhEGF-loaded PLGA-Alginate microspheres enhance the healing of full-thickness excisional wounds in diabetised Wistar rats

rhEGF-loaded PLGA-Alginate microspheres enhance the healing of full-thickness excisional wounds in diabetised Wistar rats

Suplementação de vitaminas e minerais orgânicos e sua ação sobre a imunocompetência de frangos de corte submetidos a estresse por calor

Sizing the optimal dimensions of a vaccine delivery system: a particulate matter

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