Six‐gene Assay as a new biomarker in the blood of patients with colorectal cancer: establishment and clinical validation

Shou, Xin; Li, Yong; Hu, Wenquan; Ye, Tingting; Wang, Guosheng; Xu, Feng; Sui, Meihua; Xu, Yan

doi:10.1002/1878-0261.12427

Cited by 13 publications

(11 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because Kelch-like ECH-associated protein 1 (Keap1)/Nrf2 signaling is a key mediator in thyroid pathophysiology and thyroid cancer, the regulation of AOX1 by the Keap/Nrf2 pathway might account for part of the pathogenesis of PTC (38). Both gene expression in tissues and protein contents in plasma have promising diagnostic and prognostic potential in many tumors (39)(40)(41). A previously study reported that serum TPO was not a useful marker for patients with differentiated thyroid carcinoma (42).…”

Section: Discussionmentioning

confidence: 99%

Clinical implications of TPO and AOX1 in pediatric papillary thyroid carcinoma

Wang

Zhang

et al. 2021

Transl Pediatr

View full text Add to dashboard Cite

Background: Thyroid carcinoma is a common pediatric head and neck cancer, of which papillary thyroid cancer (PTC) is the most common type. Previously, we found that thyroid peroxidase (TPO) and aldehyde oxidase 1 (AOX1) were differentially expressed in PTC. This study explored the clinical importance of TPO and AOX1 in the diagnosis and prognosis of PTC in children.Methods: Both TPO and AOX1 expression in PTC were analyzed using datasets from Gene Expression Omnibus (GEO). TPO and AOX1 protein levels in plasma from patients with PTC and non-tumor controls were detected via enzyme-linked immunosorbent assay (ELISA). The diagnostic accuracy of TPO and AOX1 was assessed using receiver operating characteristic (ROC) curve analysis. The association between gene expression levels and patient survival was explored using the Kaplan-Meier plotter online database. Results:The results revealed that TPO and AOX1 expression was significantly downregulated in four independent datasets (GSE33630, GSE27155, GSE3678, and GSE3467). TPO and AOX1 protein levels in blood plasma were significantly decreased in patients with PTC. Quantitative analysis demonstrated that TPO and AOX1 levels in plasma had satisfactory predictive performance and the ability to discriminate PTC from healthy samples. Prognostic analysis demonstrated that low levels of TPO and AOX1 were markedly associated with poor survival in patients with PTC.Conclusions: In summary, these results implied that TPO and AOX1 could serve as novel biomarkers for the diagnosis and prognosis of pediatric PTC.

show abstract

Section: Discussionmentioning

confidence: 99%

Clinical implications of TPO and AOX1 in pediatric papillary thyroid carcinoma

Wang

Zhang

et al. 2021

Transl Pediatr

View full text Add to dashboard Cite

show abstract

“…Gene expression has promising diagnostic potential in a number of types of cancer, including colorectal, ovarian, and breast cancer (43–45). In the present study, the calculated AUC values ranged from 0.7–0.9, which is considered an excellent discriminatory power (46), while the AUC values for CDH6, COL13A1, ADH1B and TBC1D4 were increased.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis to screen key genes in papillary thyroid carcinoma

et al. 2019

View full text Add to dashboard Cite

Papillary thyroid carcinoma (PTC) is the most common type of thyroid carcinoma, and its incidence has been on the increase in recent years. However, the molecular mechanism of PTC is unclear and misdiagnosis remains a major issue. Therefore, the present study aimed to investigate this mechanism, and to identify key prognostic biomarkers. Integrated analysis was used to explore differentially expressed genes (DEGs) between PTC and healthy thyroid tissue. To investigate the functions and pathways associated with DEGs, Gene Ontology, pathway and protein-protein interaction (PPI) network analyses were performed. The predictive accuracy of DEGs was evaluated using the receiver operating characteristic (ROC) curve. Based on the four microarray datasets obtained from the Gene Expression Omnibus database, namely GSE33630, GSE27155, GSE3467 and GSE3678, a total of 153 DEGs were identified, including 66 upregulated and 87 downregulated DEGs in PTC compared with controls. These DEGs were significantly enriched in cancer-related pathways and the phosphoinositide 3-kinase-AKT signaling pathway. PPI network analysis screened out key genes, including acetyl-CoA carboxylase beta, cyclin D1, BCL2, and serpin peptidase inhibitor clade A member 1, which may serve important roles in PTC pathogenesis. ROC analysis revealed that these DEGs had excellent predictive performance, thus verifying their potential for clinical diagnosis. Taken together, the findings of the present study suggest that these genes and related pathways are involved in key events of PTC progression and facilitate the identification of prognostic biomarkers.

show abstract

“…Colorectal cancer (CRC), as the most popular cancer around the world, is seriously endangering the health of all humankind. Its incidence rate ranks second in men and third in women [ 1 ]. According to cancer statistics in the United States, about 147,950 people were diagnosed with CRC and nearly 53,200 people died of CRC in 2020 [ 2 ].…”

Section: Highlightsmentioning

confidence: 99%

Long non-coding RNA KCNQ1OT1 up-regulates CTNND1 by sponging miR-329-3p to induce the proliferation, migration, invasion, and inhibit apoptosis of colorectal cancer cells

et al. 2020

View full text Add to dashboard Cite

Background Long non-coding RNAs (lncRNAs) have been certified to be involved in the occurrence and growth of diverse cancers, including CRC. The purpose of the research was to explore the effects of lncRNA KCNQ1 overlapping transcript 1 (KCNQ1OT1) on proliferation, migration, invasion, and apoptosis in CRC cells and its mechanism. Methods The levels of KCNQ1OT1 and miR-329-3p were examined by quantitative real-time polymerase chain reaction (qRT-PCR) in CRC tissues and cells. The mRNA and protein levels of catenin delta-1 (CTNND1) were measured by qRT-PCR and western blot analysis, respectively. The targets of KCNQ1OT1 and miR-329-3p were predicted by online software and confirmed by luciferase reporter assay. The cell proliferation, migration, invasion, and apoptosis were examined using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), transwell, and apoptosis assay. The expression levels of CyclinD1, Bcl-2, MMP9, Cleaved-casp-3, and E-cadherin in SW480 and LS1034 cells were gauged by western blot analysis. Xenograft tumor model was structured to prove the biological role of KCNQ1OT1 of CRC in vivo. Results The levels of KCNQ1OT1 and CTNND1 were significantly increased in CRC tissues and cells. Knockdown of KCNQ1OT1 suppressed proliferation, migration, invasion, and induced apoptosis in CRC cells. Conversely, CTNND1 overexpression reversed the impact of KCNQ1OT1 knockdown on CRC cells. Moreover, CTNND1 was verified as a direct target of miR-329-3p, and miR-329-3p could specially bind to KCNQ1OT1. Also, the down-regulation of KCNQ1OT1 triggered the CRC progress by up-regulating CTNND1 expression in CRC cells. Besides, KCNQ1OT1 knockdown inhibited CRC tumor growth through the miR-329-3p/CTNND1 axis in vivo. Conclusion Our results indicated that KCNQ1OT1 could positively regulate CTNND1 expression by sponging miR-329-3p, thereby boosting the progression of CRC. Our findings provided the underlying therapy targets for CRC.

show abstract

Six‐gene Assay as a new biomarker in the blood of patients with colorectal cancer: establishment and clinical validation

Cited by 13 publications

References 34 publications

Clinical implications of TPO and AOX1 in pediatric papillary thyroid carcinoma

Clinical implications of TPO and AOX1 in pediatric papillary thyroid carcinoma

Bioinformatics analysis to screen key genes in papillary thyroid carcinoma

Long non-coding RNA KCNQ1OT1 up-regulates CTNND1 by sponging miR-329-3p to induce the proliferation, migration, invasion, and inhibit apoptosis of colorectal cancer cells

Contact Info

Product

Resources

About