Six new dammarane-type triterpenes from acidic hydrolysate of the stems-leaves of Panax ginseng and their inhibitory–activities against three human cancer cell lines

Ma, Lei; Yang, Xiu‐Wei

doi:10.1016/j.phytol.2015.08.002

Cited by 20 publications

(9 citation statements)

References 27 publications

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“…Due to the influence of the factors such as environment and cultivation techniques, quality of GRR varies from different sources. Consensus opinion [1] suggests that the main bioactive principles of GRR are ginsenosides (ginseng saponins), derivatives of the triterpene dammarane and/or oleanolic acid structures, especially dammarane ones, which exhibit properties of anti-cancer [2,3,4,5], neuroprotective [5,6,7,8,9], anti-oxidant [5,10,11,12,13], hepatoprotective [5,14], anti-nociception [5,15], anti-inflammatory, anti-stress, hypoglycemic, anti-fatigue [5], melanogenesis inhibitory [16], and silent information regulator two homolog 1 activator [17] effects. In addition, some ginsenosides and their aglycone exhibit good pharmacokinetic properties [18,19].…”

Section: Introductionmentioning

confidence: 99%

High-Performance Liquid Chromatography with Diode Array Detector and Electrospray Ionization Ion Trap Time-of-Flight Tandem Mass Spectrometry to Evaluate Ginseng Roots and Rhizomes from Different Regions

et al. 2016

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Ginseng, Panax ginseng C. A. Meyer, is an industrial crop in China and Korea. The functional components in ginseng roots and rhizomes are characteristic ginsenosides. This work developed a new high-performance liquid chromatography coupled with electrospray ionization ion trap time-of-flight multistage mass spectrometry (LC-ESI-IT-TOF-MS n ) method to identify the triterpenoids. Sixty compounds (1-60) including 58 triterpenoids were identified from the ginseng cultivated in China. Substances 1, 2, 7, 15-20, 35, 39, 45-47, 49, 55-57, 59, and 60 were identified for the first time. To evaluate the quality of ginseng cultivated in Northeast China, this paper developed a practical liquid chromatography-diode array detection (LC-DAD) method to simultaneously quantify 14 interesting ginsenosides in ginseng collected from 66 different producing areas for the first time. The results showed the quality of ginseng roots and rhizomes from different sources was different due to growing environment, cultivation technology, and so on. The developed LC-ESI-IT-TOF-MS n method can be used to identify many more ginsenosides and the LC-DAD method can be used not only to assess the quality of ginseng, but also to optimize the cultivation conditions for the production of ginsenosides.

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Section: Introductionmentioning

confidence: 99%

High-Performance Liquid Chromatography with Diode Array Detector and Electrospray Ionization Ion Trap Time-of-Flight Tandem Mass Spectrometry to Evaluate Ginseng Roots and Rhizomes from Different Regions

et al. 2016

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“…In the present study, the papain hydrolysis of bovine blood-derived fibrinogen or cglobulin resulted in the generation of hydrolysates that significantly decreased cell proliferation in various different cancer cell lines (Tables 1 and 2). Peptides, isolated from the herb Panax ginseng, have also been reported to have lower IC 50 values in suspension cells (human peripheral blood cells) compared to adherent (human gastric carcinoma cells) (Ma & Yang, 2015). U937 cells grow in suspension unlike the other cell lines used in this experiment.…”

Section: Discussionmentioning

confidence: 87%

“…Adherent oral cancer cells were grown either under normal cell culture conditions or on a 3D matrix, and it was reported that anti-cancer drugs were more toxic to cells in the 3D matrix (Adcock et al, 2015). Peptides, isolated from the herb Panax ginseng, have also been reported to have lower IC 50 values in suspension cells (human peripheral blood cells) compared to adherent (human gastric carcinoma cells) (Ma & Yang, 2015). Interestingly, results from Adcock et al (2015) found that prostate cancer cells were less sensitive in a 3D model compared to adherent cell culture conditions which may indicate cell-to-cell variation (Adcock et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Anti‐proliferative activity of bovine blood hydrolysates towards cancer cells in culture

O'Sullivan

Lafarga

Hayes

et al. 2017

Int J of Food Sci Tech

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Four proteins, α/β globulin, serum albumin, γ‐globulin and fibrinogen, were isolated from bovine blood and hydrolysed using papain. Hydrolysates were assessed for non‐cellular and cellular antioxidant activity. The anti‐proliferative activity of hydrolysed fractions was assessed in a number of cancer cell lines including U937 lymphoma cells, MCF‐7 breast cancer cells, HepG2 hepatocytes and Caco‐2 epithelial colorectal adenocarcinoma cells. Anti‐inflammatory activity of the hydrolysates was also assessed. Hydrolysates generated from γ‐globulin or fibrinogen had significant antioxidant activity in non‐cellular assays. Hydrolysates were also found to be highly toxic to different cancer cell lines, in particular U937 lymphoma cells when assessed using the MTT assay. The fibrinogen hydrolysate was the most toxic sample and toxicity appeared to correlate with its non‐cellular antioxidant activity. None of the hydrolysates had significant anti‐inflammatory activity. The high cytotoxicity of the γ‐globulin and the fibrinogen hydrolysates towards cancer cells may indicate a potential use as anti‐proliferative agents.

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“…The results suggested that DP37 and DP38 were possibly formed by the addition of one molecule of H 2 O to PPD and 20(R)‐PPD. DP37 and DP38 were tentatively identified as 25‐OH‐20(S)‐PPD and 25‐OH‐20(R)‐PPD, respectively .…”

Section: Resultsmentioning

confidence: 99%

Identification and characterization of forced degradation products and stability‐indicating assay for notoginsenosidefc by using UHPLC‐Q‐TOF‐MS and UHPLC‐MS/MS: Insights into stability profile and degradation pathways

Ma¹,

Guan²,

Ju³

et al. 2019

J of Separation Science

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Notoginsenoside Fc, a protopanaxadiol-type saponin, shows multi-pharmacological activities. Chemical stability evaluation plays a crucial role in drug development.In this study, the forced degradation behavior of Notoginsenoside Fc was investigated under hydrolytic and oxidative conditions. A specific ultra high performance liquid chromatography with quadrupole time-of-flight mass spectrometry was developed for the separation, identification, and characterization of the degradation products of Notoginsenoside Fc. Fifty potential degradation products were formed via deglycosylation, dehydration, hydration, isomerization, side-chain cleaving, oxidation, and superoxidation. Notoginsenoside Fc was subjected to different pH solutions, temperatures, and time periods to assess its stability. A sensitive ultra high performance liquid chromatography-tandem mass spectrometry was developed for the quantification of Notoginsenoside Fc, notoginsenoside ST-4, notoginsenoside Ft1, and relative quantification of notoginsenoside Ft2, 20(R)-notoginsenoside Ft2, notoginsenoside SFt3, and notoginsenoside SFt4. The assay was linear over the concentration range (R 2 > 0.997) with the lowest limit of quantification of 0.02 μg/mL for Notoginsenoside Fc, Notoginsenoside ST-4, and Notoginsenoside Ft1. The intra-day precision, inter-day precision, and accuracy of the three analytes were within accepted levels. The degradation kinetics of Notoginsenoside Fc in pH 1 and 3 solutions fits to first-and second-order kinetics, respectively. The degradation of Notoginsenoside Fc is pH-, temperature-, and time-dependent.

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Six new dammarane-type triterpenes from acidic hydrolysate of the stems-leaves of Panax ginseng and their inhibitory–activities against three human cancer cell lines

Cited by 20 publications

References 27 publications

High-Performance Liquid Chromatography with Diode Array Detector and Electrospray Ionization Ion Trap Time-of-Flight Tandem Mass Spectrometry to Evaluate Ginseng Roots and Rhizomes from Different Regions

High-Performance Liquid Chromatography with Diode Array Detector and Electrospray Ionization Ion Trap Time-of-Flight Tandem Mass Spectrometry to Evaluate Ginseng Roots and Rhizomes from Different Regions

Anti‐proliferative activity of bovine blood hydrolysates towards cancer cells in culture

Identification and characterization of forced degradation products and stability‐indicating assay for notoginsenosidefc by using UHPLC‐Q‐TOF‐MS and UHPLC‐MS/MS: Insights into stability profile and degradation pathways

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