Size control of hollow poly-allylamine hydrochloride/poly-sodium styrene sulfonate microcapsules using the bubble template method

Molino, Jay; Matsuoka, Eitaro; Daiguji, Hirofumi

doi:10.1039/c0sm00867b

Cited by 15 publications

(13 citation statements)

References 26 publications

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“…The formation and application of such films has been reported by many authors . The systems for biomedical applications include superhydrophobic coatings, surfaces of modulated protein and cell adhesion, hollow microcapsules for drug delivery and ultrasound contrast agent carriers, nonviral transfection agents, or magnetic microgels for magnetic resonance imaging . Recently, PEMs containing PAH and various polyanions were used to prepare “active” wound care dressings.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of strong polycations with improved biological properties

Wytrwał-Sarna

Koczurkiewicz

Wójcik

et al. 2013

J Biomedical Materials Res

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Poly(allylamine hydrochloride) (PAH) has found many applications both in biotechnology and biomedical fields. However, its high toxicity toward various mammalian cells significantly limits its effective usage. This study focuses on improving the biological properties of PAH by its modification to strong polyelectrolytes. The strong polycations were prepared by the direct quaternization of PAH amino groups or by the attachment of glycidyltrimethylammonium chloride to these groups. The biological properties, such as cytotoxicity toward human skin fibroblasts (HSFs), proliferation and migration of the cells on a polymeric surface, and antibacterial activities against two pathogenic bacteria, Staphylococcus aureus and Escherichia coli, were determined. All the modified polyelectrolytes are considerably less toxic to HSFs as compared to PAH. Moreover, the directly quaternized polycations are stronger biocides against S. aureus than the parent polymer. Contrary to PAH, thin films of the modified polyelectrolytes improve or do not affect HSFs proliferation and can stimulate cell migration into the wound, as was demonstrated using an in vitro model. The relationship between the structure of the modified polymers (amount and localization of the quaternary ammonium groups) and the biological activity is discussed. Due to the improved biological properties, the obtained polycations may be potentially useful for a variety of biotechnological and biomedical applications.

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Section: Introductionmentioning

confidence: 99%

Synthesis of strong polycations with improved biological properties

Wytrwał-Sarna

Koczurkiewicz

Wójcik

et al. 2013

J Biomedical Materials Res

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“…Varieties of microbubble formation and control techniques have been developed [3][4][5][6][7][8][9][10][11] and utilized in different applications. [12][13][14][15][16][17][18][19][20][21] For other cases, some unwanted microbubbles are accused as one of the sources of performance deterioration, and various bubble removal strategies have been developed. [22][23][24][25][26][27][28] Unlike the segmented flow or the bubble clusters, the air bubble generated on the microchannel walls or surfaces have attracted much attention.…”

Section: Introductionmentioning

confidence: 99%

Tunable microfluidic standing air bubbles and its application in acoustic microstreaming

Liu

Zhu

et al. 2019

Biomicrofluidics

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Microbubbles are often used in chemistry, biophysics, and medicine. Properly controlled microbubbles have been proved beneficial for various applications by previous scientific endeavors. However, there is still a plenty of room for further development of efficient microbubble handling methods. Here, this paper introduces a tunable, stable, and robust microbubble interface handling mechanism, named as microfluidic standing air bubbles (μSABs), by studying the multiphysical phenomena behind the gas-liquid interface formation and variation. A basic μSAB system consists specially structured fluidic channels, pneumatic channels, and selectively permeable porous barriers between them. The μSABs originate inside the crevice structures on the fluidic channel walls in a repeatable and robust manner. The volumetric variation of the μSAB is a multiphysical phenomenon that dominated by the air diffusion between the pneumatic channel and the bubble. Theoretical analysis and experimental data illustrate the coupling processes of the repeatable and linear μSAB volumetric variation when operated under common handling conditions (control pneumatic pressure: −90 kPa to 200 kPa). Furthermore, an adjustable acoustic microstreaming is demonstrated as an application using the alterable μSAB gas-liquid interface. Derived equations and microscopic observations elucidate the mechanism of the continuous and linear regulation of the acoustic microstreaming using varying μSAB gas-liquid interfaces. The μSAB system provides a new tool to handle the flexible and controllable gas-liquid interfaces in a repeatable and robust manner, which makes it a promising candidate for innovative biochemical, biophysical, and medical applications.

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“…The dispersed microbubbles have been found to be unstable due to the effects of their surface tension: the microbubbles tend to leak into aqueous solutions due to a large Laplace pressure that drives the dissolution of the bubbles [7,8], and they need to be covered with a variety of stabilizing shells [1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23]. The diffusion of the gas can be slowed by coating the gas core, thereby increasing the lifetime to a few months [4,5,15].…”

Section: Introductionmentioning

confidence: 99%

“…One of the most promising shells for this purpose is the polyelectrolyte (PE) multi-layer formed by the layer-by-layer (LbL) method [2,9,10,11,12,15,17,18]. The LbL technique is essentially based on charge reversal phenomena whereby colloidal particles are overcharged by adding oppositely charged PEs [26,27].…”

Section: Introductionmentioning

confidence: 99%

“…While there was a previous report that used CO 2 microbubbles for the preparation of multi-layers of pure PEs [15,17,18], it has been suggested that uncoated microbubbles of air or oxygen have some difficulty in implementing the charge reversal caused by cationic PEs (polycations) that violates the electrical stoichiometry [26,27], though a wide variety of colloid-PE complexations have almost satisfied the stoichiometry at the isoelectric point [44]. We have ascribed the anomalous phenomena to the anions on the surface of unmodified air or oxygen microbubbles that arise from the adsorption of hydroxyl ions on the gas surface [26,27], except for in strong acid solutions [1,45,46]; however, the details remain to be addressed, and we need to investigate the monolayer deposition regarding various species of polycations and compare the first and second charge reversals due to the depositions of mono-and double-layers, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Microscopic Characterization of Individual Submicron Bubbles during the Layer-by-Layer Deposition: Towards Creating Smart Agents

Kato

Frusawa

2015

Materials

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We investigated the individual properties of various polyion-coated bubbles with a mean diameter ranging from 300 to 500 nm. Dark field microscopy allows one to track the individual particles of the submicron bubbles (SBs) encapsulated by the layer-by-layer (LbL) deposition of cationic and anionic polyelectrolytes (PEs). Our focus is on the two-step charge reversals of PE-SB complexes: the first is a reversal from negatively charged bare SBs with no PEs added to positive SBs encapsulated by polycations (monolayer deposition), and the second is overcharging into negatively charged PE-SB complexes due to the subsequent addition of polyanions (double-layer deposition). The details of these phenomena have been clarified through the analysis of a number of trajectories of various PE-SB complexes that experience either Brownian motion or electrophoresis. The contrasted results obtained from the analysis were as follows: an amount in excess of the stoichiometric ratio of the cationic polymers was required for the first charge-reversal, whereas the stoichiometric addition of the polyanions lead to the electrical neutralization of the PE-SB complex particles. The recovery of the stoichiometry in the double-layer deposition paves the way for fabricating multi-layered SBs encapsulated solely with anionic and cationic PEs, which provides a simple protocol to create smart agents for either drug delivery or ultrasound contrast imaging.

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Size control of hollow poly-allylamine hydrochloride/poly-sodium styrene sulfonate microcapsules using the bubble template method

Cited by 15 publications

References 26 publications

Synthesis of strong polycations with improved biological properties

Synthesis of strong polycations with improved biological properties

Tunable microfluidic standing air bubbles and its application in acoustic microstreaming

Microscopic Characterization of Individual Submicron Bubbles during the Layer-by-Layer Deposition: Towards Creating Smart Agents

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