Site-specific phosphorylation of TRANSPARENT TESTA GLABRA1 mediates carbon partitioning in Arabidopsis seeds

Li, Chengxiang; Zhang, Bin; Chen, Bin; Ji, Lianghui; Yu, Hao

doi:10.1038/s41467-018-03013-5

Cited by 56 publications

(70 citation statements)

References 50 publications

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“…TRANSPARENT TESTA 8 (TT8), for instance, can directly inhibit the transcription of LEC1, LEC2, and FUS3 (Chen et al, 2014). This basic helix-loop-helix (bHLH) TF can form a ternary complex with an R2R3-MYB factor TT2 and a WD40 repeat factor TRANSPARENT TESTA GLABRA 1 (TTG1) to monitor carbon partitioning among metabolic pathways that share the same source, including seed oil, mucilage and flavonoid synthesis (Li et al, 2018). Interestingly, there is a TT2-FUS3-TTG1 cascade linked via direct gene suppression (Wang et al, 2014;Chen et al, 2015).…”

Section: Transcriptional Factorsmentioning

confidence: 99%

“…The repressor TTG1 is regulated by shaggy-like kinases 11/12 (SK11/12) that belongs to the glycogen synthase kinase-3 (GSK3) family (Li et al, 2018). Of note, SK11 (or ASKα) can be induced by salt stress (Dal Santo et al, 2012).…”

Section: Signaling Pathwaysmentioning

confidence: 99%

“…TTG1, together with TT2 and TT8, coordinates carbon partitioning. However, they drive carbon flux into the synthetic pathways of seed coat mucilage and flavonoid pigments, which compete with oil for photosynthates (Li et al, 2018). One of their targets is GLABRA2 (GL2).…”

Section: Signaling Pathwaysmentioning

confidence: 99%

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Plant Unsaturated Fatty Acids: Biosynthesis and Regulation

et al. 2020

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In most plants, major unsaturated fatty acids (UFAs) are three C18 species, namely, oleic (18:1), linoleic (18:2), and α-linolenic (18:3) acids. These simple compounds play multiple crucial roles in planta and are also important economic traits of oil crops. The enzymatic steps of C18 UFA biosynthesis have been well established. However, the associated FA/lipid trafficking between the plastid and the endoplasmic reticulum remains largely unclear, as does the regulation of the expression and activities of the involved enzymes. In this review, we will revisit the biosynthesis of C18 UFAs with an emphasis on the trafficking, and present an overview of the key enzymes and their regulation. Of particular interest is the emerging regulatory network composed of transcriptional factors and upstream signaling pathways. The review thereby provides the promise of using physical, biochemical and/or genetic means to manipulate FA composition and increase oil yield in crop improvement.

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Section: Transcriptional Factorsmentioning

confidence: 99%

Section: Signaling Pathwaysmentioning

confidence: 99%

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Plant Unsaturated Fatty Acids: Biosynthesis and Regulation

et al. 2020

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“…The bes1 bzr1 loss-of-function mutants and bes1-D bzr1-D gain-of-function mutants were as reported previously 34 . pSK11:GUS and pSK12:GUS lines were as reported previously 25 .…”

Section: Methodsmentioning

confidence: 99%

VISUAL-CC system uncovers the role of GSK3 as an orchestrator of vascular cell type ratio in plants

et al. 2020

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The phloem transports photosynthetic assimilates and signalling molecules. It mainly consists of sieve elements (SEs), which act as "highways" for transport, and companion cells (CCs), which serve as "gates" to load/unload cargos. Though SEs and CCs function together, it remains unknown what determines the ratio of SE/CC in the phloem. Here we develop a new culture system for CC differentiation in Arabidopsis named VISUAL-CC, which almost mimics the process of the SE-CC complex formation. Comparative expression analysis in VISUAL-CC reveals that SE and CC differentiation tends to show negative correlation, while total phloem differentiation is unchanged. This varying SE/CC ratio is largely dependent on GSK3 kinase activity. Indeed, gsk3 hextuple mutants possess many more SEs and fewer CCs, whereas gsk3 gain-of-function mutants partially increase the CC number. Taken together, GSK3 activity appears to function as a cell-fate switch in the phloem, thereby balancing the SE/CC ratio.

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“…Previous studies have revealed that the vascular cells express SKII subgroup genes BIN2 and BIN2-LIKE2 (BIL2) 24 . In addition, expression of SKI subgroup genes pSK11:GUS and pSK12:GUS 25 was found in the vasculature including the phloem tissue (Fig. 4f).…”

Section: Genetic Manipulation Of Gsk3 Activity Alters the In Vivo Se/mentioning

confidence: 97%

GSK3 activity is a cell fate switch that balances the ratio of vascular cell type

Tamaki

Oya

Naito

et al. 2020

Preprint

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The phloem transports photosynthetic assimilates and signalling molecules. It mainly consists of sieve elements (SEs), which act as "highways" for transport, and companion cells (CCs), which serve as "gates" to load/unload cargos. Though SEs and CCs function together, it remains unknown what determines the ratio of SE /CC in the phloem. In this study, we develop a novel culture system for CC differentiation named VISUAL-CC, which reconstitutes the SE-CC complex formation. Comparative expression analysis in VISUAL-CC reveals that SE and CC differentiation tends to show negative correlation, while total phloem differentiation is unchanged. This varying SE/CC ratio is largely dependent on GSK3 kinase activity. Indeed, gsk3 hextuple mutants possess much more SEs and less CCs in planta. Conversely, gsk3 gain-of-function mutants induced by phloem-specific promoter partially increased the CC ratio. Taken together, GSK3 activity appears to function as a cell fate switch in the phloem, thereby balancing the SE/CC ratio.

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Site-specific phosphorylation of TRANSPARENT TESTA GLABRA1 mediates carbon partitioning in Arabidopsis seeds

Cited by 56 publications

References 50 publications

Plant Unsaturated Fatty Acids: Biosynthesis and Regulation

Plant Unsaturated Fatty Acids: Biosynthesis and Regulation

VISUAL-CC system uncovers the role of GSK3 as an orchestrator of vascular cell type ratio in plants

GSK3 activity is a cell fate switch that balances the ratio of vascular cell type

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