Site-specific effects of intracerebral injections of three neurokinins (neurokinin A, neurokinin K, and neurokinin γ) on the expression of male rat sexual behavior

Dornan, Wayne A.; Vink, Krista L.; Malen, Peter; Short, Kevin R.; Struthers, William M.; Barrett, Christopher

doi:10.1016/0031-9384(93)90107-q

Cited by 15 publications

(8 citation statements)

References 54 publications

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“…Final values calculated as pmol/l to facilitate comparisons with other assays (Stronge et al 2008). iii) Neurokinin ELISA (#EIA-NEA1, Raybiotech, Norcross, GA, USA) has an assay range of 0.8-1000 pg/ml with a published sensitivity of 0.8 pg/ml (Dornan et al 1993). The intra-and interassay CV values are !10 and !15%, respectively.…”

Section: Multianalyte Algorithm Analysis (Maaa) Pcr-based Test (Netest)mentioning

confidence: 99%

A multianalyte PCR blood test outperforms single analyte ELISAs (chromogranin A, pancreastatin, neurokinin A) for neuroendocrine tumor detection

Modlin

Drozdov

Alaimo

et al. 2014

Endocrine-Related Cancer

103

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A critical requirement in neuroendocrine tumor (NET) management is a sensitive, specific and reproducible blood biomarker test. We evaluated a PCR-based 51 transcript signature (NETest) and compared it to chromogranin A (CgA), pancreastatin (PST) and neurokinin A (NKA). The multigene signature was evaluated in two groups: i) a validation set of 40 NETs and controls and ii) a prospectively collected group of NETs (nZ41, 61% small intestinal, 50% metastatic, 44% currently treated and 41 age-sex matched controls). Samples were analyzed by a two-step PCR (51 marker genes) protocol and ELISAs for CgA, PST and NKA. Sensitivity comparisons included c 2 , non-parametric measurements, ROC curves and predictive feature importance (PFAI) analyses. NETest identified 38 of 41 NETs. Performance metrics were: sensitivity 92.8%, specificity 92.8%, positive predictive value 92.8% and negative predictive value 92.8%. Single analyte ELISA metrics were: CgA 76, 59, 65, and 71%; PST 63, 56, 59, and 61% and NKA 39, 93, 84, and 60%. The AUCs (ROC analysis) were: NETest: 0.96G0.025, CgA: 0.67G0.06, PST 0.56G0.06, NKA: 0.66G0.06. NETest significantly outperformed single analyte tests (area differences: 0.284-0.403, Z-statistic 4.85-5.9, P!0.0001). PFAI analysis determined NETest had most value (69%) in diagnosis (CgA (13%), PST (9%), and NKA (9%)). Test data were consistent with the validation set (NETest O95% sensitivity and specificity, AUC Z0.98 vs single analytes: 59-67% sensitivity, AUCs: 0.58-0.63). The NETest is significantly more sensitive and efficient (O93%) than single analyte assays (CgA, PST or NKA) in NET diagnosis. Blood-based multigene analytic measurement will facilitate early detection of disease recurrence and can predict therapeutic efficacy.

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Section: Multianalyte Algorithm Analysis (Maaa) Pcr-based Test (Netest)mentioning

confidence: 99%

A multianalyte PCR blood test outperforms single analyte ELISAs (chromogranin A, pancreastatin, neurokinin A) for neuroendocrine tumor detection

Modlin

Drozdov

Alaimo

et al. 2014

Endocrine-Related Cancer

103

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“…In our initial experiments we examined whether applying NPK to the ventromedial nucleus of the hypothalamus could inhibit maternal behavior. We chose to examine NPK because previous work had shown that the central administration of NPK had potent inhibitory effects on male sexual behavior in rats 66 …”

Section: The Aversion/avoidance Systemmentioning

confidence: 99%

Neuroanatomical Circuitry for Mammalian Maternal Behavior^a

Numan

Sheehan

1997

Annals of the New York Academy of Sciences

185

147

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“…Neurokinins are known to play a number of roles in mammalian reproduction, including modulation of smooth muscle contractility of the uterus of rats (Moodley et al, 1999), and humans (Patak et al, 2003), the influencing of sexual behavioral responses in rats (Dornan et al, 1993), and the hormone-dependent support of reproductive organ development and maintenance in mice and rats (Pintado et al, 2003). The latter effect has been attributed to the role of NKs as paracrine effectors involved in modulating reproductive hormonal interactions.…”

Section: Discussionmentioning

confidence: 99%

Administration of an Antagonist of Neurokinin Receptors 1, 2, and 3 Results in Reproductive Tract Changes in Beagle Dogs, but Not Rats

Losco¹,

Leach²,

Sinha³

et al. 2007

Toxicol Pathol

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SCH 206272, an antagonist of neurokinin receptors 1, 2, and 3, was administered orally by gavage for 1 month to 8-to 10-month-old dogs at doses of 0, 15, 30, or 60 mg/kg, and to 6-week-old rats at doses of 0, 30, 100, or 300 mg/kg. The most important changes occurred in the reproductive tract of the dogs at all doses. Absolute and relative group mean organ weights for the testes, prostate gland, epididymides, ovaries, and uterus were 33-86% lower than concurrent controls in groups receiving SCH 206272. Organ weight changes were not dose-related. Microscopic changes that correlated with the organ weight changes occurred in all groups receiving SCH 206272. For males, they included minimal to severe atrophy of the testes, epididymides, and prostate gland. In addition, the epididymides exhibited severe oligospermia or aspermia, minimal epithelial apoptosis and mild epithelial vacuolation. In female dogs, the ovaries and uteri appeared immature. Microscopic changes were similar in incidence and severity in dogs receiving 30 or 60 mg/kg, but were slightly less in dogs receiving 15 mg/kg. In contrast, similar findings were not observed in the reproductive tract of male or female rats, despite overlapping systemic, hypothalamic, and pituitary gland concentrations of SCH 206272.

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Site-specific effects of intracerebral injections of three neurokinins (neurokinin A, neurokinin K, and neurokinin γ) on the expression of male rat sexual behavior

Cited by 15 publications

References 54 publications

A multianalyte PCR blood test outperforms single analyte ELISAs (chromogranin A, pancreastatin, neurokinin A) for neuroendocrine tumor detection

A multianalyte PCR blood test outperforms single analyte ELISAs (chromogranin A, pancreastatin, neurokinin A) for neuroendocrine tumor detection

Neuroanatomical Circuitry for Mammalian Maternal Behavior^a

Administration of an Antagonist of Neurokinin Receptors 1, 2, and 3 Results in Reproductive Tract Changes in Beagle Dogs, but Not Rats

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Site-specific effects of intracerebral injections of three neurokinins (neurokinin A, neurokinin K, and neurokinin γ) on the expression of male rat sexual behavior

Cited by 15 publications

References 54 publications

A multianalyte PCR blood test outperforms single analyte ELISAs (chromogranin A, pancreastatin, neurokinin A) for neuroendocrine tumor detection

A multianalyte PCR blood test outperforms single analyte ELISAs (chromogranin A, pancreastatin, neurokinin A) for neuroendocrine tumor detection

Neuroanatomical Circuitry for Mammalian Maternal Behaviora

Administration of an Antagonist of Neurokinin Receptors 1, 2, and 3 Results in Reproductive Tract Changes in Beagle Dogs, but Not Rats

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Resources

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Neuroanatomical Circuitry for Mammalian Maternal Behavior^a