Size and stability of liposomes: A possible role of hydration and osmotic forces

Sabín, Juan; Prieto, Gerardo; Ruso, Juan M.; Hidalgo‐Álvarez, R.; Sarmiento, Félix

doi:10.1140/epje/i2006-10029-9

Cited by 130 publications

(105 citation statements)

References 47 publications

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“…The mean±SD of vesicle diameter determined at 3, 6, 9, and 12 months was 720±30, 750±40, 640±40, and 400±40 nm, respectively (Table I). Vesicle shrinkage has been attributed to water evacuation until maximum compaction of vesicles with possible implication of difference in osmotic forces on both sides of the bilayer (48). In the present study, movement of VCM from the bilayer to the outer niosome surface during storage possibly created an osmotic force without affecting the EE equilibrium.…”

Section: Characteristics Of Niosomesmentioning

confidence: 58%

Vancomycin-Eluting Niosomes: A New Approach to the Inhibition of Staphylococcal Biofilm on Abiotic Surfaces

et al. 2014

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Abstract. A new vancomycin (VCM)-eluting mixed bilayer niosome formulation was evaluated for the control of staphylococcal colonization and biofilm formation on abiotic surfaces, a niosome application not explored to date. Cosurfactant niosomes were prepared using a Span 60/Tween 40/cholesterol blend (1: 1: 2). Tween 40, a polyethoxylated amphiphile, was included to enhance VCM entrapment and confer niosomal surface properties precluding bacterial adhesion. VCM-eluting niosomes showed good quality attributes including relatively high entrapment efficiency (∼50%), association of Tween 40 with vesicles in a constant proportion (∼87%), biphasic release profile suitable for inhibiting early bacterial colonization, and long-term stability at 4°C for a 12-month study period. Niosomes significantly enhanced VCM activity against planktonic bacteria of nine staphylococcal strains. Using microtiter plates as abiotic surface, VCM-eluting niosomes proved superior to VCM in inhibiting biofilm formation, eradicating surface-borne biofilms, inhibiting biofilm growth, and interfering with biofilm induction by VCM subminimal inhibitory concentrations. Data suggest dual functionality of cosurfactant VCM-eluting niosomes as passive colonization inhibiting barrier and active antimicrobial-controlled delivery system, two functions recognized in infection control of abiotic surfaces and medical devices.

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Section: Characteristics Of Niosomesmentioning

confidence: 58%

Vancomycin-Eluting Niosomes: A New Approach to the Inhibition of Staphylococcal Biofilm on Abiotic Surfaces

et al. 2014

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“…Colloidal stability of liposomes systems can be explained by the Derjaguin-Landau-Verwey-Overbeek (DLVO) theory, which describes that the stability of the colloidal systems is determined by the net effect of repulsive electrical double layer and the attractive van der Waals forces that the particles experience as they contact one another (Sabín et al, 2006). Factors like adsorption of proteins and neutralization of surface charges promote van der Waal's associative forces and reduce the repulsive forces.…”

Section: Discussionmentioning

confidence: 99%

EpCAM-targeted liposomal si-RNA delivery for treatment of epithelial cancer

et al. 2014

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Background: RNA interference (RNAi) technology using short interfering RNA (si-RNA) has shown immense potential in the treatment of cancers through silencing of specific genes. Cationic non-viral vectors employed for gene delivery exhibit toxic effects in normal cells limiting their widespread use, therefore, site-specific delivery using benign carriers could address this issue. Objective: Design of a non-toxic carrier that enables site-specific delivery of si-RNA into the cancer cells is of prime importance to realize the promise of gene silencing. Methods: In the present study, non-cationic liposomes encapsulating si-RNA against epithelial cell adhesion molecule (EpCAM) were developed and characterized for encapsulation efficiency, colloidal stability, in vitro and in vivo gene silencing efficacy. Results: PEGylated liposomes containing phosphatidyl choline and phosphatidyl ethanolamine exhibited maximum si-RNA encapsulation efficiency of 47%, zeta potential of -21 mV, phase transition temperature of 51 C and good colloidal stability in phosphate-buffered saline (PBS) containing bovine serum albumin (BSA) and plasma protein (PP) at 37 C. Conjugation of epithelial cell adhesion molecule (EpCAM) antibody to the liposomes resulted in enhanced cell internalization and superior down-regulation of EpCAM gene in MCF-7 cell lines when compared with free si-RNA and the non-targeted liposomes. In vivo evaluation of immunoliposomes for their efficacy in regressing the tumor volume in Balb/c SCID mice showed about 35% reduction of tumor volume in comparison with the positive control when administered with an extremely low dose of 0.15 mg/kg twice a week for 4 weeks. Conclusion: Our results exhibit that the nanocarrier-mediated silencing of EpCAM gene is a promising strategy to treat epithelial cancers.

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“…Liposomes are synthetically prepared, spherical, self-enclosed microscopic entities with membrane bilayer-like structures 2 . They are formed by exposing the lipid headgroups to a strongly aqueous environment 3 . Use of such systems for different modes of administration is advantageous because of their biocompatibility and biodegradability.…”

Section: Introductionmentioning

confidence: 99%

Influence of Lipid Composition, pH, and Temperature on Physicochemical Properties of Liposomes with Curcumin as Model Drug

et al. 2016

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Size and stability of liposomes: A possible role of hydration and osmotic forces

Cited by 130 publications

References 47 publications

Vancomycin-Eluting Niosomes: A New Approach to the Inhibition of Staphylococcal Biofilm on Abiotic Surfaces

Vancomycin-Eluting Niosomes: A New Approach to the Inhibition of Staphylococcal Biofilm on Abiotic Surfaces

EpCAM-targeted liposomal si-RNA delivery for treatment of epithelial cancer

Influence of Lipid Composition, pH, and Temperature on Physicochemical Properties of Liposomes with Curcumin as Model Drug

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