Size and location of the transforming region in human adenovirus type 5 DNA

Graham, Frank L.; Eb, A. J. Van Der; Heijneker, H. L.

doi:10.1038/251687a0

Cited by 235 publications

(116 citation statements)

References 21 publications

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“…together with E1B or a number of other viral and cellular oncogenes can transform cells to an oncogenic state (9,43,49). ElA alone can generate immortalized cell lines from primary cultures (14).…”

Section: Simon Et Al In Preparation)mentioning

confidence: 99%

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Selective induction of human heat shock gene transcription by the adenovirus E1A gene products, including the 12S E1A product.

Simon¹,

Kitchener²,

Kao³

et al. 1987

Mol. Cell. Biol.

111

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We have previously shown that the human 70-kilodalton heat shock protein gene (hsp7O) is induced by the adenovirus ElA gene product and during the S-G2 phase of the cell cycle. In this study, we investigated the effect of ElA on the expression of other human hsp genes. A gene encoding one form of the hsp89 protein (hsp89a) was activated during an adenovirus infection with kinetics similar to those of activation of hsp7O. The induction required a functional ElA gene. However, the hsp89 transcript was not cell cycle regulated. Genes encoding another form of hsp89 and the hsp27 protein were not induced by ElA or during the cell cycle. Further examination of hsp7O expression revealed a greater complexity than previously seen. Si nuclease analysis using an hsp70 cDNA as well as a distinct hsp7O genomic clone demonstrated three related hsp70 transcripts; two were induced by ElA, and one was not. Both of the ElA-inducible genes were regulated during the cell cycle. All three were induced by heat shock. These results suggest common aspects of control among certain members of this family of cellular genes distinct from heat shock control. Finally, using viruses that express the individual ElA proteins, we found that the hsp70 gene is induced by the 12S and the 13S ElA products. The efficiency of induction by the 12S product was somewhat less than that by the 13S product but only by a factor of less than 2. This is in contrast to the induction of early viral genes, for which the 13S product is considerably more efficient than the 12S product.

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Section: Simon Et Al In Preparation)mentioning

confidence: 99%

“…Introduction of the ElA gene into primary cell cultures can lead to immortalization (14). In combination with E1B or other oncogenes, ElA expression can transform cells to an oncogenic state (9,23,43,49). Although a cellular counterpart of the ElA gene has not been identified, there is * Corresponding author.…”

mentioning

confidence: 99%

Selective induction of human heat shock gene transcription by the adenovirus E1A gene products, including the 12S E1A product.

Simon¹,

Kitchener²,

Kao³

et al. 1987

Mol. Cell. Biol.

111

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“…Later, cell lines containing defined enzymatic defects and setectable systems were established, and thus, started the era of gene transfer (1). Later developments specialized a method of calcium phosphate-mediated transfection which became widely used (12).…”

Section: Historymentioning

confidence: 99%

Genetic engineering: Moral aspects and control of practice

Eisenberg

Schenker

1997

J Assist Reprod Genet

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“…Human adenovirus serotypes 18 and 31 were prototype strains and were obtained from Flow Laboratories (Rockville, Md.) and H. Shimojo, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Although the arrangement of sequences within the repetition should be apparent from the morphology of the closure region, in the case of adenovirus DNA this region apparently has been too short to visualize by electron microscopy. We have recently found, however, that preparations of denatured human adenovirus type 18 (Ad 18) DNA can yield up to 80% single-stranded circular molecules which contain a visible projection or "panhandle." In the present report we show that these projections are duplex structures and that they are produced by annealing of the inverted terminal repetition with corresponding complementary sequences at the opposite end of the strand.…”

mentioning

confidence: 99%

Arrangement of sequences in the inverted terminal repetition of adenovirus 18 DNA.

Garon¹,

Berry²,

Rose³

1975

Proc. Natl. Acad. Sci. U.S.A.

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In contrast to the single-stranded circular molecules produced with denatured DNA from other adenoviruses, there was associated with nearly all circular molecules of adenovirus type 18 a visible, duplex projection.These projections had a mean contour length of 0.31 ± 0.12 ,sm, equivalent to approximately 3% of genome length. Individual (3)(4)(5). At present the DNA of only one other group of viruses, the adenovirus-associated viruses (AAV), has been found to contain a similar type of repetition (6). Because of these repetitious sequences, the ends of single-stranded DNA molecules can self-anneal to form circles closed by a duplex hydrogenbonded segment. Although the arrangement of sequences within the repetition should be apparent from the morphology of the closure region, in the case of adenovirus DNA this region apparently has been too short to visualize by electron microscopy. We have recently found, however, that preparations of denatured human adenovirus type 18 (Ad 18) DNA can yield up to 80% single-stranded circular molecules which contain a visible projection or "panhandle." In the present report we show that these projections are duplex structures and that they are produced by annealing of the inverted terminal repetition with corresponding complementary sequences at the opposite end of the strand. They thus represent the hydrogen-bonded segments closing circular molecules and consequently provide direct, visual evidence for the arrangement of sequences within the inverted terminal repetition of an adenovirus DNA. MATERIALS AND METHODSViruses and Viral DNA. Human adenovirus serotypes 18 and 31 were prototype strains and were obtained from Flow Laboratories (Rockville, Md.) and H. Shimojo, respectively. Both viruses had been plaque-purified and were free of AAV contamination. Stock pools were prepared by passage in primary human embryonic kidney cells (HEM Research, Inc., Rockville, Md.). Viruses were produced in suspension cultures of KB cells and purified as described (7). The extraction of intact adenovirus DNA from bands of CsCl-purified virus was carried out as before (8)

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Size and location of the transforming region in human adenovirus type 5 DNA

Cited by 235 publications

References 21 publications

Selective induction of human heat shock gene transcription by the adenovirus E1A gene products, including the 12S E1A product.

Selective induction of human heat shock gene transcription by the adenovirus E1A gene products, including the 12S E1A product.

Genetic engineering: Moral aspects and control of practice

Arrangement of sequences in the inverted terminal repetition of adenovirus 18 DNA.

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