Site-dependent induction of jasmonic acid-associated chemical defenses against western flower thrips in Chrysanthemum

Chen, Gang; Kim, Hye Kyong; Klinkhamer, Peter G. L.; Escobar‐Bravo, Rocío

doi:10.1007/s00425-019-03292-2

Cited by 18 publications

(16 citation statements)

References 66 publications

(75 reference statements)

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“…Additional correlational analysis between chlorogenic acid and PPO activity levels on a larger number of genotypes should be performed in future studies. Activation of JA signaling has been reported to enhance plant resistance to WFT in some plant species (Abe et al 2009;Chen et al 2018;Escobar-Bravo et al 2017;Li et al 2002), including chrysanthemum (Chen et al 2020). Our study further reveals that the effect of JA on chrysanthemum resistance to WFT is genotype-dependent (Fig.…”

Section: Discussionsupporting

confidence: 75%

“…Taken together, the chemical or physical traits that contribute to JA-mediated induction of chrysanthemum resistance to WFT are still unknown. However, in a recent study, variations in JA-mediated induction of sugars and amino acids, as well as of defensive chemicals such as chlorogenic acid, coincided with differences in thrips susceptibility between young and old leaves of chrysanthemum plants (Chen et al 2020 ). These compounds could be targets for future studies.…”

Section: Discussionmentioning

confidence: 99%

“…Activation of JA signaling has been reported to enhance plant resistance to WFT in some plant species (Abe et al 2009 ; Chen et al 2018 ; Escobar-Bravo et al 2017 ; Li et al 2002 ), including chrysanthemum (Chen et al 2020 ). Our study further reveals that the effect of JA on chrysanthemum resistance to WFT is genotype-dependent (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…PPO activity was determined in the third leaf from the bottom following the methodology described in Stout et al ( 1998 ). This leaf was chosen based on previous work that showed a stronger inducibility of PPO levels in basal chrysanthemum leaves (Chen et al 2020 ). Briefly, 0.150 g of leaf tissue without midrib was flash frozen in liquid nitrogen, ground with a tissue-lyser (Qiagen, Hilden, Germany) and homogenized in a 2 ml tube with 1.25 ml ice-cold 0.1 M pH 7.0 phosphate buffer containing 7% polyvinylpolypyrolidine and 0.4 ml of 10% Triton X-100.…”

Section: Methodsmentioning

confidence: 99%

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Constitutive and Inducible Resistance to Thrips Do Not Correlate With Differences in Trichome Density or Enzymatic-Related Defenses in Chrysanthemum

2020

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Western flower thrips (WFT), Frankliniella occidentalis, is a serious insect pest of Chrysanthemum [Chrysanthemum × morifolium Ramat. (Asteraceae)]. Here we have investigated whether genotypic variation in constitutive and inducible resistance to WFT correlates with phenotypic differences in leaf trichome density and the activity of the defense-related enzyme polyphenol oxidase (PPO) in chrysanthemum. Non-glandular and glandular leaf trichome densities significantly varied among ninety-five chrysanthemum cultivars. Additional analyses in a subset of these cultivars, differing in leaf trichome density, revealed significant variation in PPO activities and resistance to WFT as well. Constitutive levels of trichome densities and PPO activity, however, did not correlate with chrysanthemum resistance to WFT. Further tests showed that exogenous application of the phytohormone jasmonic acid (JA) increased non-glandular trichome densities, PPO activity and chrysanthemum resistance to WFT, and that these effects were cultivar dependent. In addition, no tradeoff between constitutive and inducible resistance to WFT was observed. JA-mediated induction of WFT resistance, however, did not correlate with changes in leaf trichome densities nor PPO activity levels. Taken together, our results suggest that chrysanthemum can display both high levels of constitutive and inducible resistance to WFT, and that leaf trichome density and PPO activity may not play a relevant role in chrysanthemum defenses against WFT.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Constitutive and Inducible Resistance to Thrips Do Not Correlate With Differences in Trichome Density or Enzymatic-Related Defenses in Chrysanthemum

2020

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“…One constraint in breeding plants to defend against plant herbivores and pathogens has generally been the need to combine several analytical methods to obtain an overview of total secondary metabolism (Wink 1988). Ecometabolomic analyses are excellent tools to advance the study of effective treatments against herbivorous pests in plants (Chen et al 2020). Chen et al (2020) recently reported that the application of jasmonic acid to all leaves or only the apical leaves of chrysanthemums (Chrysanthemum × morifolium) increased the resistance to thrips, whereas the application to basal leaves was not v www.esajournals.org effective against the thrip pest (Chen et al 2020).…”

Section: General and Significant Changes Observed In Metabolomic Analyses Of Plant Foliar Metabolism Related Tomentioning

confidence: 99%

Ecometabolomics of plant–herbivore and plant–fungi interactions: a synthesis study

et al. 2021

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The functional adaptive responses of higher plants to biotic interactions with herbivores and fungi have long been topics of research. One constraint to obtaining a comprehensive understanding of the most general plant responses, however, has been the difficulty of studying all plant functional shifts simultaneously due to analytical limitations. Now this is possible with the advent of metabolomics. Using 151 records from the WEB of SCIENCE database, we have analyzed the development and application of metabolomic profiles to ecological studies in the last two decades. We have used meta-analysis and pathway enrichment analyses to assess the whole set of constitutive and inducible defenses. Constitutive defenses against herbivores were mainly based on a significant high level of the metabolism of several amino acids with parallel increases in the concentrations of flavones (phenolics) and saponins (glycosides). Inducible defenses, though, were mainly based on the increases in concentration of methyl-ketone, pantothenate, and Coenzyme A. Butyrate metabolism and the mitochondrial electron-transport chain were upregulated, in agreement with previous reports that herbivory-activated plant chemical defenses were mainly based on jasmonic acid, salicylic acid, and ethylene-associated pathways. The metabolic responses/acclimations to pathogenic fungi were mainly linked with increases in aspartate and pyruvate metabolism, the transfer of acetyl groups within mitochondria, and the upregulation of branched-chain amino acids degradation pathways. These responses/acclimations were accompanied by higher concentrations of the most important groups of secondary metabolites such as phenolics (anthocyanins, flavonoids), quinones, alkaloids, terpenoids, and polyamines and other compounds related to antistress mechanisms such as proline. The leaves of mycorrhized plants accumulated nucleotide sugars, sphingolipids, and methylhistidine. These responses were associated with maintaining the integrity of plant cell membranes under fungal hypha penetration. The responses were accompanied by increases in the concentrations of phenolics, blumenols, and alkaloids and decreases in the concentrations of polyamines, consistent with the mycorrhizal inhibition of polyamines. This summary provides a clear synthesis of the most successful plant strategies selected after millions of years of evolution and will be a very promising tool for the management of crops and ecosystems and for selecting the main lines in breeding studies for future research.

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Effect of stress hormones on the metabolome of a suspension culture of the aromatic medicinal plant Cymbopogon schoenanthus subsp. proximus

Abdelsalam

Chowdhury

Boroujerdi

et al. 2023

Plant Cell Tiss Organ Cult

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The current study aimed to evaluate and compare the effect of different concentrations (0.0–200.0 µM) of jasmonic acid (JA), methyl jasmonate (MeJA), and salicylic acid (SA) on suspension culture mass production and untargeted metabolic profiling of the medicinal plant Cymbopogon schoenanthus subsp. proximus. The addition of 50 µM MeJA improved the fresh weight of embryogenic tissue significantly. MeJA and SA did not affect tissue dry weight, whereas JA significantly decreased it. Based on 1H and 1H–13C NMR data and NMR databases, 50 compounds were identified. The addition of stress hormones resulted in the biosynthesis of novel metabolites like theophylline and syringate that were absent in control samples. In addition, significant variations in the concentrations of numerous compounds, including sugars, amino acids, organic acids, phenols, and alkaloids, were observed. The upregulation of trigonelline concentration was observed upon the addition of a higher concentration of MeJA (200 µM), whereas all tested concentrations of SA resulted in its upregulation. Addition of JA and SA causes significant changes in aminoacyl-tRNA biosynthesis pathway and amino acid metabolism pathways, such as alanine-aspartate and glutamate metabolism and arginine and proline metabolism. MeJA had significant impacts on glycolysis and starch-glucose metabolism pathways in addition to amino acids metabolism pathways. The present findings were successful in demonstrating a correlation and distinction between the effects of JA, MeJA, and SA, on the metabolome of Cymbopogon schoenanthus, a valuable medicinal plant. The identified metabolites and their associated pathways would be valuable in future biotechnology applications of the genus Cymbopogon.

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Site-dependent induction of jasmonic acid-associated chemical defenses against western flower thrips in Chrysanthemum

Abstract: Main conclusion Local and systemic induction of JA-associated chemical defenses and resistance to western flower thrips in Chrysanthemum are spatially variable and dependent on the site of the JA application.

Cited by 18 publications

References 66 publications

Constitutive and Inducible Resistance to Thrips Do Not Correlate With Differences in Trichome Density or Enzymatic-Related Defenses in Chrysanthemum

Constitutive and Inducible Resistance to Thrips Do Not Correlate With Differences in Trichome Density or Enzymatic-Related Defenses in Chrysanthemum

Ecometabolomics of plant–herbivore and plant–fungi interactions: a synthesis study

Effect of stress hormones on the metabolome of a suspension culture of the aromatic medicinal plant Cymbopogon schoenanthus subsp. proximus

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